A novel in vitro method for the detection and characterization of photosensitizers.
Photoactivation and binding of photoactive chemicals to proteins is a known prerequisite for the formation of immunogenic photoantigens and the induction of photoallergy. The intensive use of products and the availability of new chemicals, along with an increasing exposure to sun light contribute to...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3009729?pdf=render |
| _version_ | 1811277636554457088 |
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| author | Nadine Karschuk Yeliz Tepe Silke Gerlach Wolfgang Pape Horst Wenck Robert Schmucker Klaus-Peter Wittern Andreas Schepky Hendrik Reuter |
| author_facet | Nadine Karschuk Yeliz Tepe Silke Gerlach Wolfgang Pape Horst Wenck Robert Schmucker Klaus-Peter Wittern Andreas Schepky Hendrik Reuter |
| author_sort | Nadine Karschuk |
| collection | DOAJ |
| description | Photoactivation and binding of photoactive chemicals to proteins is a known prerequisite for the formation of immunogenic photoantigens and the induction of photoallergy. The intensive use of products and the availability of new chemicals, along with an increasing exposure to sun light contribute to the risk of photosensitizing adverse reactions. Dendritic cells (DC) play a pivotal role in the induction of allergic contact dermatitis. Human peripheral blood monocyte derived dendritic cells (PBMDC) were thus perceived as an obvious choice for the development of a novel in vitro photosensitization assay using the modulation of cell surface protein expression in response to photosensitizing agents. In this new protocol, known chemicals with photosensitizing, allergenic or non-allergenic potential were pre-incubated with PBMDCs prior to UVA irradiation (1 J/cm(2)). Following a 48 h incubation, the expression of the cell surface molecules CD86, HLA-DR and CD83 was measured by flow cytometry. All tested photosensitizers induced a significant and dose-dependent increase of CD86 expression after irradiation compared to non-irradiated controls. Moreover, the phototoxicity of the chemicals could also be determined. In contrast, (i) CD86 expression was not affected by the chosen irradiation conditions, (ii) increased CD86 expression induced by allergens was independent of irradiation and (iii) no PBMDC activation was observed with the non-allergenic control. The assay proposed here for the evaluation of the photoallergenic potential of chemicals includes the assessment of their allergenic, phototoxic and toxic potential in a single and robust test system and is filling a gap in the in vitro photoallergenicity test battery. |
| first_indexed | 2024-04-13T00:20:27Z |
| format | Article |
| id | doaj.art-a22fb3870067405691136310396f456b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T00:20:27Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a22fb3870067405691136310396f456b2022-12-22T03:10:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-01512e1522110.1371/journal.pone.0015221A novel in vitro method for the detection and characterization of photosensitizers.Nadine KarschukYeliz TepeSilke GerlachWolfgang PapeHorst WenckRobert SchmuckerKlaus-Peter WitternAndreas SchepkyHendrik ReuterPhotoactivation and binding of photoactive chemicals to proteins is a known prerequisite for the formation of immunogenic photoantigens and the induction of photoallergy. The intensive use of products and the availability of new chemicals, along with an increasing exposure to sun light contribute to the risk of photosensitizing adverse reactions. Dendritic cells (DC) play a pivotal role in the induction of allergic contact dermatitis. Human peripheral blood monocyte derived dendritic cells (PBMDC) were thus perceived as an obvious choice for the development of a novel in vitro photosensitization assay using the modulation of cell surface protein expression in response to photosensitizing agents. In this new protocol, known chemicals with photosensitizing, allergenic or non-allergenic potential were pre-incubated with PBMDCs prior to UVA irradiation (1 J/cm(2)). Following a 48 h incubation, the expression of the cell surface molecules CD86, HLA-DR and CD83 was measured by flow cytometry. All tested photosensitizers induced a significant and dose-dependent increase of CD86 expression after irradiation compared to non-irradiated controls. Moreover, the phototoxicity of the chemicals could also be determined. In contrast, (i) CD86 expression was not affected by the chosen irradiation conditions, (ii) increased CD86 expression induced by allergens was independent of irradiation and (iii) no PBMDC activation was observed with the non-allergenic control. The assay proposed here for the evaluation of the photoallergenic potential of chemicals includes the assessment of their allergenic, phototoxic and toxic potential in a single and robust test system and is filling a gap in the in vitro photoallergenicity test battery.http://europepmc.org/articles/PMC3009729?pdf=render |
| spellingShingle | Nadine Karschuk Yeliz Tepe Silke Gerlach Wolfgang Pape Horst Wenck Robert Schmucker Klaus-Peter Wittern Andreas Schepky Hendrik Reuter A novel in vitro method for the detection and characterization of photosensitizers. PLoS ONE |
| title | A novel in vitro method for the detection and characterization of photosensitizers. |
| title_full | A novel in vitro method for the detection and characterization of photosensitizers. |
| title_fullStr | A novel in vitro method for the detection and characterization of photosensitizers. |
| title_full_unstemmed | A novel in vitro method for the detection and characterization of photosensitizers. |
| title_short | A novel in vitro method for the detection and characterization of photosensitizers. |
| title_sort | novel in vitro method for the detection and characterization of photosensitizers |
| url | http://europepmc.org/articles/PMC3009729?pdf=render |
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