Performance characteristics of an antibody-based multiplex kit for determining recent HIV-1 infection.
The availability of reliable laboratory methods for determining recent HIV infection is vital for accurate estimation of population-based incidence. The mean duration of recent infection (MDRI) and false recent rate (FRR) are critical parameters for HIV incidence assays, as they impact HIV incidence...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5417525?pdf=render |
| _version_ | 1819079734100754432 |
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| author | Kelly A Curtis Debra L Hanson Krystin Ambrose Price S Michele Owen |
| author_facet | Kelly A Curtis Debra L Hanson Krystin Ambrose Price S Michele Owen |
| author_sort | Kelly A Curtis |
| collection | DOAJ |
| description | The availability of reliable laboratory methods for determining recent HIV infection is vital for accurate estimation of population-based incidence. The mean duration of recent infection (MDRI) and false recent rate (FRR) are critical parameters for HIV incidence assays, as they impact HIV incidence estimates and provide a measure of assay performance. The HIV-1 Multiplex assay is an in-house developed, magnetic bead-based assay that measures virus-specific antibody levels and avidity to multiple analytes. To ensure quality control and to facilitate transfer of the assay to external laboratories or testing facilities, the in-house assay has been adapted and produced in kit form. Here, we describe the performance characteristics of the multiplex kit and demonstrate the stability of the kit components over a one-year period. Two statistical methods were employed to estimate the MDRI of the individual analytes and five different algorithms, combining multiple analyte values. The MDRI estimates for the individual analytes and five algorithms were all between 200 and 300 days post-seroconversion, with no notable difference between the two statistical approaches. All five algorithms exhibited a 0% FRR with specimens from long-term, subtype B HIV-1-infected individuals. The assay parameters described in this study provide the necessary tools to implement the HIV-1 multiplex assay and improves the utility of the assay for field use. |
| first_indexed | 2024-12-21T19:33:41Z |
| format | Article |
| id | doaj.art-a2008554ab8b4b0c9aa5efe09907af76 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T19:33:41Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a2008554ab8b4b0c9aa5efe09907af762022-12-21T18:52:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01125e017659310.1371/journal.pone.0176593Performance characteristics of an antibody-based multiplex kit for determining recent HIV-1 infection.Kelly A CurtisDebra L HansonKrystin Ambrose PriceS Michele OwenThe availability of reliable laboratory methods for determining recent HIV infection is vital for accurate estimation of population-based incidence. The mean duration of recent infection (MDRI) and false recent rate (FRR) are critical parameters for HIV incidence assays, as they impact HIV incidence estimates and provide a measure of assay performance. The HIV-1 Multiplex assay is an in-house developed, magnetic bead-based assay that measures virus-specific antibody levels and avidity to multiple analytes. To ensure quality control and to facilitate transfer of the assay to external laboratories or testing facilities, the in-house assay has been adapted and produced in kit form. Here, we describe the performance characteristics of the multiplex kit and demonstrate the stability of the kit components over a one-year period. Two statistical methods were employed to estimate the MDRI of the individual analytes and five different algorithms, combining multiple analyte values. The MDRI estimates for the individual analytes and five algorithms were all between 200 and 300 days post-seroconversion, with no notable difference between the two statistical approaches. All five algorithms exhibited a 0% FRR with specimens from long-term, subtype B HIV-1-infected individuals. The assay parameters described in this study provide the necessary tools to implement the HIV-1 multiplex assay and improves the utility of the assay for field use.http://europepmc.org/articles/PMC5417525?pdf=render |
| spellingShingle | Kelly A Curtis Debra L Hanson Krystin Ambrose Price S Michele Owen Performance characteristics of an antibody-based multiplex kit for determining recent HIV-1 infection. PLoS ONE |
| title | Performance characteristics of an antibody-based multiplex kit for determining recent HIV-1 infection. |
| title_full | Performance characteristics of an antibody-based multiplex kit for determining recent HIV-1 infection. |
| title_fullStr | Performance characteristics of an antibody-based multiplex kit for determining recent HIV-1 infection. |
| title_full_unstemmed | Performance characteristics of an antibody-based multiplex kit for determining recent HIV-1 infection. |
| title_short | Performance characteristics of an antibody-based multiplex kit for determining recent HIV-1 infection. |
| title_sort | performance characteristics of an antibody based multiplex kit for determining recent hiv 1 infection |
| url | http://europepmc.org/articles/PMC5417525?pdf=render |
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