Palmitic acid-modified GnRH-Th epitope peptide immunocastration vaccine (W/O/W adjuvant) can effectively ensure the castration and reduce the smelly smell in boars
IntroductionRecent studies have demonstrated the effectiveness of Gonadotropin-releasing hormone (GnRH) in inhibiting testicular growth and development in male animals to achieve castration while improving the meat quality of various livestock species, including cattle, sheep, goats, and pigs.Method...
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1174770/full |
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| author | Huayi Gao Huayi Gao Kun Liu Lu Zhang Yongfang Wang Xubin Fu Xubin Fu Yujie Guo Mei Bai Yanbing Shen Min Wang |
| author_facet | Huayi Gao Huayi Gao Kun Liu Lu Zhang Yongfang Wang Xubin Fu Xubin Fu Yujie Guo Mei Bai Yanbing Shen Min Wang |
| author_sort | Huayi Gao |
| collection | DOAJ |
| description | IntroductionRecent studies have demonstrated the effectiveness of Gonadotropin-releasing hormone (GnRH) in inhibiting testicular growth and development in male animals to achieve castration while improving the meat quality of various livestock species, including cattle, sheep, goats, and pigs.MethodsIn this research, a GnRH-Th vaccine was synthesized using the Fmoc solid-phase synthesis technique, and the T helper (Th) antigen was modified with palmitic acid to improve its efficacy. The vaccine was then coated with a water-in-oil-in-water adjuvant to improve stability and safety. After passing safety and stability tests, the vaccine was administered to 13-week-old boars.ResultsThe results showed that it was stable, safe, and effective for up to 15 months. Moreover, the vaccine did not negatively affect the growth rate and body weight of the pigs. The palmitic acid-modified “GnRH-Th epitope peptide immunocastration vaccine (Water-in-Oil-in-Water (W/O/W)) effectively reduced the testosterone concentration and achieved castration. The concentration of androstenone and skatole hormones significantly decreased, leading to improved meat quality in the boars. The boars were then slaughtered at 33 weeks of age, and the results showed that the meat quality of the vaccinated boars was superior to that of the non-vaccinated control group (p < 0.05).DiscussionThis study demonstrated that GnRH can safely and effectively achieve immune castration in boars after coupling T cell epitopes, palmitic acid modification and W-O-W coating. Provide a better method for the further development of GnRH and the realization of animal welfare. |
| first_indexed | 2024-04-09T16:17:16Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-09T16:17:16Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-a247ebba544542f29e0e3cf258df4d102023-04-24T04:21:22ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-04-011010.3389/fvets.2023.11747701174770Palmitic acid-modified GnRH-Th epitope peptide immunocastration vaccine (W/O/W adjuvant) can effectively ensure the castration and reduce the smelly smell in boarsHuayi Gao0Huayi Gao1Kun Liu2Lu Zhang3Yongfang Wang4Xubin Fu5Xubin Fu6Yujie Guo7Mei Bai8Yanbing Shen9Min Wang10Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaTianjin Ringpu Bio-technology Co., Ltd., Tianjin, ChinaDr. Bing Zhang Department of Statistics, College of Arts and Sciences, University of Kentucky, Lexington, KY, United StatesTianjin Ringpu Bio-technology Co., Ltd., Tianjin, ChinaTianjin Customs Animal and Plant and Foodstuffs Inspection Center, Tianjin, ChinaTianjin Ringpu Bio-technology Co., Ltd., Tianjin, ChinaTianjin Agricultural University, Tianjin, ChinaTianjin Ringpu Bio-technology Co., Ltd., Tianjin, ChinaTianjin Agricultural University, Tianjin, ChinaKey Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaKey Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaIntroductionRecent studies have demonstrated the effectiveness of Gonadotropin-releasing hormone (GnRH) in inhibiting testicular growth and development in male animals to achieve castration while improving the meat quality of various livestock species, including cattle, sheep, goats, and pigs.MethodsIn this research, a GnRH-Th vaccine was synthesized using the Fmoc solid-phase synthesis technique, and the T helper (Th) antigen was modified with palmitic acid to improve its efficacy. The vaccine was then coated with a water-in-oil-in-water adjuvant to improve stability and safety. After passing safety and stability tests, the vaccine was administered to 13-week-old boars.ResultsThe results showed that it was stable, safe, and effective for up to 15 months. Moreover, the vaccine did not negatively affect the growth rate and body weight of the pigs. The palmitic acid-modified “GnRH-Th epitope peptide immunocastration vaccine (Water-in-Oil-in-Water (W/O/W)) effectively reduced the testosterone concentration and achieved castration. The concentration of androstenone and skatole hormones significantly decreased, leading to improved meat quality in the boars. The boars were then slaughtered at 33 weeks of age, and the results showed that the meat quality of the vaccinated boars was superior to that of the non-vaccinated control group (p < 0.05).DiscussionThis study demonstrated that GnRH can safely and effectively achieve immune castration in boars after coupling T cell epitopes, palmitic acid modification and W-O-W coating. Provide a better method for the further development of GnRH and the realization of animal welfare.https://www.frontiersin.org/articles/10.3389/fvets.2023.1174770/fullimmune castration vaccinegonadotropin release hormonereduce the smelly smellgonadotropin-releasing hormoneboar castration |
| spellingShingle | Huayi Gao Huayi Gao Kun Liu Lu Zhang Yongfang Wang Xubin Fu Xubin Fu Yujie Guo Mei Bai Yanbing Shen Min Wang Palmitic acid-modified GnRH-Th epitope peptide immunocastration vaccine (W/O/W adjuvant) can effectively ensure the castration and reduce the smelly smell in boars Frontiers in Veterinary Science immune castration vaccine gonadotropin release hormone reduce the smelly smell gonadotropin-releasing hormone boar castration |
| title | Palmitic acid-modified GnRH-Th epitope peptide immunocastration vaccine (W/O/W adjuvant) can effectively ensure the castration and reduce the smelly smell in boars |
| title_full | Palmitic acid-modified GnRH-Th epitope peptide immunocastration vaccine (W/O/W adjuvant) can effectively ensure the castration and reduce the smelly smell in boars |
| title_fullStr | Palmitic acid-modified GnRH-Th epitope peptide immunocastration vaccine (W/O/W adjuvant) can effectively ensure the castration and reduce the smelly smell in boars |
| title_full_unstemmed | Palmitic acid-modified GnRH-Th epitope peptide immunocastration vaccine (W/O/W adjuvant) can effectively ensure the castration and reduce the smelly smell in boars |
| title_short | Palmitic acid-modified GnRH-Th epitope peptide immunocastration vaccine (W/O/W adjuvant) can effectively ensure the castration and reduce the smelly smell in boars |
| title_sort | palmitic acid modified gnrh th epitope peptide immunocastration vaccine w o w adjuvant can effectively ensure the castration and reduce the smelly smell in boars |
| topic | immune castration vaccine gonadotropin release hormone reduce the smelly smell gonadotropin-releasing hormone boar castration |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1174770/full |
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