Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri Lanka
Background: Haemolysis – one of the major limiting factors of red cell concentrate quality – must be measured as a quality-monitoring requirement. According to international quality standards, percentage haemolysis must be monitored in 1.0% of red cell concentrates produced monthly and maintained un...
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AOSIS
2023-02-01
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| Series: | African Journal of Laboratory Medicine |
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| Online Access: | https://ajlmonline.org/index.php/ajlm/article/view/1987 |
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| author | Caroline A. Fernando Deklanji T. Dissanayake Uththara I. Hewamana Shyamini Rathnaweera Wickrama A. Samanthilake Ranga Tudugala Kithsiri B. Jayasekara Kumudu Kuruppu |
| author_facet | Caroline A. Fernando Deklanji T. Dissanayake Uththara I. Hewamana Shyamini Rathnaweera Wickrama A. Samanthilake Ranga Tudugala Kithsiri B. Jayasekara Kumudu Kuruppu |
| author_sort | Caroline A. Fernando |
| collection | DOAJ |
| description | Background: Haemolysis – one of the major limiting factors of red cell concentrate quality – must be measured as a quality-monitoring requirement. According to international quality standards, percentage haemolysis must be monitored in 1.0% of red cell concentrates produced monthly and maintained under 0.8%.
Objective: This study assessed three alternative methods for determining plasma haemoglobin concentration in peripheral blood banks that lack a plasma or low haemoglobin photometer – the gold-standard method – in Sri Lanka.
Methods: A standard haemolysate was prepared using an unexpired whole blood pack of normal haemoglobin concentration. A concentration series from 0.1 g/dL to 1.0 g/dL was prepared by diluting portions of standard haemolysate with saline. The alternative methods, namely visual haemoglobin colour scale, spectrophotometric calibration graph, and standard haemolysate capillary tube comparison, were designed using this concentration series and were used to test red cell concentrates received at the Quality Control Department of the National Blood Center, Sri Lanka, from February 2021 to May 2021.
Results: A strong correlation was observed between the haemoglobin photometer method and the alternative methods (R = ~0.9). Based on the linear regression model, the standard haemolysate capillary tube comparison method was the best of the three alternative methods (R2 = 0.974).
Conclusion: All three alternative methods are recommended for use in peripheral blood banks. The standard haemolysate capillary tube comparison method was the best model. |
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| series | African Journal of Laboratory Medicine |
| spelling | doaj.art-a2351eca56344dc4a456dd9233656ffc2023-03-02T11:44:33ZengAOSISAfrican Journal of Laboratory Medicine2225-20022225-20102023-02-01121e1e710.4102/ajlm.v12i1.1987420Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri LankaCaroline A. Fernando0Deklanji T. Dissanayake1Uththara I. Hewamana2Shyamini Rathnaweera3Wickrama A. Samanthilake4Ranga Tudugala5Kithsiri B. Jayasekara6Kumudu Kuruppu7Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, WeraheraDepartment of Medical Laboratory Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, WeraheraDepartment of Medical Laboratory Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, WeraheraDepartment of Medical Laboratory Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, WeraheraDepartment of Quality Management, National Blood Center, NarahenpitaDepartment of Radiography and Radiotherapy, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, WeraheraDepartment of Radiography and Radiotherapy, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, WeraheraDepartment of Quality Management, National Blood Center, NarahenpitaBackground: Haemolysis – one of the major limiting factors of red cell concentrate quality – must be measured as a quality-monitoring requirement. According to international quality standards, percentage haemolysis must be monitored in 1.0% of red cell concentrates produced monthly and maintained under 0.8%. Objective: This study assessed three alternative methods for determining plasma haemoglobin concentration in peripheral blood banks that lack a plasma or low haemoglobin photometer – the gold-standard method – in Sri Lanka. Methods: A standard haemolysate was prepared using an unexpired whole blood pack of normal haemoglobin concentration. A concentration series from 0.1 g/dL to 1.0 g/dL was prepared by diluting portions of standard haemolysate with saline. The alternative methods, namely visual haemoglobin colour scale, spectrophotometric calibration graph, and standard haemolysate capillary tube comparison, were designed using this concentration series and were used to test red cell concentrates received at the Quality Control Department of the National Blood Center, Sri Lanka, from February 2021 to May 2021. Results: A strong correlation was observed between the haemoglobin photometer method and the alternative methods (R = ~0.9). Based on the linear regression model, the standard haemolysate capillary tube comparison method was the best of the three alternative methods (R2 = 0.974). Conclusion: All three alternative methods are recommended for use in peripheral blood banks. The standard haemolysate capillary tube comparison method was the best model.https://ajlmonline.org/index.php/ajlm/article/view/1987blood bankscapillary tube comparisonhaemoglobin colour scalepercentage haemolysisred cell concentrate |
| spellingShingle | Caroline A. Fernando Deklanji T. Dissanayake Uththara I. Hewamana Shyamini Rathnaweera Wickrama A. Samanthilake Ranga Tudugala Kithsiri B. Jayasekara Kumudu Kuruppu Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri Lanka African Journal of Laboratory Medicine blood banks capillary tube comparison haemoglobin colour scale percentage haemolysis red cell concentrate |
| title | Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri Lanka |
| title_full | Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri Lanka |
| title_fullStr | Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri Lanka |
| title_full_unstemmed | Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri Lanka |
| title_short | Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri Lanka |
| title_sort | alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in sri lanka |
| topic | blood banks capillary tube comparison haemoglobin colour scale percentage haemolysis red cell concentrate |
| url | https://ajlmonline.org/index.php/ajlm/article/view/1987 |
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