Photoperiodic Changes in Both Hypothalamus Neurotransmitters and Circulating Gonadal Steroids Metabolomic Profiles in Relation to Seasonal Reproduction in Male Quail
Both hypothalamic neurotransmitters and serum steroid hormones are impacted by photoperiod and have effects on physiology and seasonal reproductive. However, the relationship between circulating gonadal steroids and hypothalamic neurotransmitters underlying different photoperiod is still unclear. To...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.824228/full |
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| author | Yanglong Xu Yanglong Xu Danli Jiang Danli Jiang Jiaxin Liu Jiaxin Liu Yuting Fu Yuting Fu Yan Song Yan Song Di Fan Di Fan Xuefei Huang Xuefei Huang Sui Liufu Sui Liufu Jianqiu Pan Jianqiu Pan Hongjia Ouyang Hongjia Ouyang Yunbo Tian Yunbo Tian Xu Shen Xu Shen Yunmao Huang Yunmao Huang |
| author_facet | Yanglong Xu Yanglong Xu Danli Jiang Danli Jiang Jiaxin Liu Jiaxin Liu Yuting Fu Yuting Fu Yan Song Yan Song Di Fan Di Fan Xuefei Huang Xuefei Huang Sui Liufu Sui Liufu Jianqiu Pan Jianqiu Pan Hongjia Ouyang Hongjia Ouyang Yunbo Tian Yunbo Tian Xu Shen Xu Shen Yunmao Huang Yunmao Huang |
| author_sort | Yanglong Xu |
| collection | DOAJ |
| description | Both hypothalamic neurotransmitters and serum steroid hormones are impacted by photoperiod and have effects on physiology and seasonal reproductive. However, the relationship between circulating gonadal steroids and hypothalamic neurotransmitters underlying different photoperiod is still unclear. To further understand the crosstalk of neurotransmitters and steroids in seasonal reproduction, metabolic changes of 27 neurotransmitters concentrated in hypothalamus tissues and 42 steroids hormones in serum were assessed during two artificial photoperiodic programs. The results showed that photoperiod induce testicular atrophy and recrudescence. In L-to-S groups, significantly decreased levels of testosterone concentration were found in serum (P < 0.001) and increased 11-Dehydrocorticosterone (P < 0.05); Testosterone were almost undetectable at SD_14d. In addition, the hypothalamus exhibited significantly increased arginine and 4-aminobutyric acid (GABA) concentration and decreased serotonin and epinephrine content (P < 0.01 or P < 0.05). Accordingly, serum testosterone and androstenedione became detectable at LD_3d in the S-to-L group and were markedly increase at LD_7d. Furthermore, Serum androstenedione showed a significant increase with long light expose (P < 0.01). Additionally, the hypothalamus exhibited both significantly increased L.Tryptophan and phenylalanine concentration, as well as decreased L-glutamine and L-glutamine.acid content (P < 0.01 or P < 0.05). Serotonin metabolism showed significant differences between L-to-S group and S-to-L group. Furthermore, in the correlation analysis, serum testosterone had a positive correlation with 5-Hydroxyindole-3-acetic acid (5-HIAA), while Androstenedione was significantly negative with L.Tryptophan in L-to-S (P < 0.05). However, in S-to-L group, serum testosterone showed strong negative correlation with both serotonin and 5-HIAA (P < 0.05), but positive correlation with L.Tryptophan (P < 0.01), while Androstenedione was significantly negative correlation with both serotonin (P < 0.05) and L-Glutamine (P < 0.01). Photoperiod also had significant effects on the mRNA expression. We found significant differences in gene expression patterns of both serotonin signaling and steroid biosynthesis, while MAOB, NR5A1, and 3β-HSD showed an opposite tendency between two groups. Taken together, our results revealed that circulating gonadal steroids and hypothalamic neurotransmitters were significantly impact quail’s seasonal reproduction. Circulating gonadal steroids have different effects on neurotransmitter at different photoperiodism, which may coordinately influence the seasonal reproduction of quails. |
| first_indexed | 2024-04-13T15:35:34Z |
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| last_indexed | 2024-04-13T15:35:34Z |
| publishDate | 2022-03-01 |
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| series | Frontiers in Physiology |
