Callus induction of three turf grass and analysis of their proteins after dehydration treatment(三种草坪草愈伤组织的诱导及其脱水处理后的蛋白分析)
研究了匍匐翦股颖、高羊茅和狗牙根3种草坪草愈伤组织诱导情况，愈伤组织诱导率分别为53.0 ± 8.7%、60.7±7.9%和14.7±4.0%，三者的愈伤组织含水量约为91.4 ± 0.3%、94.1 ± 0.4%和93.4 ± 0.2%.经空气干燥法脱水处理，两种冷季型草坪草匍匐翦股颖和高羊茅愈伤组织中18.5 kDa和28.7 kDa的热稳定蛋白表达量增加.暖季型草坪草狗牙根中18.5 kDa的热稳定蛋白表达量增加，而35 kDa的热稳定蛋白表达量明显减少.经脱水蛋白的Western Blot检测，脱水处理后3种草坪草中均有脱水蛋白大量积累，分子量从18.5到34.3 kDa不等.暖季型和...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2008-07-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2008.04.021 |
| _version_ | 1797236168143667200 |
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| author | WUQiong(吴琼) ZHANGKe-han(张可菡) XUXiao-hong(徐小红) CHENZhe-hao(陈哲皓) BIANHong-wu(边红武) |
| author_facet | WUQiong(吴琼) ZHANGKe-han(张可菡) XUXiao-hong(徐小红) CHENZhe-hao(陈哲皓) BIANHong-wu(边红武) |
| author_sort | WUQiong(吴琼) |
| collection | DOAJ |
| description | 研究了匍匐翦股颖、高羊茅和狗牙根3种草坪草愈伤组织诱导情况，愈伤组织诱导率分别为53.0 ± 8.7%、60.7±7.9%和14.7±4.0%，三者的愈伤组织含水量约为91.4 ± 0.3%、94.1 ± 0.4%和93.4 ± 0.2%.经空气干燥法脱水处理，两种冷季型草坪草匍匐翦股颖和高羊茅愈伤组织中18.5 kDa和28.7 kDa的热稳定蛋白表达量增加.暖季型草坪草狗牙根中18.5 kDa的热稳定蛋白表达量增加，而35 kDa的热稳定蛋白表达量明显减少.经脱水蛋白的Western Blot检测，脱水处理后3种草坪草中均有脱水蛋白大量积累，分子量从18.5到34.3 kDa不等.暖季型和冷季型草坪草在热稳定蛋白和脱水蛋白表达上存在的差异，可能与它们的耐旱机理及耐旱性强弱差异有关. |
| first_indexed | 2024-04-24T16:59:34Z |
| format | Article |
| id | doaj.art-a247a66542504102a915aecfd18ed64f |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T16:59:34Z |
| publishDate | 2008-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-a247a66542504102a915aecfd18ed64f2024-03-29T01:58:26ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972008-07-0135445545910.3785/j.issn.1008-9497.2008.04.021Callus induction of three turf grass and analysis of their proteins after dehydration treatment(三种草坪草愈伤组织的诱导及其脱水处理后的蛋白分析)WUQiong(吴琼)0ZHANGKe-han(张可菡)1XUXiao-hong(徐小红)2CHENZhe-hao(陈哲皓)3BIANHong-wu(边红武)4 1.College of Life Sciences, Zhejiang University, Hangzhou 310058, China( 1.浙江大学生命科学学院，浙江 杭州 310058) 2.College of Management, Shanghai University of Engineering Science, Shanghai 201620, China( 2.上海工程技术大学管理学院，上海 201620) 1.College of Life Sciences, Zhejiang University, Hangzhou 310058, China( 1.浙江大学生命科学学院，浙江 杭州 310058) 1.College of Life Sciences, Zhejiang University, Hangzhou 310058, China( 1.浙江大学生命科学学院，浙江 杭州 310058) 1.College of Life Sciences, Zhejiang University, Hangzhou 310058, China( 1.浙江大学生命科学学院，浙江 杭州 310058)研究了匍匐翦股颖、高羊茅和狗牙根3种草坪草愈伤组织诱导情况，愈伤组织诱导率分别为53.0 ± 8.7%、60.7±7.9%和14.7±4.0%，三者的愈伤组织含水量约为91.4 ± 0.3%、94.1 ± 0.4%和93.4 ± 0.2%.经空气干燥法脱水处理，两种冷季型草坪草匍匐翦股颖和高羊茅愈伤组织中18.5 kDa和28.7 kDa的热稳定蛋白表达量增加.暖季型草坪草狗牙根中18.5 kDa的热稳定蛋白表达量增加，而35 kDa的热稳定蛋白表达量明显减少.经脱水蛋白的Western Blot检测，脱水处理后3种草坪草中均有脱水蛋白大量积累，分子量从18.5到34.3 kDa不等.暖季型和冷季型草坪草在热稳定蛋白和脱水蛋白表达上存在的差异，可能与它们的耐旱机理及耐旱性强弱差异有关.https://doi.org/10.3785/j.issn.1008-9497.2008.04.021草坪草愈伤组织脱水处理热稳定蛋白脱水蛋白 |
| spellingShingle | WUQiong(吴琼) ZHANGKe-han(张可菡) XUXiao-hong(徐小红) CHENZhe-hao(陈哲皓) BIANHong-wu(边红武) Callus induction of three turf grass and analysis of their proteins after dehydration treatment(三种草坪草愈伤组织的诱导及其脱水处理后的蛋白分析) Zhejiang Daxue xuebao. Lixue ban 草坪草 愈伤组织 脱水处理 热稳定蛋白 脱水蛋白 |
| title | Callus induction of three turf grass and analysis of their proteins after dehydration treatment(三种草坪草愈伤组织的诱导及其脱水处理后的蛋白分析) |
| title_full | Callus induction of three turf grass and analysis of their proteins after dehydration treatment(三种草坪草愈伤组织的诱导及其脱水处理后的蛋白分析) |
| title_fullStr | Callus induction of three turf grass and analysis of their proteins after dehydration treatment(三种草坪草愈伤组织的诱导及其脱水处理后的蛋白分析) |
| title_full_unstemmed | Callus induction of three turf grass and analysis of their proteins after dehydration treatment(三种草坪草愈伤组织的诱导及其脱水处理后的蛋白分析) |
| title_short | Callus induction of three turf grass and analysis of their proteins after dehydration treatment(三种草坪草愈伤组织的诱导及其脱水处理后的蛋白分析) |
| title_sort | callus induction of three turf grass and analysis of their proteins after dehydration treatment 三种草坪草愈伤组织的诱导及其脱水处理后的蛋白分析 |
| topic | 草坪草 愈伤组织 脱水处理 热稳定蛋白 脱水蛋白 |
| url | https://doi.org/10.3785/j.issn.1008-9497.2008.04.021 |
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