Fungal Chitin Reduces Platelet Activation Mediated via TLR8 Stimulation
Platelets play an important role in the innate immune response. During candidaemia, circulating fungal polysaccharides, including chitin, are released into the bloodstream and can interact with platelets and induce modulation of platelet activities. However, the role of circulating chitin in platele...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2019.00383/full |
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| author | Jordan Leroy Jordan Leroy Jordan Leroy Clovis Bortolus Clovis Bortolus Karine Lecointe Karine Lecointe Melissa Parny Melissa Parny Rogatien Charlet Rogatien Charlet Boualem Sendid Boualem Sendid Boualem Sendid Samir Jawhara Samir Jawhara |
| author_facet | Jordan Leroy Jordan Leroy Jordan Leroy Clovis Bortolus Clovis Bortolus Karine Lecointe Karine Lecointe Melissa Parny Melissa Parny Rogatien Charlet Rogatien Charlet Boualem Sendid Boualem Sendid Boualem Sendid Samir Jawhara Samir Jawhara |
| author_sort | Jordan Leroy |
| collection | DOAJ |
| description | Platelets play an important role in the innate immune response. During candidaemia, circulating fungal polysaccharides, including chitin, are released into the bloodstream and can interact with platelets and induce modulation of platelet activities. However, the role of circulating chitin in platelet modulation has not been investigated. The aims of the present study were to assess the effect of fungal chitin on activation, adhesion, aggregation and receptor expression of platelets and their impact on the host defense against Candida albicans. Platelets pre-treated with different concentrations of chitin (10–400 μg/mL) extracted from C. albicans were analyzed in terms of activation, Toll-like receptor (TLR) expression, aggregation and adhesion to C. albicans. Chitin treatment reduced platelet adhesion to C. albicans and neutrophils. P-selectin expression was significantly decreased in platelets challenged with chitin. Aggregation and intracellular Ca2+ influx were also decreased in platelets. TLR8 mRNA and proteins were expressed in platelets pre-treated with chitin when compared to untreated platelets. Overall, chitin purified from C. albicans reduced the adhesion, activation and aggregation of platelets mediated via TLR8 stimulation by decreasing intracellular Ca2+ influx and P-selectin expression. |
| first_indexed | 2024-12-11T13:31:24Z |
| format | Article |
| id | doaj.art-a217cd8f32c649b98f6f1bb9c6fac9e3 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-11T13:31:24Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-a217cd8f32c649b98f6f1bb9c6fac9e32022-12-22T01:05:16ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882019-11-01910.3389/fcimb.2019.00383493779Fungal Chitin Reduces Platelet Activation Mediated via TLR8 StimulationJordan Leroy0Jordan Leroy1Jordan Leroy2Clovis Bortolus3Clovis Bortolus4Karine Lecointe5Karine Lecointe6Melissa Parny7Melissa Parny8Rogatien Charlet9Rogatien Charlet10Boualem Sendid11Boualem Sendid12Boualem Sendid13Samir Jawhara14Samir Jawhara15INSERM, U995/Team2, Lille, FranceLille Inflammation Research International Centre, University of Lille, U995-LIRIC, Lille, FranceService de Parasitologie Mycologie, Pôle de Biologie Pathologie Génétique, CHU Lille, Lille, FranceINSERM, U995/Team2, Lille, FranceLille Inflammation Research International Centre, University of Lille, U995-LIRIC, Lille, FranceINSERM, U995/Team2, Lille, FranceLille Inflammation Research International Centre, University of Lille, U995-LIRIC, Lille, FranceINSERM, U995/Team2, Lille, FranceLille Inflammation Research International Centre, University of Lille, U995-LIRIC, Lille, FranceINSERM, U995/Team2, Lille, FranceLille Inflammation Research International Centre, University of Lille, U995-LIRIC, Lille, FranceINSERM, U995/Team2, Lille, FranceLille Inflammation Research International Centre, University of Lille, U995-LIRIC, Lille, FranceService de Parasitologie Mycologie, Pôle de Biologie Pathologie Génétique, CHU Lille, Lille, FranceINSERM, U995/Team2, Lille, FranceLille Inflammation Research International Centre, University of Lille, U995-LIRIC, Lille, FrancePlatelets play an important role in the innate immune response. During candidaemia, circulating fungal polysaccharides, including chitin, are released into the bloodstream and can interact with platelets and induce modulation of platelet activities. However, the role of circulating chitin in platelet modulation has not been investigated. The aims of the present study were to assess the effect of fungal chitin on activation, adhesion, aggregation and receptor expression of platelets and their impact on the host defense against Candida albicans. Platelets pre-treated with different concentrations of chitin (10–400 μg/mL) extracted from C. albicans were analyzed in terms of activation, Toll-like receptor (TLR) expression, aggregation and adhesion to C. albicans. Chitin treatment reduced platelet adhesion to C. albicans and neutrophils. P-selectin expression was significantly decreased in platelets challenged with chitin. Aggregation and intracellular Ca2+ influx were also decreased in platelets. TLR8 mRNA and proteins were expressed in platelets pre-treated with chitin when compared to untreated platelets. Overall, chitin purified from C. albicans reduced the adhesion, activation and aggregation of platelets mediated via TLR8 stimulation by decreasing intracellular Ca2+ influx and P-selectin expression.https://www.frontiersin.org/article/10.3389/fcimb.2019.00383/fullCandida albicanschitinplateletsneutrophilsToll-like receptorP-selectin |
| spellingShingle | Jordan Leroy Jordan Leroy Jordan Leroy Clovis Bortolus Clovis Bortolus Karine Lecointe Karine Lecointe Melissa Parny Melissa Parny Rogatien Charlet Rogatien Charlet Boualem Sendid Boualem Sendid Boualem Sendid Samir Jawhara Samir Jawhara Fungal Chitin Reduces Platelet Activation Mediated via TLR8 Stimulation Frontiers in Cellular and Infection Microbiology Candida albicans chitin platelets neutrophils Toll-like receptor P-selectin |
| title | Fungal Chitin Reduces Platelet Activation Mediated via TLR8 Stimulation |
| title_full | Fungal Chitin Reduces Platelet Activation Mediated via TLR8 Stimulation |
| title_fullStr | Fungal Chitin Reduces Platelet Activation Mediated via TLR8 Stimulation |
| title_full_unstemmed | Fungal Chitin Reduces Platelet Activation Mediated via TLR8 Stimulation |
| title_short | Fungal Chitin Reduces Platelet Activation Mediated via TLR8 Stimulation |
| title_sort | fungal chitin reduces platelet activation mediated via tlr8 stimulation |
| topic | Candida albicans chitin platelets neutrophils Toll-like receptor P-selectin |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2019.00383/full |
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