Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis
The use of DNA sequences analysis has been an important mean to distinguish and to identify populations of organisms at different levels. By molecular markers several complex organisms have been successful detected in plants for distinct aims. Ribosomal DNA (rDNA) has been used to evaluate genetic v...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2015-01-01
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| Series: | Bioscience Journal |
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| Online Access: | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/23126 |
| _version_ | 1818655249285513216 |
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| author | Enia Mara de Carvalho Antônia dos Reis Figueira José da Cruz Machado Dejânia Vieira de Araújo Cibele Ferreira Machado |
| author_facet | Enia Mara de Carvalho Antônia dos Reis Figueira José da Cruz Machado Dejânia Vieira de Araújo Cibele Ferreira Machado |
| author_sort | Enia Mara de Carvalho |
| collection | DOAJ |
| description | The use of DNA sequences analysis has been an important mean to distinguish and to identify populations of organisms at different levels. By molecular markers several complex organisms have been successful detected in plants for distinct aims. Ribosomal DNA (rDNA) has been used to evaluate genetic variability, microorganism phylogeny and to develop specific primers for detection of plant pathogens in plant tissues. In this study, the objective was to characterize isolates of Colletotrichum gossypii var. cephalosporioides and Colletotrichum gossypii, collected in different regions of Brazil, by analyzing the nucleotide sequence of rDNA regions. ITS1, ITS2, and the intervening 5.8S gene were amplified by PCR and their sequences compared to each other and to those from other species registered in the GenBank. The rDNA of isolates associated with Gossypium spp. showed sequence identities ranging from 96 to 100% in the ITS1 region, 98 to 100% in the 5.8S gene, and 97 to 100% in the ITS2 region. The sequences were submitted to UPGMA analysis, and according to the phylogenetic trees, the C. gossypii var. cephalosporioides and C. gossypii species clustered together along with isolates of Glomerella cingulata from mango and papaya, and thus no distinction could be made between isolates of those organisms. |
| first_indexed | 2024-12-17T03:06:41Z |
| format | Article |
| id | doaj.art-a21f4bd368a94cfda15ca19247175779 |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-17T03:06:41Z |
| publishDate | 2015-01-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-a21f4bd368a94cfda15ca192471757792022-12-21T22:05:55ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632015-01-0131310.14393/BJ-v31n3a2015-2312623126Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysisEnia Mara de Carvalho0Antônia dos Reis Figueira1José da Cruz Machado2Dejânia Vieira de Araújo3Cibele Ferreira Machado4Universidade Federal de LavrasUniversidade Federal de LavrasUniversidade Federal de LavrasUniversidade do Estado de Mato GrossoDow AgroSciencesThe use of DNA sequences analysis has been an important mean to distinguish and to identify populations of organisms at different levels. By molecular markers several complex organisms have been successful detected in plants for distinct aims. Ribosomal DNA (rDNA) has been used to evaluate genetic variability, microorganism phylogeny and to develop specific primers for detection of plant pathogens in plant tissues. In this study, the objective was to characterize isolates of Colletotrichum gossypii var. cephalosporioides and Colletotrichum gossypii, collected in different regions of Brazil, by analyzing the nucleotide sequence of rDNA regions. ITS1, ITS2, and the intervening 5.8S gene were amplified by PCR and their sequences compared to each other and to those from other species registered in the GenBank. The rDNA of isolates associated with Gossypium spp. showed sequence identities ranging from 96 to 100% in the ITS1 region, 98 to 100% in the 5.8S gene, and 97 to 100% in the ITS2 region. The sequences were submitted to UPGMA analysis, and according to the phylogenetic trees, the C. gossypii var. cephalosporioides and C. gossypii species clustered together along with isolates of Glomerella cingulata from mango and papaya, and thus no distinction could be made between isolates of those organisms.http://www.seer.ufu.br/index.php/biosciencejournal/article/view/23126colletotrichum gossypiicolletotrichum gossypii var. cephalosporioidesrdnaphylogenygossypium spp |
| spellingShingle | Enia Mara de Carvalho Antônia dos Reis Figueira José da Cruz Machado Dejânia Vieira de Araújo Cibele Ferreira Machado Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis Bioscience Journal colletotrichum gossypii colletotrichum gossypii var. cephalosporioides rdna phylogeny gossypium spp |
| title | Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis |
| title_full | Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis |
| title_fullStr | Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis |
| title_full_unstemmed | Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis |
| title_short | Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis |
| title_sort | variability of seed borne colletotrichum strains in cotton based on its1 and its2 ribossomal genes analysis |
| topic | colletotrichum gossypii colletotrichum gossypii var. cephalosporioides rdna phylogeny gossypium spp |
| url | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/23126 |
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