Cytotoxicity of two different intercanal medicaments on human gingival fibroblasts - A Laboratory study
Aim: Intracanal medicaments are often recommended during endodontic sessions to eliminate the necrotic debris and microorganisms. The aim of the present study was to observe the cytotoxicity of calcium hydroxide (CH) and colchicine (COL), at different concentrations, on human gingival fibroblast cel...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2022-01-01
|
| Series: | Endodontology |
| Subjects: | |
| Online Access: | http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2022;volume=34;issue=2;spage=76;epage=79;aulast=Barakatein |
| _version_ | 1811294517527052288 |
|---|---|
| author | Behnaz Barakatein Alireza Farhad Elham Shadmehr Hamidreza Mohammad Sharifi Masoud Mohammadi Hamidreza Mohamad Sharif Amin Davoudi |
| author_facet | Behnaz Barakatein Alireza Farhad Elham Shadmehr Hamidreza Mohammad Sharifi Masoud Mohammadi Hamidreza Mohamad Sharif Amin Davoudi |
| author_sort | Behnaz Barakatein |
| collection | DOAJ |
| description | Aim: Intracanal medicaments are often recommended during endodontic sessions to eliminate the necrotic debris and microorganisms. The aim of the present study was to observe the cytotoxicity of calcium hydroxide (CH) and colchicine (COL), at different concentrations, on human gingival fibroblast cells.
Materials and Methods: Human gingival fibroblasts were cultured on plastic flasks containing RPMI 1640 media and fetal calf serum 10% supplemented with antibiotic agents. Trypsin/ethylenediaminetetraacetic acid 0.2% enzyme was used to isolate the cells, and the suspension was transferred to tubes for centrifuging. Conventional CH and COL were separately mixed with sterile saline solution to prepare a stock media. By serial dilution of stock media, desired concentrations were prepared at 2, 1.75, 1.5, and 1.25 mg/ml, separately. After considering a control group, the cells were exposed to test materials. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted at 24 h, 72 h, and 7 days later. Optical density (OD) was evaluated to attain cell viability percentage. Finally, the recorded data were analyzed by Kruskal–Wallis and Mann–Whitney tests using SPSS software version 19 at a significant level of 0.05.
Results: The highest (1.40 ± 0.66) and lowest (0.15 ± 0.00) ODs were observed in CH 1.25 mg/ml and COL 1.5 mg/ml after 72 h, respectively. All of the concentrations of both CH and COL showed significant OD differences with the control group (all P = 0.001).
Conclusion: Both CH and COL manifested similar cytotoxicity on human gingival fibroblast cells. |
| first_indexed | 2024-04-13T05:17:38Z |
| format | Article |
| id | doaj.art-a24a31f8e3104628976a1a493424a9ac |
| institution | Directory Open Access Journal |
| issn | 0970-7212 |
| language | English |
| last_indexed | 2024-04-13T05:17:38Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Endodontology |
| spelling | doaj.art-a24a31f8e3104628976a1a493424a9ac2022-12-22T03:00:50ZengWolters Kluwer Medknow PublicationsEndodontology0970-72122022-01-01342767910.4103/endo.endo_107_20Cytotoxicity of two different intercanal medicaments on human gingival fibroblasts - A Laboratory studyBehnaz BarakateinAlireza FarhadElham ShadmehrHamidreza Mohammad SharifiMasoud Mohammadi Hamidreza Mohamad SharifAmin DavoudiAim: Intracanal medicaments are often recommended during endodontic sessions to eliminate the necrotic debris and microorganisms. The aim of the present study was to observe the cytotoxicity of calcium hydroxide (CH) and colchicine (COL), at different concentrations, on human gingival fibroblast cells. Materials and Methods: Human gingival fibroblasts were cultured on plastic flasks containing RPMI 1640 media and fetal calf serum 10% supplemented with antibiotic agents. Trypsin/ethylenediaminetetraacetic acid 0.2% enzyme was used to isolate the cells, and the suspension was transferred to tubes for centrifuging. Conventional CH and COL were separately mixed with sterile saline solution to prepare a stock media. By serial dilution of stock media, desired concentrations were prepared at 2, 1.75, 1.5, and 1.25 mg/ml, separately. After considering a control group, the cells were exposed to test materials. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted at 24 h, 72 h, and 7 days later. Optical density (OD) was evaluated to attain cell viability percentage. Finally, the recorded data were analyzed by Kruskal–Wallis and Mann–Whitney tests using SPSS software version 19 at a significant level of 0.05. Results: The highest (1.40 ± 0.66) and lowest (0.15 ± 0.00) ODs were observed in CH 1.25 mg/ml and COL 1.5 mg/ml after 72 h, respectively. All of the concentrations of both CH and COL showed significant OD differences with the control group (all P = 0.001). Conclusion: Both CH and COL manifested similar cytotoxicity on human gingival fibroblast cells.http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2022;volume=34;issue=2;spage=76;epage=79;aulast=Barakateincalcium hydroxidecolchicinecytotoxicity testfibroblast |
| spellingShingle | Behnaz Barakatein Alireza Farhad Elham Shadmehr Hamidreza Mohammad Sharifi Masoud Mohammadi Hamidreza Mohamad Sharif Amin Davoudi Cytotoxicity of two different intercanal medicaments on human gingival fibroblasts - A Laboratory study Endodontology calcium hydroxide colchicine cytotoxicity test fibroblast |
| title | Cytotoxicity of two different intercanal medicaments on human gingival fibroblasts - A Laboratory study |
| title_full | Cytotoxicity of two different intercanal medicaments on human gingival fibroblasts - A Laboratory study |
| title_fullStr | Cytotoxicity of two different intercanal medicaments on human gingival fibroblasts - A Laboratory study |
| title_full_unstemmed | Cytotoxicity of two different intercanal medicaments on human gingival fibroblasts - A Laboratory study |
| title_short | Cytotoxicity of two different intercanal medicaments on human gingival fibroblasts - A Laboratory study |
| title_sort | cytotoxicity of two different intercanal medicaments on human gingival fibroblasts a laboratory study |
| topic | calcium hydroxide colchicine cytotoxicity test fibroblast |
| url | http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2022;volume=34;issue=2;spage=76;epage=79;aulast=Barakatein |
| work_keys_str_mv | AT behnazbarakatein cytotoxicityoftwodifferentintercanalmedicamentsonhumangingivalfibroblastsalaboratorystudy AT alirezafarhad cytotoxicityoftwodifferentintercanalmedicamentsonhumangingivalfibroblastsalaboratorystudy AT elhamshadmehr cytotoxicityoftwodifferentintercanalmedicamentsonhumangingivalfibroblastsalaboratorystudy AT hamidrezamohammadsharifi cytotoxicityoftwodifferentintercanalmedicamentsonhumangingivalfibroblastsalaboratorystudy AT masoudmohammadihamidrezamohamadsharif cytotoxicityoftwodifferentintercanalmedicamentsonhumangingivalfibroblastsalaboratorystudy AT amindavoudi cytotoxicityoftwodifferentintercanalmedicamentsonhumangingivalfibroblastsalaboratorystudy |