Genotypic and Phenotypic Characterization of MBL Genes in Pseudomonas aeruginosa Isolates from the Non-hospital Environment
The non-hospital environment particularly poultry farms and abattoirs are fast becoming reservoir channels for the transmission of antibiotic resistant bacteria including those that produce metallo beta-lactamases (MBLs). Foodproducing animal’s habouring multidrug resistant bacteria including those...
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Journal of Pure and Applied Microbiology
2018-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/genotypic-and-phenotypic-characterization-of-mbl-genes-in-pseudomonas-aeruginosa-isolates-from-the-non-hospital-environment/ |
| _version_ | 1818398321391173632 |
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| author | Chika Ejikeugwu Charles Esimone Ifeanyichukwu Iroha Peter Eze Malachy Ugwu Michael Adikwu |
| author_facet | Chika Ejikeugwu Charles Esimone Ifeanyichukwu Iroha Peter Eze Malachy Ugwu Michael Adikwu |
| author_sort | Chika Ejikeugwu |
| collection | DOAJ |
| description | The non-hospital environment particularly poultry farms and abattoirs are fast becoming reservoir channels for the
transmission of antibiotic resistant bacteria including those that produce metallo beta-lactamases (MBLs). Foodproducing animal’s habouring multidrug resistant bacteria including those that produce metallo-beta-lactamases
(MBLs) poses health risks to the human population. This study investigated the prevalence of blaIMP-1 and blaVIM-1
MBL genes in Pseudomonas aeruginosa isolates from food-producing animals by multiplex PCR technique. Anal
swab samples (n=120) were bacteriologically analyzed on cetrimide selective agar for the selective isolation of P.
aeruginosa isolates. Antibiogram was carried out as per the Clinical and Laboratory Standard Institute (CLSI) criteria.
The production of MBLs was detected phenotypically and genotypically using the modified Hodges test method
and multiplex PCR technique respectively. DNA products were run on 1.5 % agarose gel, and visualized using a UV
transilluminator at 260 nm. Data was analyzed statistically using SPSS version 23.0. Out of the 120 anal swab samples,
a total of 43 (35.8 %) isolates of P. aeruginosa was bacteriologically recovered. The P. aeruginosa isolates were found
to be resistant to ampicillin (88.4 %), cefotaxime (81.4 %), gentamicin (79.1 %), sulphamethoxazole-trimethoprim
(72.1 %), oxacillin (76.7 %), nitrofurantoin (76.7 %), meropenem (62.8 %), ofloxacin (67.4 %), imipenem (65.1 %)
and cloxacillin (69.8 %). MBL was phenotypically detected in 15 (34.9 %) isolates of P. aeruginosa. However, the
multiplex PCR technique significantly confirmed MBL production in only 12 (27.9) isolates of P. aeruginosa (p<0.001)
that harboured the blaIMP-1 MBL genes. The blaVIM-1 MBL genes were not detected in the MBL positive P. aeruginosa
phenotypes. The P. aeruginosa isolates that harboured the blaIMP-1 MBL genes were found to be multiply resistant.
