Transgenic Expression of Synthetic Coat Protein and Synthetic Replication Associated Protein Produces Mild Symptoms and Reduce Begomovirus-Betasatellite Accumulation in Nicotiana benthamiana
Cotton (Gossypium hirsutum L.) is the most essential fiber crop and is widely cultivated within the tropical regions of the world. It has a major impact on socio-economical get-together in Pakistan. Unfortunately, it has been tormented by cotton leaf curl disease (CLCuD) that is produced by distinct...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Agronomy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fagro.2021.676820/full |
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| author | Ghulam Rasool Ghulam Rasool Sumaira Yousaf Sumaira Yousaf Um e Ammara Um e Ammara Adnan Iqbal Muhammad Saeed Muhammad Saeed Imran Amin Shahid Mansoor |
| author_facet | Ghulam Rasool Ghulam Rasool Sumaira Yousaf Sumaira Yousaf Um e Ammara Um e Ammara Adnan Iqbal Muhammad Saeed Muhammad Saeed Imran Amin Shahid Mansoor |
| author_sort | Ghulam Rasool |
| collection | DOAJ |
| description | Cotton (Gossypium hirsutum L.) is the most essential fiber crop and is widely cultivated within the tropical regions of the world. It has a major impact on socio-economical get-together in Pakistan. Unfortunately, it has been tormented by cotton leaf curl disease (CLCuD) that is produced by distinct species of begomoviruses (Family; Geminiviridae) like Cotton leaf curl Kokhran virus-Burewala strain (CLCuKoV-Bu) associated with specific betasatellite—Cotton leaf curl Multan betasatellite (CLCuMuB). Host resistance against CLCuD has not been achieved yet. The present study employed the use of synthetic genes considering the phenomenon of pathogen derived resistance (PDR). The coat protein (CP) and replication associated protein (Rep) genes sequence from CLCuKoV-Bu were codon optimized, synthesized and used to control CLCuKoV-Bu and associated betasatellite related to CLCuD. The Nicotiana benthamiana plants were Agrobacterium mediated transformed with synthetic CP (CPsyn) and synthetic Rep (Repsyn) under the control of Cauliflower mosaic virus 35S promoter. The transgenic plants harboring CPsyn and Repsyn genes were infiltrated with CLCuKoV-Bu alone and CLCuKoV-Bu- CLCuMuB inoculums showed relatively milder symptoms as compared with wild sorts and low virus concentration as checked by southern hybridization. The results confirmed that CPsyn, Repsyn genes may be utilized for the resistance of CLCuKoV-Bu and associated betasatellites related to CLCuD and PDR mechanism may also be developed for the control of CLCuD. |
| first_indexed | 2024-12-24T03:08:55Z |
| format | Article |
| id | doaj.art-a216a347a84841ae9933bf7af8f9f585 |
| institution | Directory Open Access Journal |
| issn | 2673-3218 |
| language | English |
| last_indexed | 2024-12-24T03:08:55Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Agronomy |
| spelling | doaj.art-a216a347a84841ae9933bf7af8f9f5852022-12-21T17:17:53ZengFrontiers Media S.A.Frontiers in Agronomy2673-32182021-10-01310.3389/fagro.2021.676820676820Transgenic Expression of Synthetic Coat Protein and Synthetic Replication Associated Protein Produces Mild Symptoms and Reduce Begomovirus-Betasatellite Accumulation in Nicotiana benthamianaGhulam Rasool0Ghulam Rasool1Sumaira Yousaf2Sumaira Yousaf3Um e Ammara4Um e Ammara5Adnan Iqbal6Muhammad Saeed7Muhammad Saeed8Imran Amin9Shahid Mansoor10Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, PakistanNIBGE College PIEAS, Pakistan Institute of Engineering and Applied Sciences, Islamabad, PakistanAgricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, PakistanPlant Protection Division, Nuclear Institute for Agriculture and Biology, Faisalabad, PakistanAgricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, PakistanDepartment of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, OmanInstitute of Molecular Biology and Biotechnology (IMBB), Centre for Research in Molecular Medicine (CRIMM), University of Lahore, Lahore, PakistanAgricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, PakistanTechnische Universität Kaiserslautern Abteilung