The Potential of Novel Synthesized Carbon Dots Derived Resveratrol Using One-Pot Green Method in Accelerating in vivo Wound Healing
Huaiyi Cheng,* Yifan Zhao,* Yue Wang,* Yuxi Hou, Ran Zhang, Mingrui Zong, Lingxiang Sun, Yingyu Liu, Jin Qi, Xiuping Wu, Bing Li School and Hospital of Stomatology, Shanxi Medical University, Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2023-11-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/the-potential-of-novel-synthesized-carbon-dots-derived-resveratrol-usi-peer-reviewed-fulltext-article-IJN |
| _version_ | 1797572345711296512 |
|---|---|
| author | Cheng H Zhao Y Wang Y Hou Y Zhang R Zong M Sun L Liu Y Qi J Wu X Li B |
| author_facet | Cheng H Zhao Y Wang Y Hou Y Zhang R Zong M Sun L Liu Y Qi J Wu X Li B |
| author_sort | Cheng H |
| collection | DOAJ |
| description | Huaiyi Cheng,* Yifan Zhao,* Yue Wang,* Yuxi Hou, Ran Zhang, Mingrui Zong, Lingxiang Sun, Yingyu Liu, Jin Qi, Xiuping Wu, Bing Li School and Hospital of Stomatology, Shanxi Medical University, Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University, Taiyuan, 030001, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bing Li; Xiuping Wu, Email libing1975vip@163.com; 77wxp@163.comBackground: Carbon dots (CDs), a novel nanomaterial, have gained significant attention over the past decade due to their remarkable fluorescence properties, low toxicity, and biocompatibility. These characteristics make them promising in various applications, especially in biomedicine. However, most CDs are currently synthesized using chemical materials, and their biocompatibility falls short of natural compounds. Research on extracting CDs from natural sources is limited, and their potential in biomedicine remains largely unexplored.Methods: We extracted CDs from resveratrol, a natural plant compound, and enhanced their water solubility using citric acid. Characterization of resveratrol-based carbon dots (RES-CDs) was carried out using various techniques, including UV-Vis, SEM, TEM, FTIR, XRD, and fluorescence spectroscopy. Extensive biocompatibility tests, wound healing assays, cell migration studies, and angiogenesis experiments were conducted using human umbilical vein endothelial cells (HUVEC). In addition, we investigated the biocompatibility and wound healing potential of RES-CDs in an in vivo rat model of inflammation.Results: RES-CDs exhibited stable yellow-green fluorescence under 365-nanometer ultraviolet light and demonstrated excellent biocompatibility. In wound healing experiments, RES-CDs outperformed resveratrol in terms of cell scratch healing, migration, and tube formation. In a rat skin defect model, RES-CDs promoted wound healing and stimulated the formation of blood vessels and tissue regeneration near the wound site, as evidenced by increased CD31 and VEGF expression.Conclusion: Resveratrol-derived CDs with enhanced water solubility show superior performance in tissue healing compared to resveratrol. This discovery opens new possibilities for the clinical application of resveratrol-based carbon dots. Keywords: carbon dots, wound healing, resveratrol, fluorescence, bio-imaging, biocompatibility |
| first_indexed | 2024-03-10T20:54:56Z |
| format | Article |
| id | doaj.art-a226eef1c9b846718288cdbb6bbd441f |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-03-10T20:54:56Z |
| publishDate | 2023-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-a226eef1c9b846718288cdbb6bbd441f2023-11-19T18:03:58ZengDove Medical PressInternational Journal of Nanomedicine1178-20132023-11-01Volume 186813682888291The Potential of Novel Synthesized Carbon Dots Derived Resveratrol Using One-Pot Green Method in Accelerating in vivo Wound HealingCheng HZhao YWang YHou YZhang RZong MSun LLiu YQi JWu XLi BHuaiyi Cheng,* Yifan Zhao,* Yue Wang,* Yuxi Hou, Ran Zhang, Mingrui Zong, Lingxiang Sun, Yingyu Liu, Jin Qi, Xiuping Wu, Bing Li School and Hospital of Stomatology, Shanxi Medical University, Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University, Taiyuan, 030001, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bing Li; Xiuping Wu, Email libing1975vip@163.com; 77wxp@163.comBackground: Carbon dots (CDs), a novel nanomaterial, have gained significant attention over the past decade due to their remarkable fluorescence properties, low toxicity, and biocompatibility. These characteristics make them promising in various applications, especially in biomedicine. However, most CDs are currently synthesized using chemical materials, and their biocompatibility falls short of natural compounds. Research on extracting CDs from natural sources is