Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation
Lactic acid bacteria (LAB) are the primary genera of the intestinal flora and have many probiotic functions. In the present study, Lactobacillus rhamnosus GG (LGG) ATCC 53103 was used to treat BALB/c mice. After LGG intervention, both low and high LGG doses were shown to improve the observed OTU, Ch...
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.607735/full |
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| author | Chun-wei Shi Chun-wei Shi Chun-wei Shi Ming-yang Cheng Ming-yang Cheng Ming-yang Cheng Xin Yang Xin Yang Xin Yang Yi-yuan Lu Yi-yuan Lu Yi-yuan Lu Hong-duo Yin Hong-duo Yin Hong-duo Yin Yan Zeng Yan Zeng Yan Zeng Ru-yu Wang Ru-yu Wang Ru-yu Wang Yan-long Jiang Yan-long Jiang Yan-long Jiang Wen-tao Yang Wen-tao Yang Wen-tao Yang Jian-zhong Wang Jian-zhong Wang Jian-zhong Wang Dan-dan Zhao Dan-dan Zhao Dan-dan Zhao Hai-bin Huang Hai-bin Huang Hai-bin Huang Li-ping Ye Li-ping Ye Li-ping Ye Xin Cao Xin Cao Xin Cao Gui-lian Yang Gui-lian Yang Gui-lian Yang Chun-feng Wang Chun-feng Wang Chun-feng Wang |
| author_facet | Chun-wei Shi Chun-wei Shi Chun-wei Shi Ming-yang Cheng Ming-yang Cheng Ming-yang Cheng Xin Yang Xin Yang Xin Yang Yi-yuan Lu Yi-yuan Lu Yi-yuan Lu Hong-duo Yin Hong-duo Yin Hong-duo Yin Yan Zeng Yan Zeng Yan Zeng Ru-yu Wang Ru-yu Wang Ru-yu Wang Yan-long Jiang Yan-long Jiang Yan-long Jiang Wen-tao Yang Wen-tao Yang Wen-tao Yang Jian-zhong Wang Jian-zhong Wang Jian-zhong Wang Dan-dan Zhao Dan-dan Zhao Dan-dan Zhao Hai-bin Huang Hai-bin Huang Hai-bin Huang Li-ping Ye Li-ping Ye Li-ping Ye Xin Cao Xin Cao Xin Cao Gui-lian Yang Gui-lian Yang Gui-lian Yang Chun-feng Wang Chun-feng Wang Chun-feng Wang |
| author_sort | Chun-wei Shi |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) are the primary genera of the intestinal flora and have many probiotic functions. In the present study, Lactobacillus rhamnosus GG (LGG) ATCC 53103 was used to treat BALB/c mice. After LGG intervention, both low and high LGG doses were shown to improve the observed OTU, Chao1, ACE, and Shannon indices, while the Simpson index decreased, demonstrating that LGG can promote intestinal microbiota abundance and diversity. Furthermore, LGG treatment increased the abundances of intestinal Firmicutes, Bacteroides and Actinomycetes while reducing that of Proteobacteria. In addition to its effect on gut the microbiota, LGG could also regulate the host immune system. In the present study, we showed that LGG could affect the percentage of CD3+ T lymphocytes in the spleens (SPLs), mesenteric lymph nodes (MLNs), Peyer’s patches (PPs) and lamina propria lymphocytes (LPLs) of mice, including total CD3+ T, CD3+CD4+ T, and CD3+CD8+ T lymphocytes. Furthermore, LGG could effectively increase the expression of Th1-type cytokines (IFN-γ) and Th2 cytokines (IL-4) in CD4+ T cells, indicating that the proportion of Th1 and Th2 cells in mice with LGG treatment was in a high equilibrium state compared to the control group. In addition, the IFN-γ/IL-4 ratio was greater than 1 in mice with LGG intervention, suggesting that LGG tends to mediate the Th1 immune response. The results of the present study also showed that LGG upregulated the expression of IL-17 in CD4+ T cells and regulated the percentage of CD4+CD25+Foxp3+ Treg cells in various secondary immunological organs, indicating that LGG may promote the balance of Th-17 and Treg cells. |
| first_indexed | 2024-12-23T04:23:02Z |
| format | Article |
