Metagenome and Resistome Analysis of Beta-Lactam-Resistant Bacteria Isolated from River Waters in Surabaya, Indonesia
Antimicrobial agents are administered to humans and livestock, and bacterial antimicrobial resistance (AMR) and antimicrobial agents are released into the environment. In this study, to investigate the trend of AMR in humans, livestock, and the environment, we performed a metagenomic analysis of mul...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/12/1/199 |
| _version_ | 1797339563340857344 |
|---|---|
| author | Ryohei Nomoto Kayo Osawa Shohiro Kinoshita Koichi Kitagawa Noriko Nakanishi Rosantia Sarassari Dadik Raharjo Masato Fujisawa Kuntaman Kuntaman Toshiro Shirakawa |
| author_facet | Ryohei Nomoto Kayo Osawa Shohiro Kinoshita Koichi Kitagawa Noriko Nakanishi Rosantia Sarassari Dadik Raharjo Masato Fujisawa Kuntaman Kuntaman Toshiro Shirakawa |
| author_sort | Ryohei Nomoto |
| collection | DOAJ |
| description | Antimicrobial agents are administered to humans and livestock, and bacterial antimicrobial resistance (AMR) and antimicrobial agents are released into the environment. In this study, to investigate the trend of AMR in humans, livestock, and the environment, we performed a metagenomic analysis of multidrug-resistant bacteria with CHROMagar ESBL in environmental river water samples, which were collected using syringe filter units from waters near hospitals, downtown areas, residential areas, and water treatment plants in Surabaya, Indonesia. Our results showed that <i>Acinetobacter</i>, <i>Pseudomonas</i>, <i>Aeromonas</i>, <i>Enterobacter</i>, <i>Escherichia</i>, and <i>Klebsiella</i> grew in CHROMagar ESBL; they were most frequently detected in water samples from rivers surrounding hospitals contaminated with various AMR genes (ARGs) in high levels. These results identified bacteria as ARG reservoirs and revealed that hospitals could be sources for various ARGs disseminated into the environment. In conclusion, this study details a novel metagenomic analysis of collected bacteria in environmental water samples using a syringe filter unit for an AMR epidemiological study based on the One Health approach. |
| first_indexed | 2024-03-08T09:50:00Z |
| format | Article |
| id | doaj.art-a2234064c2dc4804858a8ed03e4278f6 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T09:50:00Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a2234064c2dc4804858a8ed03e4278f62024-01-29T14:06:57ZengMDPI AGMicroorganisms2076-26072024-01-0112119910.3390/microorganisms12010199Metagenome and Resistome Analysis of Beta-Lactam-Resistant Bacteria Isolated from River Waters in Surabaya, IndonesiaRyohei Nomoto0Kayo Osawa1Shohiro Kinoshita2Koichi Kitagawa3Noriko Nakanishi4Rosantia Sarassari5Dadik Raharjo6Masato Fujisawa7Kuntaman Kuntaman8Toshiro Shirakawa9Department of Infectious Diseases, Kobe Institute of Health, Kobe 650-0046, JapanDepartment of Medical Technology, Kobe Tokiwa University, Kobe 653-0838, JapanDivision of Advanced Medical Science, Kobe University Graduate School of Science, Technology and Innovation, Kobe 650-0017, JapanDivision of Advanced Medical Science, Kobe University Graduate School of Science, Technology and Innovation, Kobe 650-0017, JapanDepartment of Infectious Diseases, Kobe Institute of Health, Kobe 650-0046, JapanDepartment of Microbiology, Faculty of Medicine, Airlangga University, Surabaya 60132, IndonesiaInstitute of Tropical Disease, Airlangga University, Surabaya 60286, IndonesiaDivision of Urology, Department of Organ Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Microbiology, Faculty of Medicine, Airlangga University, Surabaya 60132, IndonesiaDivision of Advanced Medical Science, Kobe University Graduate School of Science, Technology and Innovation, Kobe 650-0017, JapanAntimicrobial agents are administered to humans and livestock, and bacterial antimicrobial resistance (AMR) and antimicrobial agents are released into the