Pentamidine-loaded gelatin decreases adhesion formation of flexor tendon
Background: Prevention of adhesion formation following flexor tendon repair is essential for restoration of normal finger function. Although many medications have been studied in the experimental setting to prevent adhesions, clinical application is limited due to the complexity of application and d...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Orthopaedic Translation |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214031X23000815 |
| _version_ | 1797202126736195584 |
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| author | Guidong Shi Nakagawa Koichi Rou Wan Yicun Wang Ramona Reisdorf Abigayle Wilson Tony C.T. Huang Peter C. Amadio Alexander Meves Chunfeng Zhao Steven L. Moran |
| author_facet | Guidong Shi Nakagawa Koichi Rou Wan Yicun Wang Ramona Reisdorf Abigayle Wilson Tony C.T. Huang Peter C. Amadio Alexander Meves Chunfeng Zhao Steven L. Moran |
| author_sort | Guidong Shi |
| collection | DOAJ |
| description | Background: Prevention of adhesion formation following flexor tendon repair is essential for restoration of normal finger function. Although many medications have been studied in the experimental setting to prevent adhesions, clinical application is limited due to the complexity of application and delivery in clinical translation. Methods: In this study, optimal dosages of gelatin and pentamidine were validated by gelatin concentration test. Following cell viability, cell migration, live and dead cell, and cell adhesion assay of the Turkey tenocytes, a model of Turkey tendon repair was established to evaluate the effectiveness of the Pentamidine-Gelatin sheet. Results: Pentamidine carried with gelatin, a Food and drug administration (FDA) approved material for drug delivery, showed good dynamic release, biocompatibility, and degradation. The optimal dose of pentamidine (25ug) was determined in the in vivo study using tenocyte viability, migration, and cell adhesion assays. Further biochemical analyses demonstrated that this positive effect may be due to pentamidine downregulating the Wnt signaling pathway without affecting collagen expression. Conclusions: We tested a FDA-approved antibiotic, pentamidine, for reducing adhesion formation after flexor tendon repair in both in vitro and in vivo using a novel turkey animal model. Compared with the non-pentamidine treatment group, pentamidine treated turkeys had significantly reduced adhesions and improved digit function after six weeks of tendon healing. The translational potential of this article: This study for the first time showed that a common clinical drug, pentamidine, has a potential for clinical application to reduce tendon adhesions and improve tendon gliding function without interfering with tendon healing. |
| first_indexed | 2024-04-24T07:58:29Z |
| format | Article |
| id | doaj.art-a233358cd01c4567869a118bea43c66f |
| institution | Directory Open Access Journal |
| issn | 2214-031X |
| language | English |
| last_indexed | 2024-04-24T07:58:29Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Orthopaedic Translation |
| spelling | doaj.art-a233358cd01c4567869a118bea43c66f2024-04-18T04:20:25ZengElsevierJournal of Orthopaedic Translation2214-031X2024-03-01457587Pentamidine-loaded gelatin decreases adhesion formation of flexor tendonGuidong Shi0Nakagawa Koichi1Rou Wan2Yicun Wang3Ramona Reisdorf4Abigayle Wilson5Tony C.T. Huang6Peter C. Amadio7Alexander Meves8Chunfeng Zhao9Steven L. Moran10Department of Orthopaedics, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China; Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADivision of Plastic Surgery, Department of Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Dermatology, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USA; Corresponding author. Department of Orthopedic Surgery, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.Division of Plastic Surgery, Department of Surgery, Mayo Clinic, Rochester, MN, USA; Corresponding author. Division of Plastic Surgery, Mayo Clinic 200 First Street SW, Rochester, MN, 55905, USA.Background: Prevention of adhesion formation following flexor tendon repair is essential for restoration of normal finger function. Although many medications have been studied in the experimental setting to prevent adhesions, clinical application is limited due to the complexity of application and delivery in clinical translation. Methods: In this study, optimal dosages of gelatin and pentamidine were validated by gelatin concentration test. Following cell viability, cell migration, live and dead cell, and cell adhesion assay of the Turkey tenocytes, a model of Turkey tendon repair was established to evaluate the effectiveness of the Pentamidine-Gelatin sheet. Results: Pentamidine carried with gelatin, a Food and drug administration (FDA) approved material for drug delivery, showed good dynamic release, biocompatibility, and degradation. The optimal dose of pentamidine (25ug) was determined in the in vivo study using tenocyte viability, migration, and cell adhesion assays. Further biochemical analyses demonstrated that this positive effect may be due to pentamidine downregulating the Wnt signaling pathway without affecting collagen expression. Conclusions: We tested a FDA-approved antibiotic, pentamidine, for reducing adhesion formation after flexor tendon repair in both in vitro and in vivo using a novel turkey animal model. Compared with the non-pentamidine treatment group, pentamidine treated turkeys had significantly reduced adhesions and improved digit function after six weeks of tendon healing. The translational potential of this article: This study for the first time showed that a common clinical drug, pentamidine, has a potential for clinical application to reduce tendon adhesions and improve tendon gliding function without interfering with tendon healing.http://www.sciencedirect.com/science/article/pii/S2214031X23000815AdhesionFlexor tendon repairGelatinPentamidineTurkey model |
| spellingShingle | Guidong Shi Nakagawa Koichi Rou Wan Yicun Wang Ramona Reisdorf Abigayle Wilson Tony C.T. Huang Peter C. Amadio Alexander Meves Chunfeng Zhao Steven L. Moran Pentamidine-loaded gelatin decreases adhesion formation of flexor tendon Journal of Orthopaedic Translation Adhesion Flexor tendon repair Gelatin Pentamidine Turkey model |
| title | Pentamidine-loaded gelatin decreases adhesion formation of flexor tendon |
| title_full | Pentamidine-loaded gelatin decreases adhesion formation of flexor tendon |
| title_fullStr | Pentamidine-loaded gelatin decreases adhesion formation of flexor tendon |
| title_full_unstemmed | Pentamidine-loaded gelatin decreases adhesion formation of flexor tendon |
| title_short | Pentamidine-loaded gelatin decreases adhesion formation of flexor tendon |
| title_sort | pentamidine loaded gelatin decreases adhesion formation of flexor tendon |
| topic | Adhesion Flexor tendon repair Gelatin Pentamidine Turkey model |
| url | http://www.sciencedirect.com/science/article/pii/S2214031X23000815 |
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