Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform
Introduction: Ammonia is a metabolite of protein catabolism that, when elevated, may be toxic for tissues, especially for the central nervous system. Elevated ammonia in blood is an indicator and a prognostic factor for hepatic and kidney disease or inherited metabolic disorders in nitrogen metaboli...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Practical Laboratory Medicine |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551718300349 |
| _version_ | 1811337588390232064 |
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| author | J. Kaplon J.J. de Groot J.P. van Straalen M. Heckman J.C. Fischer |
| author_facet | J. Kaplon J.J. de Groot J.P. van Straalen M. Heckman J.C. Fischer |
| author_sort | J. Kaplon |
| collection | DOAJ |
| description | Introduction: Ammonia is a metabolite of protein catabolism that, when elevated, may be toxic for tissues, especially for the central nervous system. Elevated ammonia in blood is an indicator and a prognostic factor for hepatic and kidney disease or inherited metabolic disorders in nitrogen metabolism. The accuracy of ammonia determination is influenced by sampling condition, handling, storage and assay itself. Our and other laboratories have been experiencing high frequencies sample error flags while measuring ammonia with glutamate dehydrogenase method on Roche Cobas 8000 platform. To reduce the number of error flags we adapted Roche NH3L protocol by incorporation of an additional onboard routine step for sample pre-dilution. Material and methods: The AMC NH3L is an adaptation of Roche protocol that uses four fold pre-dilution of the sample in the rerun prior to the analysis. It was assessed for 1.occurrence of absorbance error flags, 2.precision, 3.correlation with Roche method and 4.interference by hemolysis, icterus and lipemia. Results: The AMC NH3L adaptation demonstrates acceptable within-run and total precision. Comparison studies show no differences between the Roche rerun application and AMC NH3L adaptation. The AMC NH3L adaptation solves 78% of absorbance errors and for samples with high ammonia concentration is less affected by interferences from icterus and hemolysis than the Roche rerun application. Conclusion: The AMC NH3L adaptation is less prone to instrument error flags and for samples with high ammonia concentration, is more robust to endogenous interferences. The AMC NH3L adaptation is viable alternative to the Roche protocol for the ammonia measurement. Keywords: Ammonia, Roche automated platform, Absorbance error |
| first_indexed | 2024-04-13T17:57:14Z |
| format | Article |
| id | doaj.art-a2108321cd024e4496c9746cd67ea16e |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-04-13T17:57:14Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-a2108321cd024e4496c9746cd67ea16e2022-12-22T02:36:27ZengElsevierPractical Laboratory Medicine2352-55172019-01-0113Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platformJ. Kaplon0J.J. de Groot1J.P. van Straalen2M. Heckman3J.C. Fischer4Correspondence to: Academic Medical Center, Department of Clinical Chemistry, Postbus 22660, 1100 DD Amsterdam, the Netherlands.; Department of Clinical Chemistry, Academic Medical Center, Amsterdam, the NetherlandsDepartment of Clinical Chemistry, Academic Medical Center, Amsterdam, the NetherlandsDepartment of Clinical Chemistry, Academic Medical Center, Amsterdam, the NetherlandsDepartment of Clinical Chemistry, Academic Medical Center, Amsterdam, the NetherlandsDepartment of Clinical Chemistry, Academic Medical Center, Amsterdam, the NetherlandsIntroduction: Ammonia is a metabolite of protein catabolism that, when elevated, may be toxic for tissues, especially for the central nervous system. Elevated ammonia in blood is an indicator and a prognostic factor for hepatic and kidney disease or inherited metabolic disorders in nitrogen metabolism. The accuracy of ammonia determination is influenced by sampling condition, handling, storage and assay itself. Our and other laboratories have been experiencing high frequencies sample error flags while measuring ammonia with glutamate dehydrogenase method on Roche Cobas 8000 platform. To reduce the number of error flags we adapted Roche NH3L protocol by incorporation of an additional onboard routine step for sample pre-dilution. Material and methods: The AMC NH3L is an adaptation of Roche protocol that uses four fold pre-dilution of the sample in the rerun prior to the analysis. It was assessed for 1.occurrence of absorbance error flags, 2.precision, 3.correlation with Roche method and 4.interference by hemolysis, icterus and lipemia. Results: The AMC NH3L adaptation demonstrates acceptable within-run and total precision. Comparison studies show no differences between the Roche rerun application and AMC NH3L adaptation. The AMC NH3L adaptation solves 78% of absorbance errors and for samples with high ammonia concentration is less affected by interferences from icterus and hemolysis than the Roche rerun application. Conclusion: The AMC NH3L adaptation is less prone to instrument error flags and for samples with high ammonia concentration, is more robust to endogenous interferences. The AMC NH3L adaptation is viable alternative to the Roche protocol for the ammonia measurement. Keywords: Ammonia, Roche automated platform, Absorbance errorhttp://www.sciencedirect.com/science/article/pii/S2352551718300349 |
| spellingShingle | J. Kaplon J.J. de Groot J.P. van Straalen M. Heckman J.C. Fischer Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform Practical Laboratory Medicine |
| title | Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform |
| title_full | Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform |
| title_fullStr | Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform |
| title_full_unstemmed | Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform |
| title_short | Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform |
| title_sort | improved assay protocol for measurement of ammonia on the roche cobas 8000 automated platform |
| url | http://www.sciencedirect.com/science/article/pii/S2352551718300349 |
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