| Summary: | A comparison between the classical gas chromatography (GC) technique with supercritical fluid chromatography (SFC) technology was performed using an essential oil of <i>Citrus limon</i> (lemon) as a model, considering its wide use in the cosmetic world. For the qualitative part, the signal annotation was achieved by mass spectrometry using either an electron ionization (EI) or an atmospheric pressure photoionization (APPI) source. For the quantitative part, GC was hyphenated to a flame ionization detector (GC-FID) and SFC to an ultraviolet detector (SFC-UV). The assay of the major component of citrus oil, i.e., limonene, was carried out by SFC-UV. The similar results obtained between GC-FID and SFC-UV allows SFC-UV to be considered as an alternative to GC-FID for <i>Citrus</i> oil characterization. Then, analyses of an essential oil collection from <i>Citrus</i> fruits were achieved to confirm the potential use of SFC-UV for oil classification in the context of quality control of raw materials in cosmetics.
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