Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor model
Summary: Human killer cell immunoglobulin-like receptor, three Ig domains and long cytoplasmic tail (KIR3DL3) is expressed on natural killer (NK) cells and is a newly identified inhibitory receptor for B7 family member HERV-H LTR-associating protein 2 (HHLA2). Here, we summarize the isolation and ex...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006980 |
| _version_ | 1811199460198318080 |
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| author | Xiaoxin Ren Devin T. Corrigan Xingxing Zang |
| author_facet | Xiaoxin Ren Devin T. Corrigan Xingxing Zang |
| author_sort | Xiaoxin Ren |
| collection | DOAJ |
| description | Summary: Human killer cell immunoglobulin-like receptor, three Ig domains and long cytoplasmic tail (KIR3DL3) is expressed on natural killer (NK) cells and is a newly identified inhibitory receptor for B7 family member HERV-H LTR-associating protein 2 (HHLA2). Here, we summarize the isolation and expansion of KIR3DL3+ human NK cells, and in vitro functional characterization of in-house anti-KIR3DL3 monoclonal antibody (mAb). We also describe a human NK cell-based xenogeneic lung tumor model for testing the therapeutic activity of KIR3DL3 blockade in vivo.For complete details on the use and execution of this protocol, please refer to Wei et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-12T01:48:34Z |
| format | Article |
| id | doaj.art-a23d725b356441379a18bedea1fc54bf |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-12T01:48:34Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-a23d725b356441379a18bedea1fc54bf2022-12-22T03:53:00ZengElsevierSTAR Protocols2666-16672022-12-0134101818Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor modelXiaoxin Ren0Devin T. Corrigan1Xingxing Zang2Department of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USADepartment of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USADepartment of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Oncology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Urology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Corresponding authorSummary: Human killer cell immunoglobulin-like receptor, three Ig domains and long cytoplasmic tail (KIR3DL3) is expressed on natural killer (NK) cells and is a newly identified inhibitory receptor for B7 family member HERV-H LTR-associating protein 2 (HHLA2). Here, we summarize the isolation and expansion of KIR3DL3+ human NK cells, and in vitro functional characterization of in-house anti-KIR3DL3 monoclonal antibody (mAb). We also describe a human NK cell-based xenogeneic lung tumor model for testing the therapeutic activity of KIR3DL3 blockade in vivo.For complete details on the use and execution of this protocol, please refer to Wei et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006980Cell BiologyCell isolationCell-based AssaysCancerHealth SciencesImmunology |
| spellingShingle | Xiaoxin Ren Devin T. Corrigan Xingxing Zang Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor model STAR Protocols Cell Biology Cell isolation Cell-based Assays Cancer Health Sciences Immunology |
| title | Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor model |
| title_full | Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor model |
| title_fullStr | Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor model |
| title_full_unstemmed | Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor model |
| title_short | Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor model |
| title_sort | protocol for evaluating antitumor activity of kir3dl3 blockade in an nk cell based xenogeneic lung tumor model |
| topic | Cell Biology Cell isolation Cell-based Assays Cancer Health Sciences Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006980 |
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