Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysis

Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysis

Summary: Here, we present a validated workflow to isolate sufficient viable single ovary cells from a single mouse without the need to pool from several mice. We provide steps essential for estrous staging, ovary harvesting and dissociation, ovary cell staining, data collection, and analysis. Our ap...

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Bibliographic Details
Main Authors: Enitome E. Bafor, Toni Martin, Jeffrey Carrell, Megan Karwan, Adrienne E. Kimmel, Uyi.G. Omogiade, Michael Sanford, Howard A. Young, Julio C. Valencia
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
Flow Cytometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723006779

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