| id | doaj.art-a2404e670b1949d7bd6232937af0db13 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T04:23:02Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a2404e670b1949d7bd6232937af0db132022-12-21T18:00:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-12-011110.3389/fmicb.2020.607735607735Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell DifferentiationChun-wei Shi0Chun-wei Shi1Chun-wei Shi2Ming-yang Cheng3Ming-yang Cheng4Ming-yang Cheng5Xin Yang6Xin Yang7Xin Yang8Yi-yuan Lu9Yi-yuan Lu10Yi-yuan Lu11Hong-duo Yin12Hong-duo Yin13Hong-duo Yin14Yan Zeng15Yan Zeng16Yan Zeng17Ru-yu Wang18Ru-yu Wang19Ru-yu Wang20Yan-long Jiang21Yan-long Jiang22Yan-long Jiang23Wen-tao Yang24Wen-tao Yang25Wen-tao Yang26Jian-zhong Wang27Jian-zhong Wang28Jian-zhong Wang29Dan-dan Zhao30Dan-dan Zhao31Dan-dan Zhao32Hai-bin Huang33Hai-bin Huang34Hai-bin Huang35Li-ping Ye36Li-ping Ye37Li-ping Ye38Xin Cao39Xin Cao40Xin Cao41Gui-lian Yang42Gui-lian Yang43Gui-lian Yang44Chun-feng Wang45Chun-feng Wang46Chun-feng Wang47College of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaJilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, ChinaLactic acid bacteria (LAB) are the primary genera of the intestinal flora and have many probiotic functions. In the present study, Lactobacillus rhamnosus GG (LGG) ATCC 53103 was used to treat BALB/c mice. After LGG intervention, both low and high LGG doses were shown to improve the observed OTU, Chao1, ACE, and Shannon indices, while the Simpson index decreased, demonstrating that LGG can promote intestinal microbiota abundance and diversity. Furthermore, LGG treatment increased the abundances of intestinal Firmicutes, Bacteroides and Actinomycetes while reducing that of Proteobacteria. In addition to its effect on gut the microbiota, LGG could also regulate the host immune system. In the present study, we showed that LGG could affect the percentage of CD3+ T lymphocytes in the spleens (SPLs), mesenteric lymph nodes (MLNs), Peyer’s patches (PPs) and lamina propria lymphocytes (LPLs) of mice, including total CD3+ T, CD3+CD4+ T, and CD3+CD8+ T lymphocytes. Furthermore, LGG could effectively increase the expression of Th1-type cytokines (IFN-γ) and Th2 cytokines (IL-4) in CD4+ T cells, indicating that the proportion of Th1 and Th2 cells in mice with LGG treatment was in a high equilibrium state compared to the control group. In addition, the IFN-γ/IL-4 ratio was greater than 1 in mice with LGG intervention, suggesting that LGG tends to mediate the Th1 immune response. The results of the present study also showed that LGG upregulated the expression of IL-17 in CD4+ T cells and regulated the percentage of CD4+CD25+Foxp3+ Treg cells in various secondary immunological organs, indicating that LGG may promote the balance of Th-17 and Treg cells.https://www.frontiersin.org/articles/10.3389/fmicb.2020.607735/fullLactobacillus rhamnosusgut microbiotaT lymphocytesprobioticmucosal immunity |
| spellingShingle | Chun-wei Shi Chun-wei Shi Chun-wei Shi Ming-yang Cheng Ming-yang Cheng Ming-yang Cheng Xin Yang Xin Yang Xin Yang Yi-yuan Lu Yi-yuan Lu Yi-yuan Lu Hong-duo Yin Hong-duo Yin Hong-duo Yin Yan Zeng Yan Zeng Yan Zeng Ru-yu Wang Ru-yu Wang Ru-yu Wang Yan-long Jiang Yan-long Jiang Yan-long Jiang Wen-tao Yang Wen-tao Yang Wen-tao Yang Jian-zhong Wang Jian-zhong Wang Jian-zhong Wang Dan-dan Zhao Dan-dan Zhao Dan-dan Zhao Hai-bin Huang Hai-bin Huang Hai-bin Huang Li-ping Ye Li-ping Ye Li-ping Ye Xin Cao Xin Cao Xin Cao Gui-lian Yang Gui-lian Yang Gui-lian Yang Chun-feng Wang Chun-feng Wang Chun-feng Wang Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation Frontiers in Microbiology Lactobacillus rhamnosus gut microbiota T lymphocytes probiotic mucosal immunity |
| title | Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation |
| title_full | Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation |
| title_fullStr | Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation |
| title_full_unstemmed | Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation |
| title_short | Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation |
| title_sort | probiotic lactobacillus rhamnosus gg promotes mouse gut microbiota diversity and t cell differentiation |
| topic | Lactobacillus rhamnosus gut microbiota T lymphocytes probiotic mucosal immunity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.607735/full |
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