Prospects for Using Atomic Power Microscopic Methods in Reanimatology

Objective: to obtain an image of pores in the membrane of an erythrocyte after its electroporation, by using an atomic power microscope (APM), to evaluate the parameters of the obtained pores, and to discuss the prospects for using an APM in reanimatology. Human whole venous blood was exposed to a p...

Full description

Bibliographic Details
Main Authors: V. V. Moroz, A. M. Chernysh, I. V. Yaminsky, Ye. K. Kozlova, G. A. Kiselev, A. S. Filonov, M. S. Bogushevich, O. Ye. Gudkova
Format: Article
Language:English
Published: Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia 2008-08-01
Series:Общая реаниматология
Subjects:
Online Access:https://www.reanimatology.com/rmt/article/view/711
_version_ 1797874357982199808
author V. V. Moroz
A. M. Chernysh
I. V. Yaminsky
Ye. K. Kozlova
G. A. Kiselev
A. S. Filonov
M. S. Bogushevich
O. Ye. Gudkova
author_facet V. V. Moroz
A. M. Chernysh
I. V. Yaminsky
Ye. K. Kozlova
G. A. Kiselev
A. S. Filonov
M. S. Bogushevich
O. Ye. Gudkova
author_sort V. V. Moroz
collection DOAJ
description Objective: to obtain an image of pores in the membrane of an erythrocyte after its electroporation, by using an atomic power microscope (APM), to evaluate the parameters of the obtained pores, and to discuss the prospects for using an APM in reanimatology. Human whole venous blood was exposed to a pulsed electric field, by inducing erythrocytic membrane elec-troporation. Blood smears were obtained by the standard method. Images of the form and surface of individual erythrocytes and their enlarged fragments were obtained by means of a Femto Scan APM. The diameters, depths, and configuration of some pores were analyzed. This example and others were used to show a wide field and prospects for using an APM in reanimatology.
first_indexed 2024-04-10T01:30:43Z
format Article
id doaj.art-a21506430422416e983e3004e20a1f87
institution Directory Open Access Journal
issn 1813-9779
2411-7110
language English
last_indexed 2024-04-10T01:30:43Z
publishDate 2008-08-01
publisher Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
record_format Article
series Общая реаниматология
spelling doaj.art-a21506430422416e983e3004e20a1f872023-03-13T09:32:48ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102008-08-014410.15360/1813-9779-2008-4-51711Prospects for Using Atomic Power Microscopic Methods in ReanimatologyV. V. MorozA. M. ChernyshI. V. YaminskyYe. K. KozlovaG. A. KiselevA. S. FilonovM. S. BogushevichO. Ye. GudkovaObjective: to obtain an image of pores in the membrane of an erythrocyte after its electroporation, by using an atomic power microscope (APM), to evaluate the parameters of the obtained pores, and to discuss the prospects for using an APM in reanimatology. Human whole venous blood was exposed to a pulsed electric field, by inducing erythrocytic membrane elec-troporation. Blood smears were obtained by the standard method. Images of the form and surface of individual erythrocytes and their enlarged fragments were obtained by means of a Femto Scan APM. The diameters, depths, and configuration of some pores were analyzed. This example and others were used to show a wide field and prospects for using an APM in reanimatology.https://www.reanimatology.com/rmt/article/view/711атомный силовой микроскопэлектропорация мембрананализ пор.
spellingShingle V. V. Moroz
A. M. Chernysh
I. V. Yaminsky
Ye. K. Kozlova
G. A. Kiselev
A. S. Filonov
M. S. Bogushevich
O. Ye. Gudkova
Prospects for Using Atomic Power Microscopic Methods in Reanimatology
Общая реаниматология
атомный силовой микроскоп
электропорация мембран
анализ пор.
title Prospects for Using Atomic Power Microscopic Methods in Reanimatology
title_full Prospects for Using Atomic Power Microscopic Methods in Reanimatology
title_fullStr Prospects for Using Atomic Power Microscopic Methods in Reanimatology
title_full_unstemmed Prospects for Using Atomic Power Microscopic Methods in Reanimatology
title_short Prospects for Using Atomic Power Microscopic Methods in Reanimatology
title_sort prospects for using atomic power microscopic methods in reanimatology
topic атомный силовой микроскоп
электропорация мембран
анализ пор.
url https://www.reanimatology.com/rmt/article/view/711
work_keys_str_mv AT vvmoroz prospectsforusingatomicpowermicroscopicmethodsinreanimatology
AT amchernysh prospectsforusingatomicpowermicroscopicmethodsinreanimatology
AT ivyaminsky prospectsforusingatomicpowermicroscopicmethodsinreanimatology
AT yekkozlova prospectsforusingatomicpowermicroscopicmethodsinreanimatology
AT gakiselev prospectsforusingatomicpowermicroscopicmethodsinreanimatology
AT asfilonov prospectsforusingatomicpowermicroscopicmethodsinreanimatology
AT msbogushevich prospectsforusingatomicpowermicroscopicmethodsinreanimatology
AT oyegudkova prospectsforusingatomicpowermicroscopicmethodsinreanimatology