Non-Destructive Monitoring via Electrochemical NADH Detection in Murine Cells
Nicotinamide adenine dinucleotide (NADH) is an important cofactor involved in metabolic redox reactions in living cells. The detection of NADH in living animal cells is a challenge. We developed a one-step monitoring method for NADH via an electrocatalytic reaction that uses a surface-modified, scre...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/2/107 |
| _version_ | 1797482218515333120 |
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| author | Ju Kyung Lee Han Na Suh Sung Hoon Yoon Kyu Hong Lee Sae Young Ahn Hyung Jin Kim Sang Hee Kim |
| author_facet | Ju Kyung Lee Han Na Suh Sung Hoon Yoon Kyu Hong Lee Sae Young Ahn Hyung Jin Kim Sang Hee Kim |
| author_sort | Ju Kyung Lee |
| collection | DOAJ |
| description | Nicotinamide adenine dinucleotide (NADH) is an important cofactor involved in metabolic redox reactions in living cells. The detection of NADH in living animal cells is a challenge. We developed a one-step monitoring method for NADH via an electrocatalytic reaction that uses a surface-modified, screen-printed electrode (SPE) having a redox active monolayer 4′-mercapto-N-phenlyquinone diamine (NPQD) formed by a self-assembled monolayer (SAM) of an aromatic thiol, 4-aminothiophenol (4-ATP). This electrode has a limit of detection (LOD) of 0.49 μM and a sensitivity of 0.0076 ± 0.0006 μM/μA in cell culture media, which indicates that it retains its selectivity. The applicability of this NADH sensor was demonstrated for the first time by cell viability monitoring via NADH-sensing in cell culture supernatants. |
| first_indexed | 2024-03-09T22:29:09Z |
| format | Article |
| id | doaj.art-a23e0fda2df348248e5432342b941532 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T22:29:09Z |
| publishDate | 2022-02-01 |
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| series | Biosensors |
| spelling | doaj.art-a23e0fda2df348248e5432342b9415322023-11-23T19:01:26ZengMDPI AGBiosensors2079-63742022-02-0112210710.3390/bios12020107Non-Destructive Monitoring via Electrochemical NADH Detection in Murine CellsJu Kyung Lee0Han Na Suh1Sung Hoon Yoon2Kyu Hong Lee3Sae Young Ahn4Hyung Jin Kim5Sang Hee Kim6Department of Medical IT Convergence, Kumoh National Institute of Technology, Gumi 39177, KoreaKorea Institute of Toxicology, Jeongeup 56212, KoreaKorea Institute of Toxicology, Jeongeup 56212, KoreaKorea Institute of Toxicology, Jeongeup 56212, KoreaNDD Inc., Gumi 39253, KoreaDigital Health Care Research Center, Gumi Electronics and Information Technology Research Institute (GERI), Gumi 39253, KoreaDepartment of Medical IT Convergence, Kumoh National Institute of Technology, Gumi 39177, KoreaNicotinamide adenine dinucleotide (NADH) is an important cofactor involved in metabolic redox reactions in living cells. The detection of NADH in living animal cells is a challenge. We developed a one-step monitoring method for NADH via an electrocatalytic reaction that uses a surface-modified, screen-printed electrode (SPE) having a redox active monolayer 4′-mercapto-N-phenlyquinone diamine (NPQD) formed by a self-assembled monolayer (SAM) of an aromatic thiol, 4-aminothiophenol (4-ATP). This electrode has a limit of detection (LOD) of 0.49 μM and a sensitivity of 0.0076 ± 0.0006 μM/μA in cell culture media, which indicates that it retains its selectivity. The applicability of this NADH sensor was demonstrated for the first time by cell viability monitoring via NADH-sensing in cell culture supernatants.https://www.mdpi.com/2079-6374/12/2/107electrochemical amperometrysurface modificationscreen-printed electrode (SPE)polyhexamethylene guanidine-phosphate (PHMG-p)continuous monitoring |
| spellingShingle | Ju Kyung Lee Han Na Suh Sung Hoon Yoon Kyu Hong Lee Sae Young Ahn Hyung Jin Kim Sang Hee Kim Non-Destructive Monitoring via Electrochemical NADH Detection in Murine Cells Biosensors electrochemical amperometry surface modification screen-printed electrode (SPE) polyhexamethylene guanidine-phosphate (PHMG-p) continuous monitoring |
| title | Non-Destructive Monitoring via Electrochemical NADH Detection in Murine Cells |
| title_full | Non-Destructive Monitoring via Electrochemical NADH Detection in Murine Cells |
| title_fullStr | Non-Destructive Monitoring via Electrochemical NADH Detection in Murine Cells |
| title_full_unstemmed | Non-Destructive Monitoring via Electrochemical NADH Detection in Murine Cells |
| title_short | Non-Destructive Monitoring via Electrochemical NADH Detection in Murine Cells |
| title_sort | non destructive monitoring via electrochemical nadh detection in murine cells |
| topic | electrochemical amperometry surface modification screen-printed electrode (SPE) polyhexamethylene guanidine-phosphate (PHMG-p) continuous monitoring |
| url | https://www.mdpi.com/2079-6374/12/2/107 |
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