Physicochemical and Antioxidant Properties of Gelatin and Gelatin Hydrolysates Obtained from Extrusion-Pretreated Fish (<i>Oreochromis</i> sp.) Scales
Fish gelatin and its hydrolysates exhibit a variety of biological characteristics, which include antihypertensive and antioxidant properties. In this study, fish gelatins were extracted from extrusion-pretreated tilapia scales, and then subjected to analyses to determine the physicochemical properti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/5/275 |
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| author | Wei-Cheng Shiao Tien-Chiu Wu Chia-Hung Kuo Yung-Hsiang Tsai Mei-Ling Tsai Yong-Han Hong Chun-Yung Huang |
| author_facet | Wei-Cheng Shiao Tien-Chiu Wu Chia-Hung Kuo Yung-Hsiang Tsai Mei-Ling Tsai Yong-Han Hong Chun-Yung Huang |
| author_sort | Wei-Cheng Shiao |
| collection | DOAJ |
| description | Fish gelatin and its hydrolysates exhibit a variety of biological characteristics, which include antihypertensive and antioxidant properties. In this study, fish gelatins were extracted from extrusion-pretreated tilapia scales, and then subjected to analyses to determine the physicochemical properties and antioxidant activity of the extracted gelatins. Our findings indicate that TSG2 (preconditioned with 1.26% citric acid) possessed the greatest extraction yield, as well as higher antioxidant activities compared with the other extracted gelatins. Hence, TSG2 was subjected to further hydrolyzation using different proteases and ultrafiltration conditions, which yielded four gelatin hydrolysates: TSGH1, TSGH2, TSGH3, and TSGH4. The results showed that TSGH4 (Pepsin + Pancreatin and ultrafiltration < 3000 Da) had a higher yield and greater antioxidant activity in comparison with the other gelatin hydrolysates. As such, TSGH4 was subjected to further fractionation using a Superdex peptide column and two-stage reverse-phase column HPLC chromatography, yielding a subfraction TSGH4-6-2-b, which possessed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity compared with the other fractions. Further LC-ESI/MS/MS analysis of TSGH4-6-2-b suggested two novel peptides (GYDEY and EPGKSGEQGAPGEAGAP), which could have potential as naturally-occurring peptides with antioxidant properties. These promising results suggest that these antioxidant peptides could have applications in food products, nutraceuticals, and cosmetics. |
| first_indexed | 2024-03-10T11:25:04Z |
| format | Article |
| id | doaj.art-a2429ae4f02d4d78bc9e69916b1b3079 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T11:25:04Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a2429ae4f02d4d78bc9e69916b1b30792023-11-21T19:43:37ZengMDPI AGMarine Drugs1660-33972021-05-0119527510.3390/md19050275Physicochemical and Antioxidant Properties of Gelatin and Gelatin Hydrolysates Obtained from Extrusion-Pretreated Fish (<i>Oreochromis</i> sp.) ScalesWei-Cheng Shiao0Tien-Chiu Wu1Chia-Hung Kuo2Yung-Hsiang Tsai3Mei-Ling Tsai4Yong-Han Hong5Chun-Yung Huang6Division of Gastroenterology (General Medicine), Department of Internal Medicine, Yuan’s General Hospital, No.162, Cheng Kung 1st Rd., Lingya District, Kaohsiung 80249, TaiwanDivision of Hematology and Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung 81157, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung 81157, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung 81157, TaiwanDepartment of Nutrition, I-Shou University (Yanchao Campus), No.8, Yida Rd., Jiaosu Village, Yanchao District, Kaohsiung 82445, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung 81157, TaiwanFish gelatin and its hydrolysates exhibit a variety of biological characteristics, which include antihypertensive and antioxidant properties. In this study, fish gelatins were extracted from extrusion-pretreated tilapia scales, and then subjected to analyses to determine the physicochemical properties and antioxidant activity of the extracted gelatins. Our findings indicate that TSG2 (preconditioned with 1.26% citric acid) possessed the greatest extraction yield, as well as higher antioxidant activities compared with the other extracted gelatins. Hence, TSG2 was subjected to further hydrolyzation using different proteases and ultrafiltration conditions, which yielded four gelatin hydrolysates: TSGH1, TSGH2, TSGH3, and TSGH4. The results showed that TSGH4 (Pepsin + Pancreatin and ultrafiltration < 3000 Da) had a higher yield and greater antioxidant activity in comparison with the other gelatin hydrolysates. As such, TSGH4 was subjected to further fractionation using a Superdex peptide column and two-stage reverse-phase column HPLC chromatography, yielding a subfraction TSGH4-6-2-b, which possessed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity compared with the other fractions. Further LC-ESI/MS/MS analysis of TSGH4-6-2-b suggested two novel peptides (GYDEY and EPGKSGEQGAPGEAGAP), which could have potential as naturally-occurring peptides with antioxidant properties. These promising results suggest that these antioxidant peptides could have applications in food products, nutraceuticals, and cosmetics.https://www.mdpi.com/1660-3397/19/5/275antioxidantenzyme digestionextrusionfish scalesgelatingelatin hydrolysate |
| spellingShingle | Wei-Cheng Shiao Tien-Chiu Wu Chia-Hung Kuo Yung-Hsiang Tsai Mei-Ling Tsai Yong-Han Hong Chun-Yung Huang Physicochemical and Antioxidant Properties of Gelatin and Gelatin Hydrolysates Obtained from Extrusion-Pretreated Fish (<i>Oreochromis</i> sp.) Scales Marine Drugs antioxidant enzyme digestion extrusion fish scales gelatin gelatin hydrolysate |
| title | Physicochemical and Antioxidant Properties of Gelatin and Gelatin Hydrolysates Obtained from Extrusion-Pretreated Fish (<i>Oreochromis</i> sp.) Scales |
| title_full | Physicochemical and Antioxidant Properties of Gelatin and Gelatin Hydrolysates Obtained from Extrusion-Pretreated Fish (<i>Oreochromis</i> sp.) Scales |
| title_fullStr | Physicochemical and Antioxidant Properties of Gelatin and Gelatin Hydrolysates Obtained from Extrusion-Pretreated Fish (<i>Oreochromis</i> sp.) Scales |
| title_full_unstemmed | Physicochemical and Antioxidant Properties of Gelatin and Gelatin Hydrolysates Obtained from Extrusion-Pretreated Fish (<i>Oreochromis</i> sp.) Scales |
| title_short | Physicochemical and Antioxidant Properties of Gelatin and Gelatin Hydrolysates Obtained from Extrusion-Pretreated Fish (<i>Oreochromis</i> sp.) Scales |
| title_sort | physicochemical and antioxidant properties of gelatin and gelatin hydrolysates obtained from extrusion pretreated fish i oreochromis i sp scales |
| topic | antioxidant enzyme digestion extrusion fish scales gelatin gelatin hydrolysate |
| url | https://www.mdpi.com/1660-3397/19/5/275 |
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