| spelling | doaj.art-a220c116a0664d3bb06a5212ee3828eb2022-12-22T02:41:16ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-03-011310.3389/fphys.2022.824228824228Photoperiodic Changes in Both Hypothalamus Neurotransmitters and Circulating Gonadal Steroids Metabolomic Profiles in Relation to Seasonal Reproduction in Male QuailYanglong Xu0Yanglong Xu1Danli Jiang2Danli Jiang3Jiaxin Liu4Jiaxin Liu5Yuting Fu6Yuting Fu7Yan Song8Yan Song9Di Fan10Di Fan11Xuefei Huang12Xuefei Huang13Sui Liufu14Sui Liufu15Jianqiu Pan16Jianqiu Pan17Hongjia Ouyang18Hongjia Ouyang19Yunbo Tian20Yunbo Tian21Xu Shen22Xu Shen23Yunmao Huang24Yunmao Huang25College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, ChinaBoth hypothalamic neurotransmitters and serum steroid hormones are impacted by photoperiod and have effects on physiology and seasonal reproductive. However, the relationship between circulating gonadal steroids and hypothalamic neurotransmitters underlying different photoperiod is still unclear. To further understand the crosstalk of neurotransmitters and steroids in seasonal reproduction, metabolic changes of 27 neurotransmitters concentrated in hypothalamus tissues and 42 steroids hormones in serum were assessed during two artificial photoperiodic programs. The results showed that photoperiod induce testicular atrophy and recrudescence. In L-to-S groups, significantly decreased levels of testosterone concentration were found in serum (P < 0.001) and increased 11-Dehydrocorticosterone (P < 0.05); Testosterone were almost undetectable at SD_14d. In addition, the hypothalamus exhibited significantly increased arginine and 4-aminobutyric acid (GABA) concentration and decreased serotonin and epinephrine content (P < 0.01 or P < 0.05). Accordingly, serum testosterone and androstenedione became detectable at LD_3d in the S-to-L group and were markedly increase at LD_7d. Furthermore, Serum androstenedione showed a significant increase with long light expose (P < 0.01). Additionally, the hypothalamus exhibited both significantly increased L.Tryptophan and phenylalanine concentration, as well as decreased L-glutamine and L-glutamine.acid content (P < 0.01 or P < 0.05). Serotonin metabolism showed significant differences between L-to-S group and S-to-L group. Furthermore, in the correlation analysis, serum testosterone had a positive correlation with 5-Hydroxyindole-3-acetic acid (5-HIAA), while Androstenedione was significantly negative with L.Tryptophan in L-to-S (P < 0.05). However, in S-to-L group, serum testosterone showed strong negative correlation with both serotonin and 5-HIAA (P < 0.05), but positive correlation with L.Tryptophan (P < 0.01), while Androstenedione was significantly negative correlation with both serotonin (P < 0.05) and L-Glutamine (P < 0.01). Photoperiod also had significant effects on the mRNA expression. We found significant differences in gene expression patterns of both serotonin signaling and steroid biosynthesis, while MAOB, NR5A1, and 3β-HSD showed an opposite tendency between two groups. Taken together, our results revealed that circulating gonadal steroids and hypothalamic neurotransmitters were significantly impact quail’s seasonal reproduction. Circulating gonadal steroids have different effects on neurotransmitter at different photoperiodism, which may coordinately influence the seasonal reproduction of quails.https://www.frontiersin.org/articles/10.3389/fphys.2022.824228/fullphotoperiodneurotransmitterssteroid biosynthesisgene expressionquail |
| spellingShingle | Yanglong Xu Yanglong Xu Danli Jiang Danli Jiang Jiaxin Liu Jiaxin Liu Yuting Fu Yuting Fu Yan Song Yan Song Di Fan Di Fan Xuefei Huang Xuefei Huang Sui Liufu Sui Liufu Jianqiu Pan Jianqiu Pan Hongjia Ouyang Hongjia Ouyang Yunbo Tian Yunbo Tian Xu Shen Xu Shen Yunmao Huang Yunmao Huang Photoperiodic Changes in Both Hypothalamus Neurotransmitters and Circulating Gonadal Steroids Metabolomic Profiles in Relation to Seasonal Reproduction in Male Quail Frontiers in Physiology photoperiod neurotransmitters steroid biosynthesis gene expression quail |
| title | Photoperiodic Changes in Both Hypothalamus Neurotransmitters and Circulating Gonadal Steroids Metabolomic Profiles in Relation to Seasonal Reproduction in Male Quail |
| title_full | Photoperiodic Changes in Both Hypothalamus Neurotransmitters and Circulating Gonadal Steroids Metabolomic Profiles in Relation to Seasonal Reproduction in Male Quail |
| title_fullStr | Photoperiodic Changes in Both Hypothalamus Neurotransmitters and Circulating Gonadal Steroids Metabolomic Profiles in Relation to Seasonal Reproduction in Male Quail |
| title_full_unstemmed | Photoperiodic Changes in Both Hypothalamus Neurotransmitters and Circulating Gonadal Steroids Metabolomic Profiles in Relation to Seasonal Reproduction in Male Quail |
| title_short | Photoperiodic Changes in Both Hypothalamus Neurotransmitters and Circulating Gonadal Steroids Metabolomic Profiles in Relation to Seasonal Reproduction in Male Quail |
| title_sort | photoperiodic changes in both hypothalamus neurotransmitters and circulating gonadal steroids metabolomic profiles in relation to seasonal reproduction in male quail |
| topic | photoperiod neurotransmitters steroid biosynthesis gene expression quail |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.824228/full |
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