Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF
The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimpla...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Dorota Pierzchała Kamila Liput Agnieszka Korwin-Kossakowska Magdalena Ogłuszka Ewa Poławska Agata Nawrocka Paweł Urbański Aleksandra Ciepłoch Edyta Juszczuk-Kubiak Adam Lepczyński Brygida Ślaska Krzysztof Kowal Marinus F. W. te Pas Magdalena Śmiech Paweł Leszczyński Hiroaki Taniguchi Leyland Fraser Przemysław Sobiech Mateusz Sachajko Magdalena Herudzinska Chandra S. Pareek Mariusz Pierzchała |
| author_facet | Dorota Pierzchała Kamila Liput Agnieszka Korwin-Kossakowska Magdalena Ogłuszka Ewa Poławska Agata Nawrocka Paweł Urbański Aleksandra Ciepłoch Edyta Juszczuk-Kubiak Adam Lepczyński Brygida Ślaska Krzysztof Kowal Marinus F. W. te Pas Magdalena Śmiech Paweł Leszczyński Hiroaki Taniguchi Leyland Fraser Przemysław Sobiech Mateusz Sachajko Magdalena Herudzinska Chandra S. Pareek Mariusz Pierzchała |
| author_sort | Dorota Pierzchała |
| collection | DOAJ |
| description | The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and ClueGO<sup>TM</sup> approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts. |
| first_indexed | 2024-03-10T10:08:42Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:08:42Z |
| publishDate | 2021-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a2254f9a8989483eb27c6430cdac034f2023-11-22T01:21:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012213672010.3390/ijms22136720Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOFDorota Pierzchała0Kamila Liput1Agnieszka Korwin-Kossakowska2Magdalena Ogłuszka3Ewa Poławska4Agata Nawrocka5Paweł Urbański6Aleksandra Ciepłoch7Edyta Juszczuk-Kubiak8Adam Lepczyński9Brygida Ślaska10Krzysztof Kowal11Marinus F. W. te Pas12Magdalena Śmiech13Paweł Leszczyński14Hiroaki Taniguchi15Leyland Fraser16Przemysław Sobiech17Mateusz Sachajko18Magdalena Herudzinska19Chandra S. Pareek20Mariusz Pierzchała21Department of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandDepartment of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandDepartment of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandDepartment of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandDepartment of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandDepartment of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandDepartment of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandDepartment of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandLaboratory of Biotechnology and Molecular Engineering, Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, State Research Institute, Rakowiecka 36 Street, 02-532 Warsaw, PolandDepartment of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology, ul. K. Janickiego 32, 71-270 Szczecin, PolandFaculty of Animal Sciences and Bioeconomy, Institute of Biological Bases of Animal Production, University of Life Sciences in Lublin, ul. Akademicka 13, 20-950 Lublin, PolandFaculty of Animal Sciences and Bioeconomy, Institute of Biological Bases of Animal Production, University of Life Sciences in Lublin, ul. Akademicka 13, 20-950 Lublin, PolandThe Netherlands Wageningen UR Livestock Research, Animal Breeding and Genomics, P.O. Box 9101, 6700 HB Wageningen, The NetherlandsDepartment of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandDepartment of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandDepartment of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandDepartment of Animal Biochemistry and Biotechnology, Faculty of Animal Bio-Engineering, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 5, 10-719 Olsztyn, PolandInternal Disease Unit, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandFaculty of Biological and Veterinary Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University, ul. Gagarina 7, 87-100 Toruń, PolandFaculty of Biological and Veterinary Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University, ul. Gagarina 7, 87-100 Toruń, PolandFaculty of Biological and Veterinary Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University, ul. Gagarina 7, 87-100 Toruń, PolandDepartment of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, PolandThe molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and ClueGO<sup>TM</sup> approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts.https://www.mdpi.com/1422-0067/22/13/6720differentially expressed proteinspregnancyendometriumimplantationproteomePolish Large White |
| spellingShingle | Dorota Pierzchała Kamila Liput Agnieszka Korwin-Kossakowska Magdalena Ogłuszka Ewa Poławska Agata Nawrocka Paweł Urbański Aleksandra Ciepłoch Edyta Juszczuk-Kubiak Adam Lepczyński Brygida Ślaska Krzysztof Kowal Marinus F. W. te Pas Magdalena Śmiech Paweł Leszczyński Hiroaki Taniguchi Leyland Fraser Przemysław Sobiech Mateusz Sachajko Magdalena Herudzinska Chandra S. Pareek Mariusz Pierzchała Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF International Journal of Molecular Sciences differentially expressed proteins pregnancy endometrium implantation proteome Polish Large White |
| title | Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF |
| title_full | Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF |
| title_fullStr | Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF |
| title_full_unstemmed | Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF |
| title_short | Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF |
| title_sort | molecular characterisation of uterine endometrial proteins during early stages of pregnancy in pigs by maldi tof tof |
| topic | differentially expressed proteins pregnancy endometrium implantation proteome Polish Large White |
| url | https://www.mdpi.com/1422-0067/22/13/6720 |
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