Kinetic study on the reaction of sodium nitrite with neurotransmitters secreted in the stomach
Abstract Nitroso-compounds are potentially mutagenic and carcinogenic compounds due to their ability to alkylate DNA bases. One of the most common sources of human exposure to nitroso-compounds is their formation in the acidic environment of the stomach by the reaction between electron-rich molecule...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-42759-x |
| _version_ | 1797559989328412672 |
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| author | Mario González-Jiménez M. Pilar García-Santos Blanca Bermejo Tesón Ángel L. Fuentes de Arriba Jorge Arenas Valgañón Emilio Calle Julio Casado |
| author_facet | Mario González-Jiménez M. Pilar García-Santos Blanca Bermejo Tesón Ángel L. Fuentes de Arriba Jorge Arenas Valgañón Emilio Calle Julio Casado |
| author_sort | Mario González-Jiménez |
| collection | DOAJ |
| description | Abstract Nitroso-compounds are potentially mutagenic and carcinogenic compounds due to their ability to alkylate DNA bases. One of the most common sources of human exposure to nitroso-compounds is their formation in the acidic environment of the stomach by the reaction between electron-rich molecules present in the lumen and sodium nitrite ingested in the diet. To date, the formation of nitroso-compounds by the reaction of nitrite with food components has been investigated in depth, but little attention has been paid to substances secreted in the stomach, such as dopamine or serotonin, whose reaction products with nitrite have proven mutagenic properties. In this article, we present a kinetic study with UV–visible spectroscopy of the nitrosation reactions of both molecules, as well as of L-tyrosine, the amino-acid precursor of dopamine. We determined the kinetic parameters and reaction mechanisms for the reactions, studying the influence of the reactants concentration, pH, temperature, and ionic strength on the reaction rate. In all cases, the favoured reaction product was a stable nitroso-compound. Serotonin, the molecule whose product was the most mutagenic, underwent two consecutive nitrosation reactions. These findings suggest that additional biological research is needed to understand how this reaction alters the function of these neurotransmitters as well as the potentially toxic effects they may have once nitrosated. |
| first_indexed | 2024-03-10T17:54:02Z |
| format | Article |
| id | doaj.art-a249902a54d443498c26b3e2b4abdff7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:54:02Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a249902a54d443498c26b3e2b4abdff72023-11-20T09:16:26ZengNature PortfolioScientific Reports2045-23222023-09-0113111110.1038/s41598-023-42759-xKinetic study on the reaction of sodium nitrite with neurotransmitters secreted in the stomachMario González-Jiménez0M. Pilar García-Santos1Blanca Bermejo Tesón2Ángel L. Fuentes de Arriba3Jorge Arenas Valgañón4Emilio Calle5Julio Casado6Departamento de Química Física, Universidad de SalamancaDepartamento de Química Física, Universidad de SalamancaDepartamento de Química Física, Universidad de SalamancaDepartamento de Química Orgánica, Universidad de SalamancaDepartamento de Química Física, Universidad de SalamancaDepartamento de Química Física, Universidad de SalamancaDepartamento de Química Física, Universidad de SalamancaAbstract Nitroso-compounds are potentially mutagenic and carcinogenic compounds due to their ability to alkylate DNA bases. One of the most common sources of human exposure to nitroso-compounds is their formation in the acidic environment of the stomach by the reaction between electron-rich molecules present in the lumen and sodium nitrite ingested in the diet. To date, the formation of nitroso-compounds by the reaction of nitrite with food components has been investigated in depth, but little attention has been paid to substances secreted in the stomach, such as dopamine or serotonin, whose reaction products with nitrite have proven mutagenic properties. In this article, we present a kinetic study with UV–visible spectroscopy of the nitrosation reactions of both molecules, as well as of L-tyrosine, the amino-acid precursor of dopamine. We determined the kinetic parameters and reaction mechanisms for the reactions, studying the influence of the reactants concentration, pH, temperature, and ionic strength on the reaction rate. In all cases, the favoured reaction product was a stable nitroso-compound. Serotonin, the molecule whose product was the most mutagenic, underwent two consecutive nitrosation reactions. These findings suggest that additional biological research is needed to understand how this reaction alters the function of these neurotransmitters as well as the potentially toxic effects they may have once nitrosated.https://doi.org/10.1038/s41598-023-42759-x |
| spellingShingle | Mario González-Jiménez M. Pilar García-Santos Blanca Bermejo Tesón Ángel L. Fuentes de Arriba Jorge Arenas Valgañón Emilio Calle Julio Casado Kinetic study on the reaction of sodium nitrite with neurotransmitters secreted in the stomach Scientific Reports |
| title | Kinetic study on the reaction of sodium nitrite with neurotransmitters secreted in the stomach |
| title_full | Kinetic study on the reaction of sodium nitrite with neurotransmitters secreted in the stomach |
| title_fullStr | Kinetic study on the reaction of sodium nitrite with neurotransmitters secreted in the stomach |
| title_full_unstemmed | Kinetic study on the reaction of sodium nitrite with neurotransmitters secreted in the stomach |
| title_short | Kinetic study on the reaction of sodium nitrite with neurotransmitters secreted in the stomach |
| title_sort | kinetic study on the reaction of sodium nitrite with neurotransmitters secreted in the stomach |
| url | https://doi.org/10.1038/s41598-023-42759-x |
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