Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize
Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought st...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2023-11-01
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| Series: | Stress Biology |
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| Online Access: | https://doi.org/10.1007/s44154-023-00125-x |
| _version_ | 1797578034884116480 |
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| author | Pengyu Zhang Tongchao Wang Liru Cao Zhixin Jiao Lixia Ku Dandan Dou Zhixue Liu Jiaxu Fu Xiaowen Xie Yingfang Zhu Leelyn Chong Li Wei |
| author_facet | Pengyu Zhang Tongchao Wang Liru Cao Zhixin Jiao Lixia Ku Dandan Dou Zhixue Liu Jiaxu Fu Xiaowen Xie Yingfang Zhu Leelyn Chong Li Wei |
| author_sort | Pengyu Zhang |
| collection | DOAJ |
| description | Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response. |
| first_indexed | 2024-03-10T22:17:14Z |
| format | Article |
| id | doaj.art-a2321682af8d40659d3d0be20a0602a9 |
| institution | Directory Open Access Journal |
| issn | 2731-0450 |
| language | English |
| last_indexed | 2024-03-10T22:17:14Z |
| publishDate | 2023-11-01 |
| publisher | Springer |
| record_format | Article |
| series | Stress Biology |
| spelling | doaj.art-a2321682af8d40659d3d0be20a0602a92023-11-19T12:24:22ZengSpringerStress Biology2731-04502023-11-013111510.1007/s44154-023-00125-xMolecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maizePengyu Zhang0Tongchao Wang1Liru Cao2Zhixin Jiao3Lixia Ku4Dandan Dou5Zhixue Liu6Jiaxu Fu7Xiaowen Xie8Yingfang Zhu9Leelyn Chong10Li Wei11National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityNational Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityNational Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityState Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan UniversityNational Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityHenan Academy of Agricultural SciencesNational Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityNational Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityNational Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityState Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan UniversityNational Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityNational Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityAbstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response.https://doi.org/10.1007/s44154-023-00125-xZmRL61R-MYBMYB-related genesDrought stressMaize |
| spellingShingle | Pengyu Zhang Tongchao Wang Liru Cao Zhixin Jiao Lixia Ku Dandan Dou Zhixue Liu Jiaxu Fu Xiaowen Xie Yingfang Zhu Leelyn Chong Li Wei Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize Stress Biology ZmRL6 1R-MYB MYB-related genes Drought stress Maize |
| title | Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize |
| title_full | Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize |
| title_fullStr | Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize |
| title_full_unstemmed | Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize |
| title_short | Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize |
| title_sort | molecular mechanism analysis of zmrl6 positively regulating drought stress tolerance in maize |
| topic | ZmRL6 1R-MYB MYB-related genes Drought stress Maize |
| url | https://doi.org/10.1007/s44154-023-00125-x |
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