Antibacterial Efficacy of Manuka Honey-Doped Chitosan-Gelatin Cryogel and Hydrogel Scaffolds in Reducing Infection
Honey has been used for centuries to reduce bacterial infection; Manuka honey (MH) possesses an additional antibacterial agent, Unique Manuka Factor (UMF). However, MH’s physical properties challenge delivery to the wound site. Tissue-engineered scaffolds (cryogels/hydrogels) provide a potential veh...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/9/11/877 |
| _version_ | 1797459207774011392 |
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| author | Karina Mitchell Sreejith S. Panicker Calista L. Adler George A. O’Toole Katherine R. Hixon |
| author_facet | Karina Mitchell Sreejith S. Panicker Calista L. Adler George A. O’Toole Katherine R. Hixon |
| author_sort | Karina Mitchell |
| collection | DOAJ |
| description | Honey has been used for centuries to reduce bacterial infection; Manuka honey (MH) possesses an additional antibacterial agent, Unique Manuka Factor (UMF). However, MH’s physical properties challenge delivery to the wound site. Tissue-engineered scaffolds (cryogels/hydrogels) provide a potential vehicle for MH delivery, but effects on bacterial clearance and biofilm formation demand further examination. MH (0, 1, 5, or 10%) was incorporated into both chitosan-gelatin (1:4 ratio; 4%) cryogels and hydrogels. To assess physical changes, all scaffolds were imaged with scanning electron microscopy and subjected to swell testing to quantify pore size and rehydration potential, respectively. As MH concentration increased, both pore size and scaffold swelling capacity decreased. Both bacterial clearance and biofilm formation were also assessed, along with cellular infiltration. Bacterial clearance testing with <i>S. aureus</i> demonstrated that MH cryogels are superior to 0% control, indicating the potential to perform well against Gram-positive bacteria. However, higher concentrations of MH resulted in cell death over time. These results support our hypothesis that MH release from 5% cryogels would induce reduced viability for four bacteria species without compromising scaffold properties. These outcomes assist in the development of a standard of practice for incorporating MH into scaffolds and the evaluation of biofilm reduction. |
| first_indexed | 2024-03-09T16:49:08Z |
| format | Article |
| id | doaj.art-a21021b87ed94b9d858343fbf6fd7114 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T16:49:08Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-a21021b87ed94b9d858343fbf6fd71142023-11-24T14:43:22ZengMDPI AGGels2310-28612023-11-0191187710.3390/gels9110877Antibacterial Efficacy of Manuka Honey-Doped Chitosan-Gelatin Cryogel and Hydrogel Scaffolds in Reducing InfectionKarina Mitchell0Sreejith S. Panicker1Calista L. Adler2George A. O’Toole3Katherine R. Hixon4Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USAThayer School of Engineering, Dartmouth College, Hanover, NH 03755, USAThayer School of Engineering, Dartmouth College, Hanover, NH 03755, USAGeisel School of Medicine at Dartmouth, Hanover, NH 03755, USAThayer School of Engineering, Dartmouth College, Hanover, NH 03755, USAHoney has been used for centuries to reduce bacterial infection; Manuka honey (MH) possesses an additional antibacterial agent, Unique Manuka Factor (UMF). However, MH’s physical properties challenge delivery to the wound site. Tissue-engineered scaffolds (cryogels/hydrogels) provide a potential vehicle for MH delivery, but effects on bacterial clearance and biofilm formation demand further examination. MH (0, 1, 5, or 10%) was incorporated into both chitosan-gelatin (1:4 ratio; 4%) cryogels and hydrogels. To assess physical changes, all scaffolds were imaged with scanning electron microscopy and subjected to swell testing to quantify pore size and rehydration potential, respectively. As MH concentration increased, both pore size and scaffold swelling capacity decreased. Both bacterial clearance and biofilm formation were also assessed, along with cellular infiltration. Bacterial clearance testing with <i>S. aureus</i> demonstrated that MH cryogels are superior to 0% control, indicating the potential to perform well against Gram-positive bacteria. However, higher concentrations of MH resulted in cell death over time. These results support our hypothesis that MH release from 5% cryogels would induce reduced viability for four bacteria species without compromising scaffold properties. These outcomes assist in the development of a standard of practice for incorporating MH into scaffolds and the evaluation of biofilm reduction.https://www.mdpi.com/2310-2861/9/11/877cryogelhydrogelscaffoldsManuka honeyinfectionantibacterial |
| spellingShingle | Karina Mitchell Sreejith S. Panicker Calista L. Adler George A. O’Toole Katherine R. Hixon Antibacterial Efficacy of Manuka Honey-Doped Chitosan-Gelatin Cryogel and Hydrogel Scaffolds in Reducing Infection Gels cryogel hydrogel scaffolds Manuka honey infection antibacterial |
| title | Antibacterial Efficacy of Manuka Honey-Doped Chitosan-Gelatin Cryogel and Hydrogel Scaffolds in Reducing Infection |
| title_full | Antibacterial Efficacy of Manuka Honey-Doped Chitosan-Gelatin Cryogel and Hydrogel Scaffolds in Reducing Infection |
| title_fullStr | Antibacterial Efficacy of Manuka Honey-Doped Chitosan-Gelatin Cryogel and Hydrogel Scaffolds in Reducing Infection |
| title_full_unstemmed | Antibacterial Efficacy of Manuka Honey-Doped Chitosan-Gelatin Cryogel and Hydrogel Scaffolds in Reducing Infection |
| title_short | Antibacterial Efficacy of Manuka Honey-Doped Chitosan-Gelatin Cryogel and Hydrogel Scaffolds in Reducing Infection |
| title_sort | antibacterial efficacy of manuka honey doped chitosan gelatin cryogel and hydrogel scaffolds in reducing infection |
| topic | cryogel hydrogel scaffolds Manuka honey infection antibacterial |
| url | https://www.mdpi.com/2310-2861/9/11/877 |
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