Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices
Aim Tissue engineering is a rapidly expanding field with regard to the use of biomaterials and stem cells in the orthopedic surgery. Many experimental studies have been done to understand the best characteristics of cells, materials and laboratory methods for safe clinical applications. The aim of t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Ariesdue
2015-03-01
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| Series: | Journal of Osseointegration |
| Subjects: | |
| Online Access: | http://www.journalofosseointegration.eu/wp-content/uploads/2015/03/JOsseointegr.2015_1_1.pdf |
| _version_ | 1818159302904381440 |
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| author | A. Pozzuoli C. Gardin R. Aldegheri E. Bressan M. Isola J. L. Calvo-Guirado C. Biz P. Arrigoni L. Feroni B. Zavan |
| author_facet | A. Pozzuoli C. Gardin R. Aldegheri E. Bressan M. Isola J. L. Calvo-Guirado C. Biz P. Arrigoni L. Feroni B. Zavan |
| author_sort | A. Pozzuoli |
| collection | DOAJ |
| description | Aim Tissue engineering is a rapidly expanding field with regard to the use of biomaterials and stem cells in the orthopedic surgery. Many experimental studies have been done to understand the best characteristics of cells, materials and laboratory methods for safe clinical applications. The aim of this study was to compare the ability of 2 different human demineralized bone matrices (DBMs), the one enriched and the other not enriched with hyaluronic acid, to stimulate in vitro the proliferation and the osteogenic differentiation of human adipose-derived stem cells (ADSCs) seeded onto an osteoconductive scaffold.
Materials and Methods ADSCs were isolated, by enzymatic digestion, from abdominal adipose tissue of 5 patients undergoing cosmetic lipoaspiration surgery. ADSCs were then seeded onto a 3D scaffold in the presence of the two different osteoinductive matrices of human demineralized bone and evaluated for proliferation and osteogenic differentiation. The safety of the methods was verified using array-Comparative Genomic Hybridization (array-CGH).
Results ADSCs were able to differentiate in osteogenic sense. Both DBMs showed the ability to induce osteogenic differentiation of the cells.
Conclusion array-CGH showed no changes at genome level, thus confirming the safety of materials and methods. |
| first_indexed | 2024-12-11T15:43:50Z |
| format | Article |
| id | doaj.art-a2232f291e6142d18b7835a0ceeee044 |
| institution | Directory Open Access Journal |
| issn | 2036-413X 2036-4121 |
| language | English |
| last_indexed | 2024-12-11T15:43:50Z |
| publishDate | 2015-03-01 |
| publisher | Ariesdue |
| record_format | Article |
| series | Journal of Osseointegration |
| spelling | doaj.art-a2232f291e6142d18b7835a0ceeee0442022-12-22T00:59:45ZengAriesdueJournal of Osseointegration2036-413X2036-41212015-03-017127Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matricesA. Pozzuoli0C. Gardin1R. Aldegheri2E. Bressan3M. Isola4J. L. Calvo-Guirado5C. Biz6P. Arrigoni7L. Feroni8B. Zavan9Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Padua, Italy Department of Biomedical Sciences, University of Padua, Padua, ItalyDepartment of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Padua, ItalyDepartment of Neurosciences, University of Padua, Padua, ItalyDepartment of Animal Medicine, Production and Health (MAPS)Department of General Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Padua, Italy Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Padua, Italy Department of Biomedical Sciences, University of Padua, Padua, Italy Department of Biomedical Sciences, University of Padua, Padua, ItalyAim Tissue engineering is a rapidly expanding field with regard to the use of biomaterials and stem cells in the orthopedic surgery. Many experimental studies have been done to understand the best characteristics of cells, materials and laboratory methods for safe clinical applications. The aim of this study was to compare the ability of 2 different human demineralized bone matrices (DBMs), the one enriched and the other not enriched with hyaluronic acid, to stimulate in vitro the proliferation and the osteogenic differentiation of human adipose-derived stem cells (ADSCs) seeded onto an osteoconductive scaffold. Materials and Methods ADSCs were isolated, by enzymatic digestion, from abdominal adipose tissue of 5 patients undergoing cosmetic lipoaspiration surgery. ADSCs were then seeded onto a 3D scaffold in the presence of the two different osteoinductive matrices of human demineralized bone and evaluated for proliferation and osteogenic differentiation. The safety of the methods was verified using array-Comparative Genomic Hybridization (array-CGH). Results ADSCs were able to differentiate in osteogenic sense. Both DBMs showed the ability to induce osteogenic differentiation of the cells. Conclusion array-CGH showed no changes at genome level, thus confirming the safety of materials and methods.http://www.journalofosseointegration.eu/wp-content/uploads/2015/03/JOsseointegr.2015_1_1.pdfAdipose-derived stem cellsArray-CGHDemineralized bone matricesOsteogenesis |
| spellingShingle | A. Pozzuoli C. Gardin R. Aldegheri E. Bressan M. Isola J. L. Calvo-Guirado C. Biz P. Arrigoni L. Feroni B. Zavan Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices Journal of Osseointegration Adipose-derived stem cells Array-CGH Demineralized bone matrices Osteogenesis |
| title | Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices |
| title_full | Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices |
| title_fullStr | Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices |
| title_full_unstemmed | Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices |
| title_short | Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices |
| title_sort | genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices |
| topic | Adipose-derived stem cells Array-CGH Demineralized bone matrices Osteogenesis |
| url | http://www.journalofosseointegration.eu/wp-content/uploads/2015/03/JOsseointegr.2015_1_1.pdf |
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