Effects Of Triptolide On Tooth Movement And Root Resorption In Rats
Fan Yang,1,2 Xu Xia Wang,1,3 Dan Ma,1,2 Qun Cui,1,2 De Hua Zheng,1,2 Xiao Can Liu,1,2 Jun Zhang1,2 1Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University,...
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Dove Medical Press
2019-11-01
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| Series: | Drug Design, Development and Therapy |
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| Online Access: | https://www.dovepress.com/effects-of-triptolide-on-tooth-movement-and-root-resorption-in-rats-peer-reviewed-article-DDDT |
| _version_ | 1818603572954136576 |
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| author | Yang F Wang XX Ma D Cui Q Zheng DH Liu XC Zhang J |
| author_facet | Yang F Wang XX Ma D Cui Q Zheng DH Liu XC Zhang J |
| author_sort | Yang F |
| collection | DOAJ |
| description | Fan Yang,1,2 Xu Xia Wang,1,3 Dan Ma,1,2 Qun Cui,1,2 De Hua Zheng,1,2 Xiao Can Liu,1,2 Jun Zhang1,2 1Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China; 2Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China; 3Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of ChinaCorrespondence: Jun ZhangDepartment of Orthodontics, School of Stomatology, Shandong University, 44 Wenhua West Road, Jinan 250012, People’s Republic of ChinaTel +86 139 5310 9816Fax +86-531-88565657Email zhangj@sdu.edu.cnPurpose: The aim of this study was to investigate the effects of triptolide on the tooth movement and root resorption in rats during orthodontic treatment.Material and methods: A total of 48 male Wistar rats were divided into three groups of 16 each. The right maxillary first molars of rats were drawn mesially by closed coil nickel–titanium spring with a force of 50 g. The two experimental groups received intraperitoneal injections of triptolide for 14 days at a dose of 15 μg/kg/day and 30 μg/kg/day, respectively. The control group received vehicle injections. After 14 days, the rats were humanely killed. The amount of tooth movement was measured. Eight rats from each group were randomly chosen for analysis of the percentage of root resorption area by scanning electron microscopy. For the remaining eight rats in each group, the H&E staining, tartrate-resistant acid phosphatase (TRAP) staining and immunohistochemistry analysis were performed.Results: The amount of tooth movement and the ratio of root resorption area were significantly decreased in the triptolide-treated rats. The number of TRAP-positive cells was significantly lower in triptolide-treated groups. Moreover, the expression of nuclear factor kappa B ligand (RANKL) was reduced. In contrast, the expression of osteoprotegerin was significantly up-regulated. In the tension side, the expressions of runt-related transcription factor 2 and osteocalcin were significantly enhanced by triptolide injection.Conclusion: Triptolide injection could arrest orthodontic tooth movement and reduce root resorption in rats via inhibition of osteoclastogenesis. In addition, triptolide may exert a positive effect on osteoblastogenesis.Keywords: triptolide, osteoclast, scanning electron microscope, tooth movement, bone formation |
| first_indexed | 2024-12-16T13:25:19Z |
| format | Article |
| id | doaj.art-a219bd1db87644ab92964a7a24f59929 |
| institution | Directory Open Access Journal |
| issn | 1177-8881 |
| language | English |
| last_indexed | 2024-12-16T13:25:19Z |
| publishDate | 2019-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Drug Design, Development and Therapy |
| spelling | doaj.art-a219bd1db87644ab92964a7a24f599292022-12-21T22:30:14ZengDove Medical PressDrug Design, Development and Therapy1177-88812019-11-01Volume 133963397549976Effects Of Triptolide On Tooth Movement And Root Resorption In RatsYang FWang XXMa DCui QZheng DHLiu XCZhang JFan Yang,1,2 Xu Xia Wang,1,3 Dan Ma,1,2 Qun Cui,1,2 De Hua Zheng,1,2 Xiao Can Liu,1,2 Jun Zhang1,2 1Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China; 2Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China; 3Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of ChinaCorrespondence: Jun ZhangDepartment of Orthodontics, School of Stomatology, Shandong University, 44 Wenhua West Road, Jinan 250012, People’s Republic of ChinaTel +86 139 5310 9816Fax +86-531-88565657Email zhangj@sdu.edu.cnPurpose: The aim of this study was to investigate the effects of triptolide on the tooth movement and root resorption in rats during orthodontic treatment.Material and methods: A total of 48 male Wistar rats were divided into three groups of 16 each. The right maxillary first molars of rats were drawn mesially by closed coil nickel–titanium spring with a force of 50 g. The two experimental groups received intraperitoneal injections of triptolide for 14 days at a dose of 15 μg/kg/day and 30 μg/kg/day, respectively. The control group received vehicle injections. After 14 days, the rats were humanely killed. The amount of tooth movement was measured. Eight rats from each group were randomly chosen for analysis of the percentage of root resorption area by scanning electron microscopy. For the remaining eight rats in each group, the H&E staining, tartrate-resistant acid phosphatase (TRAP) staining and immunohistochemistry analysis were performed.Results: The amount of tooth movement and the ratio of root resorption area were significantly decreased in the triptolide-treated rats. The number of TRAP-positive cells was significantly lower in triptolide-treated groups. Moreover, the expression of nuclear factor kappa B ligand (RANKL) was reduced. In contrast, the expression of osteoprotegerin was significantly up-regulated. In the tension side, the expressions of runt-related transcription factor 2 and osteocalcin were significantly enhanced by triptolide injection.Conclusion: Triptolide injection could arrest orthodontic tooth movement and reduce root resorption in rats via inhibition of osteoclastogenesis. In addition, triptolide may exert a positive effect on osteoblastogenesis.Keywords: triptolide, osteoclast, scanning electron microscope, tooth movement, bone formationhttps://www.dovepress.com/effects-of-triptolide-on-tooth-movement-and-root-resorption-in-rats-peer-reviewed-article-DDDTtriptolideosteoclastscanning electron microspoetooth movementbone formation |
| spellingShingle | Yang F Wang XX Ma D Cui Q Zheng DH Liu XC Zhang J Effects Of Triptolide On Tooth Movement And Root Resorption In Rats Drug Design, Development and Therapy triptolide osteoclast scanning electron microspoe tooth movement bone formation |
| title | Effects Of Triptolide On Tooth Movement And Root Resorption In Rats |
| title_full | Effects Of Triptolide On Tooth Movement And Root Resorption In Rats |
| title_fullStr | Effects Of Triptolide On Tooth Movement And Root Resorption In Rats |
| title_full_unstemmed | Effects Of Triptolide On Tooth Movement And Root Resorption In Rats |
| title_short | Effects Of Triptolide On Tooth Movement And Root Resorption In Rats |
| title_sort | effects of triptolide on tooth movement and root resorption in rats |
| topic | triptolide osteoclast scanning electron microspoe tooth movement bone formation |
| url | https://www.dovepress.com/effects-of-triptolide-on-tooth-movement-and-root-resorption-in-rats-peer-reviewed-article-DDDT |
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