Comparing 3D ultrastructure of presynaptic and postsynaptic mitochondria
Serial-section electron microscopy such as FIB-SEM (focused ion beam scanning electron microscopy) has become an important tool for neuroscientists to trace the trajectories and global architecture of neural circuits in the brain, as well as to visualize the 3D ultrastructure of cellular organelles...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2019-08-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/8/8/bio044834 |
| _version_ | 1819070336247791616 |
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| author | Thomas Delgado Ronald S. Petralia David W. Freeman Miloslav Sedlacek Ya-Xian Wang Stephan D. Brenowitz Shu-Hsien Sheu Jeffrey W. Gu Dimitrios Kapogiannis Mark P. Mattson Pamela J. Yao |
| author_facet | Thomas Delgado Ronald S. Petralia David W. Freeman Miloslav Sedlacek Ya-Xian Wang Stephan D. Brenowitz Shu-Hsien Sheu Jeffrey W. Gu Dimitrios Kapogiannis Mark P. Mattson Pamela J. Yao |
| author_sort | Thomas Delgado |
| collection | DOAJ |
| description | Serial-section electron microscopy such as FIB-SEM (focused ion beam scanning electron microscopy) has become an important tool for neuroscientists to trace the trajectories and global architecture of neural circuits in the brain, as well as to visualize the 3D ultrastructure of cellular organelles in neurons. In this study, we examined 3D features of mitochondria in electron microscope images generated from serial sections of four regions of mouse brains: nucleus accumbens (NA), hippocampal CA1, somatosensory cortex and dorsal cochlear nucleus (DCN). We compared mitochondria in the presynaptic terminals to those in the postsynaptic/dendritic compartments, and we focused on the shape and size of mitochondria. A common feature of mitochondria among the four brain regions is that presynaptic mitochondria generally are small and short, and most of them do not extend beyond presynaptic terminals. In contrast, the majority of postsynaptic/dendritic mitochondria are large and many of them spread through significant portions of the dendrites. Comparing among the brain areas, the cerebral cortex and DCN have even larger postsynaptic/dendritic mitochondria than the NA and CA1. Our analysis reveals that mitochondria in neurons are differentially sized and arranged according to their subcellular locations, suggesting a spatial organizing principle of mitochondria at the synapse. |
| first_indexed | 2024-12-21T17:04:19Z |
| format | Article |
| id | doaj.art-a219570d5c1b48e69bca21380b0e4090 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-21T17:04:19Z |
| publishDate | 2019-08-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-a219570d5c1b48e69bca21380b0e40902022-12-21T18:56:34ZengThe Company of BiologistsBiology Open2046-63902019-08-018810.1242/bio.044834044834Comparing 3D ultrastructure of presynaptic and postsynaptic mitochondriaThomas Delgado0Ronald S. Petralia1David W. Freeman2Miloslav Sedlacek3Ya-Xian Wang4Stephan D. Brenowitz5Shu-Hsien Sheu6Jeffrey W. Gu7Dimitrios Kapogiannis8Mark P. Mattson9Pamela J. Yao10 Laboratory of Neurosciences, NIA/NIH, Baltimore, Maryland 21224, USA Advanced Imaging Core, NIDCD/NIH, Bethesda, Maryland 20892, USA Laboratory of Neurosciences, NIA/NIH, Baltimore, Maryland 21224, USA NIDCD/NIH, Bethesda, Maryland 20892, USA Advanced Imaging Core, NIDCD/NIH, Bethesda, Maryland 20892, USA NIDCD/NIH, Bethesda, Maryland 20892, USA Janelia Research Campus, Ashburn, Virginia 20147, USA Laboratory of Neurosciences, NIA/NIH, Baltimore, Maryland 21224, USA Laboratory of Neurosciences, NIA/NIH, Baltimore, Maryland 21224, USA Laboratory of Neurosciences, NIA/NIH, Baltimore, Maryland 21224, USA Laboratory of Neurosciences, NIA/NIH, Baltimore, Maryland 21224, USA Serial-section electron microscopy such as FIB-SEM (focused ion beam scanning electron microscopy) has become an important tool for neuroscientists to trace the trajectories and global architecture of neural circuits in the brain, as well as to visualize the 3D ultrastructure of cellular organelles in neurons. In this study, we examined 3D features of mitochondria in electron microscope images generated from serial sections of four regions of mouse brains: nucleus accumbens (NA), hippocampal CA1, somatosensory cortex and dorsal cochlear nucleus (DCN). We compared mitochondria in the presynaptic terminals to those in the postsynaptic/dendritic compartments, and we focused on the shape and size of mitochondria. A common feature of mitochondria among the four brain regions is that presynaptic mitochondria generally are small and short, and most of them do not extend beyond presynaptic terminals. In contrast, the majority of postsynaptic/dendritic mitochondria are large and many of them spread through significant portions of the dendrites. Comparing among the brain areas, the cerebral cortex and DCN have even larger postsynaptic/dendritic mitochondria than the NA and CA1. Our analysis reveals that mitochondria in neurons are differentially sized and arranged according to their subcellular locations, suggesting a spatial organizing principle of mitochondria at the synapse.http://bio.biologists.org/content/8/8/bio044834SynapseDendriteAxonFIB-SEM3D reconstruction |
| spellingShingle | Thomas Delgado Ronald S. Petralia David W. Freeman Miloslav Sedlacek Ya-Xian Wang Stephan D. Brenowitz Shu-Hsien Sheu Jeffrey W. Gu Dimitrios Kapogiannis Mark P. Mattson Pamela J. Yao Comparing 3D ultrastructure of presynaptic and postsynaptic mitochondria Biology Open Synapse Dendrite Axon FIB-SEM 3D reconstruction |
| title | Comparing 3D ultrastructure of presynaptic and postsynaptic mitochondria |
| title_full | Comparing 3D ultrastructure of presynaptic and postsynaptic mitochondria |
| title_fullStr | Comparing 3D ultrastructure of presynaptic and postsynaptic mitochondria |
| title_full_unstemmed | Comparing 3D ultrastructure of presynaptic and postsynaptic mitochondria |
| title_short | Comparing 3D ultrastructure of presynaptic and postsynaptic mitochondria |
| title_sort | comparing 3d ultrastructure of presynaptic and postsynaptic mitochondria |
| topic | Synapse Dendrite Axon FIB-SEM 3D reconstruction |
| url | http://bio.biologists.org/content/8/8/bio044834 |
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