Of monkeys and men: immunomic profiling of sera from humans and non-human primates resistant to schistosomiasis reveals novel potential vaccine candidates
Schistosoma haematobium affects more than 100 million people throughout Africa and is the causative agent of urogenital schistosomiasis. The parasite is strongly associated with urothelial cancer in infected individuals and as such is designated a group I carcinogen by the International Agency for R...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-05-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00213/full |
| _version_ | 1819141315882909696 |
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| author | Mark ePearson Luke eBecker Patrick eDriguez Neil David Young Soraya eGaze Tiago eMendes Xiao-Hong eLi Denise eDoolan Nicholas eMidzi Takafira eMduluza Donald eMcManus Alan eWilson Jeffrey eBethony Norman eNausch Francisca eMutapi Philip eFelgner Alex eLoukas |
| author_facet | Mark ePearson Luke eBecker Patrick eDriguez Neil David Young Soraya eGaze Tiago eMendes Xiao-Hong eLi Denise eDoolan Nicholas eMidzi Takafira eMduluza Donald eMcManus Alan eWilson Jeffrey eBethony Norman eNausch Francisca eMutapi Philip eFelgner Alex eLoukas |
| author_sort | Mark ePearson |
| collection | DOAJ |
| description | Schistosoma haematobium affects more than 100 million people throughout Africa and is the causative agent of urogenital schistosomiasis. The parasite is strongly associated with urothelial cancer in infected individuals and as such is designated a group I carcinogen by the International Agency for Research on Cancer. Using a protein microarray containing schistosome proteins, we sought to identify antigens that were the targets of protective IgG1 immune responses in S. haematobium-exposed individuals that acquire drug-induced resistance (DIR) to schistosomiasis after praziquantel treatment. Numerous antigens with known vaccine potential were identified, including calpain (Smp80), tetraspanins, glutathione-S-transferases and glucose transporters (SGTP1), as well as previously uncharacterized proteins. Reactive IgG1 responses were not elevated in exposed individuals who did not acquire DIR. To complement our human subjects study, we screened for antigen targets of rhesus macaques rendered resistant to Schistosoma japonicum by experimental infection followed by self-cure, and discovered a number of new and known vaccine targets, including major targets recognised by our human subjects. This study has further validated the immunomics-based approach to schistosomiasis vaccine antigen discovery and identified numerous novel potential vaccine antigens. |
| first_indexed | 2024-12-22T11:52:30Z |
| format | Article |
| id | doaj.art-a238c7916d964a99a8863ddbd74a14e9 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T11:52:30Z |
| publishDate | 2015-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-a238c7916d964a99a8863ddbd74a14e92022-12-21T18:26:57ZengFrontiers Media S.A.Frontiers in Immunology1664-32242015-05-01610.3389/fimmu.2015.00213144353Of monkeys and men: immunomic profiling of sera from humans and non-human primates resistant to schistosomiasis reveals novel potential vaccine candidatesMark ePearson0Luke eBecker1Patrick eDriguez2Neil David Young3Soraya eGaze4Tiago eMendes5Xiao-Hong eLi6Denise eDoolan7Nicholas eMidzi8Takafira eMduluza9Donald eMcManus10Alan eWilson11Jeffrey eBethony12Norman eNausch13Francisca eMutapi14Philip eFelgner15Alex eLoukas16James Cook UniversityJames Cook UniversityQIMR Berghofer Medical Research InstituteUniversity of MelbourneFederal University of Minas GeraisFederal University of Minas GeraisNational Institute of Parasitic DiseasesQIMR Berghofer Medical Research InstituteNational Institutes of Health ResearchUniversity of ZimbabweQIMR Berghofer Medical Research InstituteUniversity of YorkGeorge Washington UniversityUniversity of EdinburghUniversity of EdinburghUniversity of California, IrvineJames Cook UniversitySchistosoma haematobium affects more than 100 million people throughout Africa and is the causative agent of urogenital schistosomiasis. The parasite is strongly associated with urothelial cancer in infected individuals and as such is designated a group I carcinogen by the International Agency for Research on Cancer. Using a protein microarray containing schistosome proteins, we sought to identify antigens that were the targets of protective IgG1 immune responses in S. haematobium-exposed individuals that acquire drug-induced resistance (DIR) to schistosomiasis after praziquantel treatment. Numerous antigens with known vaccine potential were identified, including calpain (Smp80), tetraspanins, glutathione-S-transferases and glucose transporters (SGTP1), as well as previously uncharacterized proteins. Reactive IgG1 responses were not elevated in exposed individuals who did not acquire DIR. To complement our human subjects study, we screened for antigen targets of rhesus macaques rendered resistant to Schistosoma japonicum by experimental infection followed by self-cure, and discovered a number of new and known vaccine targets, including major targets recognised by our human subjects. This study has further validated the immunomics-based approach to schistosomiasis vaccine antigen discovery and identified numerous novel potential vaccine antigens.http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00213/fullSchistosomiasishumanVaccineprotein microarraydrug-induced resistance |
| spellingShingle | Mark ePearson Luke eBecker Patrick eDriguez Neil David Young Soraya eGaze Tiago eMendes Xiao-Hong eLi Denise eDoolan Nicholas eMidzi Takafira eMduluza Donald eMcManus Alan eWilson Jeffrey eBethony Norman eNausch Francisca eMutapi Philip eFelgner Alex eLoukas Of monkeys and men: immunomic profiling of sera from humans and non-human primates resistant to schistosomiasis reveals novel potential vaccine candidates Frontiers in Immunology Schistosomiasis human Vaccine protein microarray drug-induced resistance |
| title | Of monkeys and men: immunomic profiling of sera from humans and non-human primates resistant to schistosomiasis reveals novel potential vaccine candidates |
| title_full | Of monkeys and men: immunomic profiling of sera from humans and non-human primates resistant to schistosomiasis reveals novel potential vaccine candidates |
| title_fullStr | Of monkeys and men: immunomic profiling of sera from humans and non-human primates resistant to schistosomiasis reveals novel potential vaccine candidates |
| title_full_unstemmed | Of monkeys and men: immunomic profiling of sera from humans and non-human primates resistant to schistosomiasis reveals novel potential vaccine candidates |
| title_short | Of monkeys and men: immunomic profiling of sera from humans and non-human primates resistant to schistosomiasis reveals novel potential vaccine candidates |
| title_sort | of monkeys and men immunomic profiling of sera from humans and non human primates resistant to schistosomiasis reveals novel potential vaccine candidates |
| topic | Schistosomiasis human Vaccine protein microarray drug-induced resistance |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00213/full |
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