Phytopathologie, Fachbereich Biologie, Kaiserslautern, GermanyAgricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, PakistanAgricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, PakistanCotton (Gossypium hirsutum L.) is the most essential fiber crop and is widely cultivated within the tropical regions of the world. It has a major impact on socio-economical get-together in Pakistan. Unfortunately, it has been tormented by cotton leaf curl disease (CLCuD) that is produced by distinct species of begomoviruses (Family; Geminiviridae) like Cotton leaf curl Kokhran virus-Burewala strain (CLCuKoV-Bu) associated with specific betasatellite—Cotton leaf curl Multan betasatellite (CLCuMuB). Host resistance against CLCuD has not been achieved yet. The present study employed the use of synthetic genes considering the phenomenon of pathogen derived resistance (PDR). The coat protein (CP) and replication associated protein (Rep) genes sequence from CLCuKoV-Bu were codon optimized, synthesized and used to control CLCuKoV-Bu and associated betasatellite related to CLCuD. The Nicotiana benthamiana plants were Agrobacterium mediated transformed with synthetic CP (CPsyn) and synthetic Rep (Repsyn) under the control of Cauliflower mosaic virus 35S promoter. The transgenic plants harboring CPsyn and Repsyn genes were infiltrated with CLCuKoV-Bu alone and CLCuKoV-Bu- CLCuMuB inoculums showed relatively milder symptoms as compared with wild sorts and low virus concentration as checked by southern hybridization. The results confirmed that CPsyn, Repsyn genes may be utilized for the resistance of CLCuKoV-Bu and associated betasatellites related to CLCuD and PDR mechanism may also be developed for the control of CLCuD.https://www.frontiersin.org/articles/10.3389/fagro.2021.676820/fullcotton leaf curl diseasecotton leaf curl Kokhran virus-Burewala straincotton leaf curl Multan betasatellitesynthetic coat proteinsynthetic replication associated proteintransgenic resistance |
| spellingShingle | Ghulam Rasool Ghulam Rasool Sumaira Yousaf Sumaira Yousaf Um e Ammara Um e Ammara Adnan Iqbal Muhammad Saeed Muhammad Saeed Imran Amin Shahid Mansoor Transgenic Expression of Synthetic Coat Protein and Synthetic Replication Associated Protein Produces Mild Symptoms and Reduce Begomovirus-Betasatellite Accumulation in Nicotiana benthamiana Frontiers in Agronomy cotton leaf curl disease cotton leaf curl Kokhran virus-Burewala strain cotton leaf curl Multan betasatellite synthetic coat protein synthetic replication associated protein transgenic resistance |
| title | Transgenic Expression of Synthetic Coat Protein and Synthetic Replication Associated Protein Produces Mild Symptoms and Reduce Begomovirus-Betasatellite Accumulation in Nicotiana benthamiana |
| title_full | Transgenic Expression of Synthetic Coat Protein and Synthetic Replication Associated Protein Produces Mild Symptoms and Reduce Begomovirus-Betasatellite Accumulation in Nicotiana benthamiana |
| title_fullStr | Transgenic Expression of Synthetic Coat Protein and Synthetic Replication Associated Protein Produces Mild Symptoms and Reduce Begomovirus-Betasatellite Accumulation in Nicotiana benthamiana |
| title_full_unstemmed | Transgenic Expression of Synthetic Coat Protein and Synthetic Replication Associated Protein Produces Mild Symptoms and Reduce Begomovirus-Betasatellite Accumulation in Nicotiana benthamiana |
| title_short | Transgenic Expression of Synthetic Coat Protein and Synthetic Replication Associated Protein Produces Mild Symptoms and Reduce Begomovirus-Betasatellite Accumulation in Nicotiana benthamiana |
| title_sort | transgenic expression of synthetic coat protein and synthetic replication associated protein produces mild symptoms and reduce begomovirus betasatellite accumulation in nicotiana benthamiana |
| topic | cotton leaf curl disease cotton leaf curl Kokhran virus-Burewala strain cotton leaf curl Multan betasatellite synthetic coat protein synthetic replication associated protein transgenic resistance |
| url | https://www.frontiersin.org/articles/10.3389/fagro.2021.676820/full |
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