Potency Adjusted Blended Heparin of Bovine, Ovine, and Porcine Heparin Exhibit Comparable Biologic Effects to Referenced Single-Sourced Porcine Heparin
Introduction: Bovine and ovine mucosa represent alternate anticoagulants to porcine mucosa for production of unfractionated heparin (UFH). Standardized heparins from various sources can be blended and potency adjusted, blended heparins exhibit comparable effects as single-sourced porcine UFH. This s...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2023-03-01
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| Series: | Clinical and Applied Thrombosis/Hemostasis |
| Online Access: | https://doi.org/10.1177/10760296231163251 |
| _version_ | 1797872160268615680 |
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| author | Guy Olson BS Walter Jeske PhD Omer Iqbal MD Emily Krupa BS Amber Farooqui MD Fakiha Siddiqui BDS Debra Hoppensteadt PhD Ahmed Kouta PhD Jawed Fareed PhD |
| author_facet | Guy Olson BS Walter Jeske PhD Omer Iqbal MD Emily Krupa BS Amber Farooqui MD Fakiha Siddiqui BDS Debra Hoppensteadt PhD Ahmed Kouta PhD Jawed Fareed PhD |
| author_sort | Guy Olson BS |
| collection | DOAJ |
| description | Introduction: Bovine and ovine mucosa represent alternate anticoagulants to porcine mucosa for production of unfractionated heparin (UFH). Standardized heparins from various sources can be blended and potency adjusted, blended heparins exhibit comparable effects as single-sourced porcine UFH. This study evaluated the pharmacologic profile of blended heparin and compared their activities to that of single sourced porcine, ovine, and bovine heparins. Methods: The anticoagulant effects of gravimetric and potency-adjusted heparins were evaluated with aPTT, TT, anti-Xa, anti-IIa, ACT, and TGA studies. Protamine sulfate studies were used for neutralization potential of each of the individual heparins. Results: The potency-adjusted heparins demonstrated comparable aPTT, TT, anti-Xa, anti-IIa, and ACT values at all concentrations (U/mL). However, in gravimetric studies, bovine heparin consistently showed lower values with the exception of thrombin generation inhibition studies. The protamine sulfate neutralization studies demonstrated complete neutralization at all concentrations for the potency-adjusted heparins. However, at gravimetric concentrations, minor differences were noted in the neutralization profile in each of these heparins. Conclusion: These studies support the hypothesis that blended heparin from bovine, ovine, and porcine tissue, when standardized in unit-equivalent proportions, exhibits a comparable anticoagulant profile to the single species derived heparins. |
| first_indexed | 2024-04-10T00:55:23Z |
| format | Article |
| id | doaj.art-a22e14aa5cf148389f9dbf9a742d529d |
| institution | Directory Open Access Journal |
| issn | 1938-2723 |
| language | English |
| last_indexed | 2024-04-10T00:55:23Z |
| publishDate | 2023-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Clinical and Applied Thrombosis/Hemostasis |
| spelling | doaj.art-a22e14aa5cf148389f9dbf9a742d529d2023-03-13T10:33:33ZengSAGE PublishingClinical and Applied Thrombosis/Hemostasis1938-27232023-03-012910.1177/10760296231163251Potency Adjusted Blended Heparin of Bovine, Ovine, and Porcine Heparin Exhibit Comparable Biologic Effects to Referenced Single-Sourced Porcine HeparinGuy Olson BS0Walter Jeske PhD1Omer Iqbal MD2 Emily Krupa BS3Amber Farooqui MD4Fakiha Siddiqui BDS5Debra Hoppensteadt PhD6Ahmed Kouta PhD7Jawed Fareed PhD8 Cardiovascular Research Institute, Section of Hemostasis and Thrombosis, , Maywood, IL, USA Cardiovascular Research Institute, Section of Hemostasis and Thrombosis, , Maywood, IL, USA Cardiovascular Research Institute, Section of Hemostasis and Thrombosis, , Maywood, IL, USA Cardiovascular Research Institute, Section of Hemostasis and Thrombosis, , Maywood, IL, USA Cardiovascular Research Institute, Section of Hemostasis and Thrombosis, , Maywood, IL, USA Program in Health Sciences, , Murcia, Spain Cardiovascular Research Institute, Section of Hemostasis and Thrombosis, , Maywood, IL, USA Cardiovascular Research Institute, Section of Hemostasis and Thrombosis, , Maywood, IL, USA Cardiovascular Research Institute, Section of Hemostasis and Thrombosis, , Maywood, IL, USAIntroduction: Bovine and ovine mucosa represent alternate anticoagulants to porcine mucosa for production of unfractionated heparin (UFH). Standardized heparins from various sources can be blended and potency adjusted, blended heparins exhibit comparable effects as single-sourced porcine UFH. This study evaluated the pharmacologic profile of blended heparin and compared their activities to that of single sourced porcine, ovine, and bovine heparins. Methods: The anticoagulant effects of gravimetric and potency-adjusted heparins were evaluated with aPTT, TT, anti-Xa, anti-IIa, ACT, and TGA studies. Protamine sulfate studies were used for neutralization potential of each of the individual heparins. Results: The potency-adjusted heparins demonstrated comparable aPTT, TT, anti-Xa, anti-IIa, and ACT values at all concentrations (U/mL). However, in gravimetric studies, bovine heparin consistently showed lower values with the exception of thrombin generation inhibition studies. The protamine sulfate neutralization studies demonstrated complete neutralization at all concentrations for the potency-adjusted heparins. However, at gravimetric concentrations, minor differences were noted in the neutralization profile in each of these heparins. Conclusion: These studies support the hypothesis that blended heparin from bovine, ovine, and porcine tissue, when standardized in unit-equivalent proportions, exhibits a comparable anticoagulant profile to the single species derived heparins.https://doi.org/10.1177/10760296231163251 |
| spellingShingle | Guy Olson BS Walter Jeske PhD Omer Iqbal MD Emily Krupa BS Amber Farooqui MD Fakiha Siddiqui BDS Debra Hoppensteadt PhD Ahmed Kouta PhD Jawed Fareed PhD Potency Adjusted Blended Heparin of Bovine, Ovine, and Porcine Heparin Exhibit Comparable Biologic Effects to Referenced Single-Sourced Porcine Heparin Clinical and Applied Thrombosis/Hemostasis |
| title | Potency Adjusted Blended Heparin of Bovine, Ovine, and Porcine Heparin Exhibit Comparable Biologic Effects to Referenced Single-Sourced Porcine Heparin |
| title_full | Potency Adjusted Blended Heparin of Bovine, Ovine, and Porcine Heparin Exhibit Comparable Biologic Effects to Referenced Single-Sourced Porcine Heparin |
| title_fullStr | Potency Adjusted Blended Heparin of Bovine, Ovine, and Porcine Heparin Exhibit Comparable Biologic Effects to Referenced Single-Sourced Porcine Heparin |
| title_full_unstemmed | Potency Adjusted Blended Heparin of Bovine, Ovine, and Porcine Heparin Exhibit Comparable Biologic Effects to Referenced Single-Sourced Porcine Heparin |
| title_short | Potency Adjusted Blended Heparin of Bovine, Ovine, and Porcine Heparin Exhibit Comparable Biologic Effects to Referenced Single-Sourced Porcine Heparin |
| title_sort | potency adjusted blended heparin of bovine ovine and porcine heparin exhibit comparable biologic effects to referenced single sourced porcine heparin |
| url | https://doi.org/10.1177/10760296231163251 |
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