Antioxidant and Neuroprotective Potential of the Brown Seaweed <i>Bifurcaria bifurcata</i> in an in vitro Parkinson’s Disease Model
<i>Bifurcaria bifurcata</i> is a marine brown seaweed mainly found on the Atlantic coast. Herein, we report the antioxidant and neuroprotective activities of seven fractions (F1⁻F7) obtained by normal phase chromatography from the <i>B. bifurcata</i> dichloromethane...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/2/85 |
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| author | Joana Silva Celso Alves Rafaela Freitas Alice Martins Susete Pinteus Joana Ribeiro Helena Gaspar Amparo Alfonso Rui Pedrosa |
| author_facet | Joana Silva Celso Alves Rafaela Freitas Alice Martins Susete Pinteus Joana Ribeiro Helena Gaspar Amparo Alfonso Rui Pedrosa |
| author_sort | Joana Silva |
| collection | DOAJ |
| description | <i>Bifurcaria bifurcata</i> is a marine brown seaweed mainly found on the Atlantic coast. Herein, we report the antioxidant and neuroprotective activities of seven fractions (F1⁻F7) obtained by normal phase chromatography from the <i>B. bifurcata</i> dichloromethane extract, as well as of its two major isolated diterpenes. Total phenolic content of fractions was determined by the Folin⁻Ciocalteu method, while antioxidant activity was evaluated by the DPPH, ORAC, and FRAP assays. Neuroprotective effects were evaluated in a neurotoxic model induced by 6-hydroxydopamine (6-OHDA) in a human neuroblastoma cell line (SH-SY5Y), while the mechanisms associated to neuroprotection were investigated by the determination of mitochondrial membrane potential, H<sub>2</sub>O<sub>2</sub> production, Caspase-3 activity, and by observation of DNA fragmentation. Fractions F4 and F5 exhibited the best neuroprotective and antioxidant activities, respectively. F4 fraction prevented changes in mitochondrial potential, and induced a reduction of H<sub>2</sub>O<sub>2</sub> levels production and an increase in cell viability, suggesting that it may contain multi-target compounds acting on different pathways. Hence, this fraction was subjected to purification steps, affording the known diterpenes eleganolone and eleganonal. Both compounds exhibited antioxidant potential, being interesting candidates for further neuroprotective studies. |
| first_indexed | 2024-04-13T07:21:22Z |
| format | Article |
| id | doaj.art-a23f7d16d5714c93a25531da40552983 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T07:21:22Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a23f7d16d5714c93a25531da405529832022-12-22T02:56:37ZengMDPI AGMarine Drugs1660-33972019-02-011728510.3390/md17020085md17020085Antioxidant and Neuroprotective Potential of the Brown Seaweed <i>Bifurcaria bifurcata</i> in an in vitro Parkinson’s Disease ModelJoana Silva0Celso Alves1Rafaela Freitas2Alice Martins3Susete Pinteus4Joana Ribeiro5Helena Gaspar6Amparo Alfonso7Rui Pedrosa8MARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, PortugalDepartment of Pharmacology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainMARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, Portugal<i>Bifurcaria bifurcata</i> is a marine brown seaweed mainly found on the Atlantic coast. Herein, we report the antioxidant and neuroprotective activities of seven fractions (F1⁻F7) obtained by normal phase chromatography from the <i>B. bifurcata</i> dichloromethane extract, as well as of its two major isolated diterpenes. Total phenolic content of fractions was determined by the Folin⁻Ciocalteu method, while antioxidant activity was evaluated by the DPPH, ORAC, and FRAP assays. Neuroprotective effects were evaluated in a neurotoxic model induced by 6-hydroxydopamine (6-OHDA) in a human neuroblastoma cell line (SH-SY5Y), while the mechanisms associated to neuroprotection were investigated by the determination of mitochondrial membrane potential, H<sub>2</sub>O<sub>2</sub> production, Caspase-3 activity, and by observation of DNA fragmentation. Fractions F4 and F5 exhibited the best neuroprotective and antioxidant activities, respectively. F4 fraction prevented changes in mitochondrial potential, and induced a reduction of H<sub>2</sub>O<sub>2</sub> levels production and an increase in cell viability, suggesting that it may contain multi-target compounds acting on different pathways. Hence, this fraction was subjected to purification steps, affording the known diterpenes eleganolone and eleganonal. Both compounds exhibited antioxidant potential, being interesting candidates for further neuroprotective studies.https://www.mdpi.com/1660-3397/17/2/856-hydroxydopamineseaweedsSH-SY5Y cellsneuroprotectionmitochondrial membrane potentialCaspase-3 activityeleganoloneeleganonalneurodegenerative diseases |
| spellingShingle | Joana Silva Celso Alves Rafaela Freitas Alice Martins Susete Pinteus Joana Ribeiro Helena Gaspar Amparo Alfonso Rui Pedrosa Antioxidant and Neuroprotective Potential of the Brown Seaweed <i>Bifurcaria bifurcata</i> in an in vitro Parkinson’s Disease Model Marine Drugs 6-hydroxydopamine seaweeds SH-SY5Y cells neuroprotection mitochondrial membrane potential Caspase-3 activity eleganolone eleganonal neurodegenerative diseases |
| title | Antioxidant and Neuroprotective Potential of the Brown Seaweed <i>Bifurcaria bifurcata</i> in an in vitro Parkinson’s Disease Model |
| title_full | Antioxidant and Neuroprotective Potential of the Brown Seaweed <i>Bifurcaria bifurcata</i> in an in vitro Parkinson’s Disease Model |
| title_fullStr | Antioxidant and Neuroprotective Potential of the Brown Seaweed <i>Bifurcaria bifurcata</i> in an in vitro Parkinson’s Disease Model |
| title_full_unstemmed | Antioxidant and Neuroprotective Potential of the Brown Seaweed <i>Bifurcaria bifurcata</i> in an in vitro Parkinson’s Disease Model |
| title_short | Antioxidant and Neuroprotective Potential of the Brown Seaweed <i>Bifurcaria bifurcata</i> in an in vitro Parkinson’s Disease Model |
| title_sort | antioxidant and neuroprotective potential of the brown seaweed i bifurcaria bifurcata i in an in vitro parkinson s disease model |
| topic | 6-hydroxydopamine seaweeds SH-SY5Y cells neuroprotection mitochondrial membrane potential Caspase-3 activity eleganolone eleganonal neurodegenerative diseases |
| url | https://www.mdpi.com/1660-3397/17/2/85 |
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