environment. In this study, to investigate the trend of AMR in humans, livestock, and the environment, we performed a metagenomic analysis of multidrug-resistant bacteria with CHROMagar ESBL in environmental river water samples, which were collected using syringe filter units from waters near hospitals, downtown areas, residential areas, and water treatment plants in Surabaya, Indonesia. Our results showed that <i>Acinetobacter</i>, <i>Pseudomonas</i>, <i>Aeromonas</i>, <i>Enterobacter</i>, <i>Escherichia</i>, and <i>Klebsiella</i> grew in CHROMagar ESBL; they were most frequently detected in water samples from rivers surrounding hospitals contaminated with various AMR genes (ARGs) in high levels. These results identified bacteria as ARG reservoirs and revealed that hospitals could be sources for various ARGs disseminated into the environment. In conclusion, this study details a novel metagenomic analysis of collected bacteria in environmental water samples using a syringe filter unit for an AMR epidemiological study based on the One Health approach.https://www.mdpi.com/2076-2607/12/1/199antimicrobial-resistant bacteria (AMR)environmental riverwhole genome sequencing (WGS)antimicrobial resistance genes (ARGs)public health |
| spellingShingle | Ryohei Nomoto Kayo Osawa Shohiro Kinoshita Koichi Kitagawa Noriko Nakanishi Rosantia Sarassari Dadik Raharjo Masato Fujisawa Kuntaman Kuntaman Toshiro Shirakawa Metagenome and Resistome Analysis of Beta-Lactam-Resistant Bacteria Isolated from River Waters in Surabaya, Indonesia Microorganisms antimicrobial-resistant bacteria (AMR) environmental river whole genome sequencing (WGS) antimicrobial resistance genes (ARGs) public health |
| title | Metagenome and Resistome Analysis of Beta-Lactam-Resistant Bacteria Isolated from River Waters in Surabaya, Indonesia |
| title_full | Metagenome and Resistome Analysis of Beta-Lactam-Resistant Bacteria Isolated from River Waters in Surabaya, Indonesia |
| title_fullStr | Metagenome and Resistome Analysis of Beta-Lactam-Resistant Bacteria Isolated from River Waters in Surabaya, Indonesia |
| title_full_unstemmed | Metagenome and Resistome Analysis of Beta-Lactam-Resistant Bacteria Isolated from River Waters in Surabaya, Indonesia |
| title_short | Metagenome and Resistome Analysis of Beta-Lactam-Resistant Bacteria Isolated from River Waters in Surabaya, Indonesia |
| title_sort | metagenome and resistome analysis of beta lactam resistant bacteria isolated from river waters in surabaya indonesia |
| topic | antimicrobial-resistant bacteria (AMR) environmental river whole genome sequencing (WGS) antimicrobial resistance genes (ARGs) public health |
| url | https://www.mdpi.com/2076-2607/12/1/199 |
| work_keys_str_mv | AT ryoheinomoto metagenomeandresistomeanalysisofbetalactamresistantbacteriaisolatedfromriverwatersinsurabayaindonesia AT kayoosawa metagenomeandresistomeanalysisofbetalactamresistantbacteriaisolatedfromriverwatersinsurabayaindonesia AT shohirokinoshita metagenomeandresistomeanalysisofbetalactamresistantbacteriaisolatedfromriverwatersinsurabayaindonesia AT koichikitagawa metagenomeandresistomeanalysisofbetalactamresistantbacteriaisolatedfromriverwatersinsurabayaindonesia AT norikonakanishi metagenomeandresistomeanalysisofbetalactamresistantbacteriaisolatedfromriverwatersinsurabayaindonesia AT rosantiasarassari metagenomeandresistomeanalysisofbetalactamresistantbacteriaisolatedfromriverwatersinsurabayaindonesia AT dadikraharjo metagenomeandresistomeanalysisofbetalactamresistantbacteriaisolatedfromriverwatersinsurabayaindonesia AT masatofujisawa metagenomeandresistomeanalysisofbetalactamresistantbacteriaisolatedfromriverwatersinsurabayaindonesia AT kuntamankuntaman metagenomeandresistomeanalysisofbetalactamresistantbacteriaisolatedfromriverwatersinsurabayaindonesia AT toshiroshirakawa metagenomeandresistomeanalysisofbetalactamresistantbacteriaisolatedfromriverwatersinsurabayaindonesia |