First Record of <i>Flavocillium subprimulinum</i> (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against <i>Haemonchus contortus</i> and Protease Activity
This is the first record of the fungus <i>Flavocillium subprimulinum</i> in Mexico. The isolate was taxonomically characterised and cultured in potato dextrose broth (PDB), Czapek–Dox broth (CzDoxB), and sweet potato dextrose broth (SPDB) to obtain its filtrates (FLCF). The nematocidal a...
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MDPI AG
2024-01-01
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| author | Gustavo Pérez-Anzúrez Pedro Mendoza-de Gives Agustín Olmedo-Juárez María Eugenia López-Arellano Génesis Andrea Bautista-García Ana Yuridia Ocampo-Gutiérrez Elke von Son-de Fernex Miguel Ángel Alonso-Díaz Edgar Jesús Delgado-Núñez Adolfo Paz-Silva |
| author_facet | Gustavo Pérez-Anzúrez Pedro Mendoza-de Gives Agustín Olmedo-Juárez María Eugenia López-Arellano Génesis Andrea Bautista-García Ana Yuridia Ocampo-Gutiérrez Elke von Son-de Fernex Miguel Ángel Alonso-Díaz Edgar Jesús Delgado-Núñez Adolfo Paz-Silva |
| author_sort | Gustavo Pérez-Anzúrez |
| collection | DOAJ |
| description | This is the first record of the fungus <i>Flavocillium subprimulinum</i> in Mexico. The isolate was taxonomically characterised and cultured in potato dextrose broth (PDB), Czapek–Dox broth (CzDoxB), and sweet potato dextrose broth (SPDB) to obtain its filtrates (FLCF). The nematocidal activity (NA) of three FLCF concentrations was assessed against <i>Haemonchus contortus</i> L3. Protease activity (PA) was assessed with SDS-PAGE, followed by a zymogram. The NA of the FLCF reached 94.43% in PDB and 95.82% in CzDoxB, respectively, at 100 mg/mL. Lower mortality (64%) was found in SPDB at 100 mg/mL. SDS-PAGE showed bands (in PBS) of ~25, ~40, and ~55 kDa. The zymogram showed protein bands (PBs) with PA in the media, including PBs of ~14, ~40, and ~55 kDa. This study establishes the basis for exploring the potential use of this fungus against <i>H</i>. <i>contortus</i>, which is considered the most pathogenic parasite affecting lambs. |
| first_indexed | 2024-03-08T10:45:31Z |
| format | Article |
| id | doaj.art-a2302e718f97480bae331d7ddf8576c3 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-08T10:45:31Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-a2302e718f97480bae331d7ddf8576c32024-01-26T17:19:07ZengMDPI AGJournal of Fungi2309-608X2024-01-011015610.3390/jof10010056First Record of <i>Flavocillium subprimulinum</i> (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against <i>Haemonchus contortus</i> and Protease ActivityGustavo Pérez-Anzúrez0Pedro Mendoza-de Gives1Agustín Olmedo-Juárez2María Eugenia López-Arellano3Génesis Andrea Bautista-García4Ana Yuridia Ocampo-Gutiérrez5Elke von Son-de Fernex6Miguel Ángel Alonso-Díaz7Edgar Jesús Delgado-Núñez8Adolfo Paz-Silva9Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock-Ministry of Agriculture and Rural Development, (INIFAP-SADER), Jiutepec 62550, MexicoLaboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock-Ministry of Agriculture and Rural Development, (INIFAP-SADER), Jiutepec 62550, MexicoLaboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock-Ministry of Agriculture and Rural Development, (INIFAP-SADER), Jiutepec 62550, MexicoLaboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock-Ministry of Agriculture and Rural Development, (INIFAP-SADER), Jiutepec 62550, MexicoLaboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock-Ministry of Agriculture and Rural Development, (INIFAP-SADER), Jiutepec 62550, MexicoLaboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock-Ministry of Agriculture and Rural Development, (INIFAP-SADER), Jiutepec 62550, MexicoTropical Livestock Center, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Martínez de la Torre 93600, MexicoTropical Livestock Center, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Martínez de la Torre 93600, MexicoFaculty of Agricultural, Livestock and Environmental Sciences, Autonomous University of the State of Guerrero, Iguala de la Independencia 40040, MexicoDepartment of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27142 Lugo, SpainThis is the first record of the fungus <i>Flavocillium subprimulinum</i> in Mexico. The isolate was taxonomically characterised and cultured in potato dextrose broth (PDB), Czapek–Dox broth (CzDoxB), and sweet potato dextrose broth (SPDB) to obtain its filtrates (FLCF). The nematocidal activity (NA) of three FLCF concentrations was assessed against <i>Haemonchus contortus</i> L3. Protease activity (PA) was assessed with SDS-PAGE, followed by a zymogram. The NA of the FLCF reached 94.43% in PDB and 95.82% in CzDoxB, respectively, at 100 mg/mL. Lower mortality (64%) was found in SPDB at 100 mg/mL. SDS-PAGE showed bands (in PBS) of ~25, ~40, and ~55 kDa. The zymogram showed protein bands (PBs) with PA in the media, including PBs of ~14, ~40, and ~55 kDa. This study establishes the basis for exploring the potential use of this fungus against <i>H</i>. <i>contortus</i>, which is considered the most pathogenic parasite affecting lambs.https://www.mdpi.com/2309-608X/10/1/56<i>Flavocillium</i>hypocrealesnematocidal<i>Haemonchus</i>Mexico |
| spellingShingle | Gustavo Pérez-Anzúrez Pedro Mendoza-de Gives Agustín Olmedo-Juárez María Eugenia López-Arellano Génesis Andrea Bautista-García Ana Yuridia Ocampo-Gutiérrez Elke von Son-de Fernex Miguel Ángel Alonso-Díaz Edgar Jesús Delgado-Núñez Adolfo Paz-Silva First Record of <i>Flavocillium subprimulinum</i> (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against <i>Haemonchus contortus</i> and Protease Activity Journal of Fungi <i>Flavocillium</i> hypocreales nematocidal <i>Haemonchus</i> Mexico |
| title | First Record of <i>Flavocillium subprimulinum</i> (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against <i>Haemonchus contortus</i> and Protease Activity |
| title_full | First Record of <i>Flavocillium subprimulinum</i> (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against <i>Haemonchus contortus</i> and Protease Activity |
| title_fullStr | First Record of <i>Flavocillium subprimulinum</i> (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against <i>Haemonchus contortus</i> and Protease Activity |
| title_full_unstemmed | First Record of <i>Flavocillium subprimulinum</i> (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against <i>Haemonchus contortus</i> and Protease Activity |
| title_short | First Record of <i>Flavocillium subprimulinum</i> (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against <i>Haemonchus contortus</i> and Protease Activity |
| title_sort | first record of i flavocillium subprimulinum i cordycipitaceae hypocreales in mexico morphological and molecular characterisation nematocidal activity of its liquid culture filtrates against i haemonchus contortus i and protease activity |
| topic | <i>Flavocillium</i> hypocreales nematocidal <i>Haemonchus</i> Mexico |
| url | https://www.mdpi.com/2309-608X/10/1/56 |
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