Metabolomic analysis indicated changes in triacylglycerols’ levels as a result of training in Whippet dogs
Abstract Regular physical effort produces metabolic changes manifested as adaptation to exercise and increasing performance. In humans these changes have been characterized at metabolome level as depending on the discipline. However, all sports involve some level of changes in protein, carbohydrate...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45546-w |
| _version_ | 1797452798870159360 |
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| author | Katarzyna Miazga Klaudia Kopczyńska Olga Szaluś-Jordanow Agata Moroz-Fik Jacek Wilczak Karolina Barszcz Anna Cywińska |
| author_facet | Katarzyna Miazga Klaudia Kopczyńska Olga Szaluś-Jordanow Agata Moroz-Fik Jacek Wilczak Karolina Barszcz Anna Cywińska |
| author_sort | Katarzyna Miazga |
| collection | DOAJ |
| description | Abstract Regular physical effort produces metabolic changes manifested as adaptation to exercise and increasing performance. In humans these changes have been characterized at metabolome level as depending on the discipline. However, all sports involve some level of changes in protein, carbohydrate and lipid metabolism. Recently, also performance horses have been subjected to metabolic analyses, but similar studies were lacking in sports dogs. In this study we performed the metabolomic analysis in plasma of Whippet dogs regularly trained and competing in coursing events, and untrained dogs of the same breed, fed with the same diet. We have also compared the hematological and blood biochemical results in these two groups of dogs. Basic blood tests indicated that enzymes related to lipid metabolism (lipase and gamma-glutamyltransferase) differed considerably between the groups. Metabolomic analysis of plasma confirmed the metabolic shift expressed as the differences in triacylglycerols levels between training and non-training dogs, aimed at improving the use of fatty acids as a source of energy during exertion. Surprisingly, other classes of metabolites were only hardly changed when comparing training and non-training Whippets. |
| first_indexed | 2024-03-09T15:13:53Z |
| format | Article |
| id | doaj.art-a1fc37576d3d4550ba6e515ac3b96e87 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:13:53Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a1fc37576d3d4550ba6e515ac3b96e872023-11-26T13:13:57ZengNature PortfolioScientific Reports2045-23222023-10-0113111010.1038/s41598-023-45546-wMetabolomic analysis indicated changes in triacylglycerols’ levels as a result of training in Whippet dogsKatarzyna Miazga0Klaudia Kopczyńska1Olga Szaluś-Jordanow2Agata Moroz-Fik3Jacek Wilczak4Karolina Barszcz5Anna Cywińska6Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGWDepartment of Functional and Organic Food, Institute of Human Nutrition Sciences, Warsaw University of Life SciencesDepartment of Small Animal Diseases with Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGWDivision of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGWDepartment of Physiology, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGWDepartment of Morphological Sciences, Warsaw University of Life SciencesFaculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in TorunAbstract Regular physical effort produces metabolic changes manifested as adaptation to exercise and increasing performance. In humans these changes have been characterized at metabolome level as depending on the discipline. However, all sports involve some level of changes in protein, carbohydrate and lipid metabolism. Recently, also performance horses have been subjected to metabolic analyses, but similar studies were lacking in sports dogs. In this study we performed the metabolomic analysis in plasma of Whippet dogs regularly trained and competing in coursing events, and untrained dogs of the same breed, fed with the same diet. We have also compared the hematological and blood biochemical results in these two groups of dogs. Basic blood tests indicated that enzymes related to lipid metabolism (lipase and gamma-glutamyltransferase) differed considerably between the groups. Metabolomic analysis of plasma confirmed the metabolic shift expressed as the differences in triacylglycerols levels between training and non-training dogs, aimed at improving the use of fatty acids as a source of energy during exertion. Surprisingly, other classes of metabolites were only hardly changed when comparing training and non-training Whippets.https://doi.org/10.1038/s41598-023-45546-w |
| spellingShingle | Katarzyna Miazga Klaudia Kopczyńska Olga Szaluś-Jordanow Agata Moroz-Fik Jacek Wilczak Karolina Barszcz Anna Cywińska Metabolomic analysis indicated changes in triacylglycerols’ levels as a result of training in Whippet dogs Scientific Reports |
| title | Metabolomic analysis indicated changes in triacylglycerols’ levels as a result of training in Whippet dogs |
| title_full | Metabolomic analysis indicated changes in triacylglycerols’ levels as a result of training in Whippet dogs |
| title_fullStr | Metabolomic analysis indicated changes in triacylglycerols’ levels as a result of training in Whippet dogs |
| title_full_unstemmed | Metabolomic analysis indicated changes in triacylglycerols’ levels as a result of training in Whippet dogs |
| title_short | Metabolomic analysis indicated changes in triacylglycerols’ levels as a result of training in Whippet dogs |
| title_sort | metabolomic analysis indicated changes in triacylglycerols levels as a result of training in whippet dogs |
| url | https://doi.org/10.1038/s41598-023-45546-w |
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