Evaluation of the Antibacterial Material Production in the Fermentation of <i>Bacillus</i> <i>amyloliquefaciens-9</i> from Whitespotted Bamboo Shark (<i>Chiloscyllium plagiosum</i>)
<i>Bacillus amyloliquefaciens-9</i> (GBacillus-9), which is isolated from the intestinal tract of the white-spotted bamboo shark (<i>Chiloscyllium plagiosum</i>), can secrete potential antibacterial materials, such as β-1,3-1,4-glucanase and some antimicrobial peptid...
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| Format: | Article |
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MDPI AG
2020-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/2/119 |
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| author | Wenjie Zhang Ling Wei Rong Xu Guodong Lin Huijie Xin Zhengbing Lv Hong Qian Hengbo Shi |
| author_facet | Wenjie Zhang Ling Wei Rong Xu Guodong Lin Huijie Xin Zhengbing Lv Hong Qian Hengbo Shi |
| author_sort | Wenjie Zhang |
| collection | DOAJ |
| description | <i>Bacillus amyloliquefaciens-9</i> (GBacillus-9), which is isolated from the intestinal tract of the white-spotted bamboo shark (<i>Chiloscyllium plagiosum</i>), can secrete potential antibacterial materials, such as β-1,3-1,4-glucanase and some antimicrobial peptides. However, the low fermentation production has hindered the development of GBacillus-9 as biological additives. In this study, the Plackett−Burman design and response surface methodology were used to optimize the fermentation conditions in a shake flask to obtain a higher yield and antibacterial activity of GBacillus-9. On the basis of the data from medium screening, M9 medium was selected as the basic medium for fermentation. The data from the single-factor experiment showed that sucrose had the highest antibacterial activity among the 10 carbon sources. The Plackett−Burman design identified sucrose, NH<sub>4</sub>Cl, and MgSO<sub>4</sub> as the major variables altering antibacterial activity. The optimal concentrations of these compounds to enhance antibacterial activity were assessed using the central composite design. Data showed that sucrose, NH<sub>4</sub>Cl, and MgSO<sub>4</sub> had the highest antibacterial activities at concentrations of 64.8, 1.84, and 0.08 g L<sup>−1</sup>, respectively. The data also showed that the optimal fermentation conditions for the antibacterial material production of GBacillus-9 were as follows: Inoculum volume of 5%, initial pH of 7.0, temperature of 36 °C, rotating speed of 180 rpm, and fermentation time of 10 h. The optimal fermentation medium and conditions achieved to improve the yield of antibacterial materials for GBacillus-9 can enhance the process of developing biological additives derived from GBacillus-9. |
| first_indexed | 2024-04-13T00:39:10Z |
| format | Article |
| id | doaj.art-a22d9773da80425abdef5d93837a4123 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T00:39:10Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a22d9773da80425abdef5d93837a41232022-12-22T03:10:15ZengMDPI AGMarine Drugs1660-33972020-02-0118211910.3390/md18020119md18020119Evaluation of the Antibacterial Material Production in the Fermentation of <i>Bacillus</i> <i>amyloliquefaciens-9</i> from Whitespotted Bamboo Shark (<i>Chiloscyllium plagiosum</i>)Wenjie Zhang0Ling Wei1Rong Xu2Guodong Lin3Huijie Xin4Zhengbing Lv5Hong Qian6Hengbo Shi7Zhejiang provincial key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang provincial key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang provincial key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang provincial key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang provincial key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang provincial key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang provincial key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang provincial key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China<i>Bacillus amyloliquefaciens-9</i> (GBacillus-9), which is isolated from the intestinal tract of the white-spotted bamboo shark (<i>Chiloscyllium plagiosum</i>), can secrete potential antibacterial materials, such as β-1,3-1,4-glucanase and some antimicrobial peptides. However, the low fermentation production has hindered the development of GBacillus-9 as biological additives. In this study, the Plackett−Burman design and response surface methodology were used to optimize the fermentation conditions in a shake flask to obtain a higher yield and antibacterial activity of GBacillus-9. On the basis of the data from medium screening, M9 medium was selected as the basic medium for fermentation. The data from the single-factor experiment showed that sucrose had the highest antibacterial activity among the 10 carbon sources. The Plackett−Burman design identified sucrose, NH<sub>4</sub>Cl, and MgSO<sub>4</sub> as the major variables altering antibacterial activity. The optimal concentrations of these compounds to enhance antibacterial activity were assessed using the central composite design. Data showed that sucrose, NH<sub>4</sub>Cl, and MgSO<sub>4</sub> had the highest antibacterial activities at concentrations of 64.8, 1.84, and 0.08 g L<sup>−1</sup>, respectively. The data also showed that the optimal fermentation conditions for the antibacterial material production of GBacillus-9 were as follows: Inoculum volume of 5%, initial pH of 7.0, temperature of 36 °C, rotating speed of 180 rpm, and fermentation time of 10 h. The optimal fermentation medium and conditions achieved to improve the yield of antibacterial materials for GBacillus-9 can enhance the process of developing biological additives derived from GBacillus-9.https://www.mdpi.com/1660-3397/18/2/119antibacterial materials<i>bacillus amyloliquefaciens</i>whitespotted bamboo sharkbiological additive |
| spellingShingle | Wenjie Zhang Ling Wei Rong Xu Guodong Lin Huijie Xin Zhengbing Lv Hong Qian Hengbo Shi Evaluation of the Antibacterial Material Production in the Fermentation of <i>Bacillus</i> <i>amyloliquefaciens-9</i> from Whitespotted Bamboo Shark (<i>Chiloscyllium plagiosum</i>) Marine Drugs antibacterial materials <i>bacillus amyloliquefaciens</i> whitespotted bamboo shark biological additive |
| title | Evaluation of the Antibacterial Material Production in the Fermentation of <i>Bacillus</i> <i>amyloliquefaciens-9</i> from Whitespotted Bamboo Shark (<i>Chiloscyllium plagiosum</i>) |
| title_full | Evaluation of the Antibacterial Material Production in the Fermentation of <i>Bacillus</i> <i>amyloliquefaciens-9</i> from Whitespotted Bamboo Shark (<i>Chiloscyllium plagiosum</i>) |
| title_fullStr | Evaluation of the Antibacterial Material Production in the Fermentation of <i>Bacillus</i> <i>amyloliquefaciens-9</i> from Whitespotted Bamboo Shark (<i>Chiloscyllium plagiosum</i>) |
| title_full_unstemmed | Evaluation of the Antibacterial Material Production in the Fermentation of <i>Bacillus</i> <i>amyloliquefaciens-9</i> from Whitespotted Bamboo Shark (<i>Chiloscyllium plagiosum</i>) |
| title_short | Evaluation of the Antibacterial Material Production in the Fermentation of <i>Bacillus</i> <i>amyloliquefaciens-9</i> from Whitespotted Bamboo Shark (<i>Chiloscyllium plagiosum</i>) |
| title_sort | evaluation of the antibacterial material production in the fermentation of i bacillus i i amyloliquefaciens 9 i from whitespotted bamboo shark i chiloscyllium plagiosum i |
| topic | antibacterial materials <i>bacillus amyloliquefaciens</i> whitespotted bamboo shark biological additive |
| url | https://www.mdpi.com/1660-3397/18/2/119 |
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