limited, and their potential in biomedicine remains largely unexplored.Methods: We extracted CDs from resveratrol, a natural plant compound, and enhanced their water solubility using citric acid. Characterization of resveratrol-based carbon dots (RES-CDs) was carried out using various techniques, including UV-Vis, SEM, TEM, FTIR, XRD, and fluorescence spectroscopy. Extensive biocompatibility tests, wound healing assays, cell migration studies, and angiogenesis experiments were conducted using human umbilical vein endothelial cells (HUVEC). In addition, we investigated the biocompatibility and wound healing potential of RES-CDs in an in vivo rat model of inflammation.Results: RES-CDs exhibited stable yellow-green fluorescence under 365-nanometer ultraviolet light and demonstrated excellent biocompatibility. In wound healing experiments, RES-CDs outperformed resveratrol in terms of cell scratch healing, migration, and tube formation. In a rat skin defect model, RES-CDs promoted wound healing and stimulated the formation of blood vessels and tissue regeneration near the wound site, as evidenced by increased CD31 and VEGF expression.Conclusion: Resveratrol-derived CDs with enhanced water solubility show superior performance in tissue healing compared to resveratrol. This discovery opens new possibilities for the clinical application of resveratrol-based carbon dots. Keywords: carbon dots, wound healing, resveratrol, fluorescence, bio-imaging, biocompatibilityhttps://www.dovepress.com/the-potential-of-novel-synthesized-carbon-dots-derived-resveratrol-usi-peer-reviewed-fulltext-article-IJNcarbon dotswound healingresveratrolfluorescencebio-imagingbiocompatibility. |
| spellingShingle | Cheng H Zhao Y Wang Y Hou Y Zhang R Zong M Sun L Liu Y Qi J Wu X Li B The Potential of Novel Synthesized Carbon Dots Derived Resveratrol Using One-Pot Green Method in Accelerating in vivo Wound Healing International Journal of Nanomedicine carbon dots wound healing resveratrol fluorescence bio-imaging biocompatibility. |
| title | The Potential of Novel Synthesized Carbon Dots Derived Resveratrol Using One-Pot Green Method in Accelerating in vivo Wound Healing |
| title_full | The Potential of Novel Synthesized Carbon Dots Derived Resveratrol Using One-Pot Green Method in Accelerating in vivo Wound Healing |
| title_fullStr | The Potential of Novel Synthesized Carbon Dots Derived Resveratrol Using One-Pot Green Method in Accelerating in vivo Wound Healing |
| title_full_unstemmed | The Potential of Novel Synthesized Carbon Dots Derived Resveratrol Using One-Pot Green Method in Accelerating in vivo Wound Healing |
| title_short | The Potential of Novel Synthesized Carbon Dots Derived Resveratrol Using One-Pot Green Method in Accelerating in vivo Wound Healing |
| title_sort | potential of novel synthesized carbon dots derived resveratrol using one pot green method in accelerating in vivo wound healing |
| topic | carbon dots wound healing resveratrol fluorescence bio-imaging biocompatibility. |
| url | https://www.dovepress.com/the-potential-of-novel-synthesized-carbon-dots-derived-resveratrol-usi-peer-reviewed-fulltext-article-IJN |
| work_keys_str_mv | AT chengh thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT zhaoy thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT wangy thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT houy thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT zhangr thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT zongm thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT sunl thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT liuy thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT qij thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT wux thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT lib thepotentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT chengh potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT zhaoy potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT wangy potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT houy potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT zhangr potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT zongm potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT sunl potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT liuy potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT qij potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT wux potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing AT lib potentialofnovelsynthesizedcarbondotsderivedresveratrolusingonepotgreenmethodinacceleratinginvivowoundhealing |