This study reported for the first time the prevalence of blaIMP-1 MBL genes from P. aeruginosa isolates from anal
swab samples of food-producing animals in Abakaliki, Nigeria. The long-term exposure of food-producing animals
to antibiotics could cause accumulation of antibiotic resistance determinants in the gut microbiota of these animals. |
| first_indexed | 2024-12-14T07:02:56Z |
| format | Article |
| id | doaj.art-a21cd210c80244ee9b93ad66cf9315bd |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-14T07:02:56Z |
| publishDate | 2018-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-a21cd210c80244ee9b93ad66cf9315bd2022-12-21T23:12:21ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-12-011241877188510.22207/JPAM.12.4.23Genotypic and Phenotypic Characterization of MBL Genes in Pseudomonas aeruginosa Isolates from the Non-hospital EnvironmentChika Ejikeugwu0Charles Esimone1Ifeanyichukwu Iroha2Peter Eze3Malachy Ugwu4Michael Adikwu5Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B. 053, Ebonyi State, Nigeria. Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, P.M.B 5025, Anambra State, Nigeria.Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B. 053, Ebonyi State, Nigeria. Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, P.M.B 5025, Anambra State, Nigeria.Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, P.M.B 5025, Anambra State, Nigeria.Department of Pharmaceutics, Faculty of Pharmacy, University of Nigeria Nsukka (UNN), Nigeria.The non-hospital environment particularly poultry farms and abattoirs are fast becoming reservoir channels for the transmission of antibiotic resistant bacteria including those that produce metallo beta-lactamases (MBLs). Foodproducing animal’s habouring multidrug resistant bacteria including those that produce metallo-beta-lactamases (MBLs) poses health risks to the human population. This study investigated the prevalence of blaIMP-1 and blaVIM-1 MBL genes in Pseudomonas aeruginosa isolates from food-producing animals by multiplex PCR technique. Anal swab samples (n=120) were bacteriologically analyzed on cetrimide selective agar for the selective isolation of P. aeruginosa isolates. Antibiogram was carried out as per the Clinical and Laboratory Standard Institute (CLSI) criteria. The production of MBLs was detected phenotypically and genotypically using the modified Hodges test method and multiplex PCR technique respectively. DNA products were run on 1.5 % agarose gel, and visualized using a UV transilluminator at 260 nm. Data was analyzed statistically using SPSS version 23.0. Out of the 120 anal swab samples, a total of 43 (35.8 %) isolates of P. aeruginosa was bacteriologically recovered. The P. aeruginosa isolates were found to be resistant to ampicillin (88.4 %), cefotaxime (81.4 %), gentamicin (79.1 %), sulphamethoxazole-trimethoprim (72.1 %), oxacillin (76.7 %), nitrofurantoin (76.7 %), meropenem (62.8 %), ofloxacin (67.4 %), imipenem (65.1 %) and cloxacillin (69.8 %). MBL was phenotypically detected in 15 (34.9 %) isolates of P. aeruginosa. However, the multiplex PCR technique significantly confirmed MBL production in only 12 (27.9) isolates of P. aeruginosa (p<0.001) that harboured the blaIMP-1 MBL genes. The blaVIM-1 MBL genes were not detected in the MBL positive P. aeruginosa phenotypes. The P. aeruginosa isolates that harboured the blaIMP-1 MBL genes were found to be multiply resistant. This study reported for the first time the prevalence of blaIMP-1 MBL genes from P. aeruginosa isolates from anal swab samples of food-producing animals in Abakaliki, Nigeria. The long-term exposure of food-producing animals to antibiotics could cause accumulation of antibiotic resistance determinants in the gut microbiota of these animals.https://microbiologyjournal.org/genotypic-and-phenotypic-characterization-of-mbl-genes-in-pseudomonas-aeruginosa-isolates-from-the-non-hospital-environment/pseudomonas aeruginosaabattoirmbl genesimp-1vim-1multidrug resistancenigeria |
| spellingShingle | Chika Ejikeugwu Charles Esimone Ifeanyichukwu Iroha Peter Eze Malachy Ugwu Michael Adikwu Genotypic and Phenotypic Characterization of MBL Genes in Pseudomonas aeruginosa Isolates from the Non-hospital Environment Journal of Pure and Applied Microbiology pseudomonas aeruginosa abattoir mbl genes imp-1 vim-1 multidrug resistance nigeria |
| title | Genotypic and Phenotypic Characterization of MBL Genes in Pseudomonas aeruginosa Isolates from the Non-hospital Environment |
| title_full | Genotypic and Phenotypic Characterization of MBL Genes in Pseudomonas aeruginosa Isolates from the Non-hospital Environment |
| title_fullStr | Genotypic and Phenotypic Characterization of MBL Genes in Pseudomonas aeruginosa Isolates from the Non-hospital Environment |
| title_full_unstemmed | Genotypic and Phenotypic Characterization of MBL Genes in Pseudomonas aeruginosa Isolates from the Non-hospital Environment |
| title_short | Genotypic and Phenotypic Characterization of MBL Genes in Pseudomonas aeruginosa Isolates from the Non-hospital Environment |
| title_sort | genotypic and phenotypic characterization of mbl genes in pseudomonas aeruginosa isolates from the non hospital environment |
| topic | pseudomonas aeruginosa abattoir mbl genes imp-1 vim-1 multidrug resistance nigeria |
| url | https://microbiologyjournal.org/genotypic-and-phenotypic-characterization-of-mbl-genes-in-pseudomonas-aeruginosa-isolates-from-the-non-hospital-environment/ |
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