Recovery of 2R.2S<sup>k</sup> Triticale-<i>Aegilops kotschyi</i> Robertsonian Chromosome Translocations
Robertsonian translocations (RobTs) in the progeny of triticale (×<i>Triticosecale</i> Wittmack) plants with monosomic substitution of <i>Aegilops kotschyi</i> chromosome 2S<sup>k</sup> (2R) were investigated by fluorescence in-situ hybridization. Chromos...
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MDPI AG
2019-10-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/9/10/646 |
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| author | Waldemar Ulaszewski Jolanta Belter Halina Wiśniewska Joanna Szymczak Roksana Skowrońska Dylan Phillips Michał T. Kwiatek |
| author_facet | Waldemar Ulaszewski Jolanta Belter Halina Wiśniewska Joanna Szymczak Roksana Skowrońska Dylan Phillips Michał T. Kwiatek |
| author_sort | Waldemar Ulaszewski |
| collection | DOAJ |
| description | Robertsonian translocations (RobTs) in the progeny of triticale (×<i>Triticosecale</i> Wittmack) plants with monosomic substitution of <i>Aegilops kotschyi</i> chromosome 2S<sup>k</sup> (2R) were investigated by fluorescence in-situ hybridization. Chromosome 2S<sup>k</sup> of <i>Ae. kotschyi</i> is reported to possess many valuable loci, such as <i>Lr54 + Yr37</i> leaf and stripe (yellow) rust resistance genes. We used a standard procedure to produce RobTs, which consisted of self-pollination of monosomic triticale plants, carrying 2R and 2S<sup>k</sup> chromosomes in monosomic condition. This approach did not result in RobTs. Simultaneously, we succeeded in producing 11 plants carrying 2R.2S<sup>k</sup> compensatory RobTs using an alternative approach that utilized ditelosomic lines of triticale carrying 2RS (short arm) and 2RL (long arm) telosomic chromosomes. Identification of molecular markers linked to <i>Lr54 + Yr37</i> genes in the translocation plants confirmed that these resources can be exploited in current triticale breeding programmes. |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-12-14T00:36:28Z |
| publishDate | 2019-10-01 |
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| spelling | doaj.art-a2385a863e944d9dbc41d33887ee82be2022-12-21T23:24:38ZengMDPI AGAgronomy2073-43952019-10-0191064610.3390/agronomy9100646agronomy9100646Recovery of 2R.2S<sup>k</sup> Triticale-<i>Aegilops kotschyi</i> Robertsonian Chromosome TranslocationsWaldemar Ulaszewski0Jolanta Belter1Halina Wiśniewska2Joanna Szymczak3Roksana Skowrońska4Dylan Phillips5Michał T. Kwiatek6Department of Genomics, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34; 60-479 Poznań, PolandDepartment of Genomics, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34; 60-479 Poznań, PolandDepartment of Genomics, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34; 60-479 Poznań, PolandDepartment of Genetics and Plant Breeding, Poznań University of Life Sciences, Dojazd 11; 60-632 Poznań, PolandDepartment of Genetics and Plant Breeding, Poznań University of Life Sciences, Dojazd 11; 60-632 Poznań, PolandInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Ceredigion, Wales SY23 3DA, UKDepartment of Genomics, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34; 60-479 Poznań, PolandRobertsonian translocations (RobTs) in the progeny of triticale (×<i>Triticosecale</i> Wittmack) plants with monosomic substitution of <i>Aegilops kotschyi</i> chromosome 2S<sup>k</sup> (2R) were investigated by fluorescence in-situ hybridization. Chromosome 2S<sup>k</sup> of <i>Ae. kotschyi</i> is reported to possess many valuable loci, such as <i>Lr54 + Yr37</i> leaf and stripe (yellow) rust resistance genes. We used a standard procedure to produce RobTs, which consisted of self-pollination of monosomic triticale plants, carrying 2R and 2S<sup>k</sup> chromosomes in monosomic condition. This approach did not result in RobTs. Simultaneously, we succeeded in producing 11 plants carrying 2R.2S<sup>k</sup> compensatory RobTs using an alternative approach that utilized ditelosomic lines of triticale carrying 2RS (short arm) and 2RL (long arm) telosomic chromosomes. Identification of molecular markers linked to <i>Lr54 + Yr37</i> genes in the translocation plants confirmed that these resources can be exploited in current triticale breeding programmes.https://www.mdpi.com/2073-4395/9/10/646<i>aegilops</i>centric breakschromosome fusionrobertsonian translocationstelosomic chromosomestriticale |
| spellingShingle | Waldemar Ulaszewski Jolanta Belter Halina Wiśniewska Joanna Szymczak Roksana Skowrońska Dylan Phillips Michał T. Kwiatek Recovery of 2R.2S<sup>k</sup> Triticale-<i>Aegilops kotschyi</i> Robertsonian Chromosome Translocations Agronomy <i>aegilops</i> centric breaks chromosome fusion robertsonian translocations telosomic chromosomes triticale |
| title | Recovery of 2R.2S<sup>k</sup> Triticale-<i>Aegilops kotschyi</i> Robertsonian Chromosome Translocations |
| title_full | Recovery of 2R.2S<sup>k</sup> Triticale-<i>Aegilops kotschyi</i> Robertsonian Chromosome Translocations |
| title_fullStr | Recovery of 2R.2S<sup>k</sup> Triticale-<i>Aegilops kotschyi</i> Robertsonian Chromosome Translocations |
| title_full_unstemmed | Recovery of 2R.2S<sup>k</sup> Triticale-<i>Aegilops kotschyi</i> Robertsonian Chromosome Translocations |
| title_short | Recovery of 2R.2S<sup>k</sup> Triticale-<i>Aegilops kotschyi</i> Robertsonian Chromosome Translocations |
| title_sort | recovery of 2r 2s sup k sup triticale i aegilops kotschyi i robertsonian chromosome translocations |
| topic | <i>aegilops</i> centric breaks chromosome fusion robertsonian translocations telosomic chromosomes triticale |
| url | https://www.mdpi.com/2073-4395/9/10/646 |
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