<i>Arabidopsis</i> CCoAOMT1 Plays a Role in Drought Stress Response via ROS- and ABA-Dependent Manners
Plants possess adaptive reprogramed modules to prolonged environmental stresses, including adjustment of metabolism and gene expression for physiological and morphological adaptation. <i>CCoAOMT1</i> encodes a caffeoyl CoA O-methyltransferase and is known to play an important role in ada...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/5/831 |
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| author | Hyun Jin Chun Lack Hyeon Lim Mi Sun Cheong Dongwon Baek Mi Suk Park Hyun Min Cho Su Hyeon Lee Byung Jun Jin Dong Hyeon No Ye Jin Cha Yong Bok Lee Jong Chan Hong Dae-Jin Yun Min Chul Kim |
| author_facet | Hyun Jin Chun Lack Hyeon Lim Mi Sun Cheong Dongwon Baek Mi Suk Park Hyun Min Cho Su Hyeon Lee Byung Jun Jin Dong Hyeon No Ye Jin Cha Yong Bok Lee Jong Chan Hong Dae-Jin Yun Min Chul Kim |
| author_sort | Hyun Jin Chun |
| collection | DOAJ |
| description | Plants possess adaptive reprogramed modules to prolonged environmental stresses, including adjustment of metabolism and gene expression for physiological and morphological adaptation. <i>CCoAOMT1</i> encodes a caffeoyl CoA O-methyltransferase and is known to play an important role in adaptation of <i>Arabidopsis</i> plants to prolonged saline stress. In this study, we showed that the <i>CCoAOMT1</i> gene plays a role in drought stress response. Transcript of <i>CCoAOMT1</i> was induced by salt, dehydration (drought), and methyl viologen (MV), and loss of function mutants of <i>CCoAOMT1</i>, <i>ccoaomt1-1,</i> and <i>ccoaomt1-2</i> exhibit hypersensitive phenotypes to drought and MV stresses. The <i>ccoaomt1</i> mutants accumulated higher level of H<sub>2</sub>O<sub>2</sub> in the leaves and expressed lower levels of drought-responsive genes including <i>RD29B</i>, <i>RD20</i>, <i>RD29A</i>, and <i>ERD1,</i> as well as <i>ABA3 3</i> and <i>NCED3</i> encoding ABA biosynthesis enzymes during drought stress compared to wild-type plants. A seed germination assay of <i>ccoaomt1</i> mutants in the presence of ABA also revealed that <i>CCoAOMT1</i> functions in ABA response. Our data suggests that <i>CCoAOMT1</i> plays a positive role in response to drought stress response by regulating H<sub>2</sub>O<sub>2</sub> accumulation and ABA signaling. |
| first_indexed | 2024-03-10T12:06:55Z |
| format | Article |
| id | doaj.art-a24c1b83d7af45debd4eda8e285cd026 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T12:06:55Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-a24c1b83d7af45debd4eda8e285cd0262023-11-21T16:34:00ZengMDPI AGPlants2223-77472021-04-0110583110.3390/plants10050831<i>Arabidopsis</i> CCoAOMT1 Plays a Role in Drought Stress Response via ROS- and ABA-Dependent MannersHyun Jin Chun0Lack Hyeon Lim1Mi Sun Cheong2Dongwon Baek3Mi Suk Park4Hyun Min Cho5Su Hyeon Lee6Byung Jun Jin7Dong Hyeon No8Ye Jin Cha9Yong Bok Lee10Jong Chan Hong11Dae-Jin Yun12Min Chul Kim13Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, KoreaInstitute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaPlant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, KoreaPlant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, KoreaInstitute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaPlant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaInstitute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaPlants possess adaptive reprogramed modules to prolonged environmental stresses, including adjustment of metabolism and gene expression for physiological and morphological adaptation. <i>CCoAOMT1</i> encodes a caffeoyl CoA O-methyltransferase and is known to play an important role in adaptation of <i>Arabidopsis</i> plants to prolonged saline stress. In this study, we showed that the <i>CCoAOMT1</i> gene plays a role in drought stress response. Transcript of <i>CCoAOMT1</i> was induced by salt, dehydration (drought), and methyl viologen (MV), and loss of function mutants of <i>CCoAOMT1</i>, <i>ccoaomt1-1,</i> and <i>ccoaomt1-2</i> exhibit hypersensitive phenotypes to drought and MV stresses. The <i>ccoaomt1</i> mutants accumulated higher level of H<sub>2</sub>O<sub>2</sub> in the leaves and expressed lower levels of drought-responsive genes including <i>RD29B</i>, <i>RD20</i>, <i>RD29A</i>, and <i>ERD1,</i> as well as <i>ABA3 3</i> and <i>NCED3</i> encoding ABA biosynthesis enzymes during drought stress compared to wild-type plants. A seed germination assay of <i>ccoaomt1</i> mutants in the presence of ABA also revealed that <i>CCoAOMT1</i> functions in ABA response. Our data suggests that <i>CCoAOMT1</i> plays a positive role in response to drought stress response by regulating H<sub>2</sub>O<sub>2</sub> accumulation and ABA signaling.https://www.mdpi.com/2223-7747/10/5/831<i>CCoAOMT1</i>droughtabscisic acid (ABA)reactive oxygen species (ROS) |
| spellingShingle | Hyun Jin Chun Lack Hyeon Lim Mi Sun Cheong Dongwon Baek Mi Suk Park Hyun Min Cho Su Hyeon Lee Byung Jun Jin Dong Hyeon No Ye Jin Cha Yong Bok Lee Jong Chan Hong Dae-Jin Yun Min Chul Kim <i>Arabidopsis</i> CCoAOMT1 Plays a Role in Drought Stress Response via ROS- and ABA-Dependent Manners Plants <i>CCoAOMT1</i> drought abscisic acid (ABA) reactive oxygen species (ROS) |
| title | <i>Arabidopsis</i> CCoAOMT1 Plays a Role in Drought Stress Response via ROS- and ABA-Dependent Manners |
| title_full | <i>Arabidopsis</i> CCoAOMT1 Plays a Role in Drought Stress Response via ROS- and ABA-Dependent Manners |
| title_fullStr | <i>Arabidopsis</i> CCoAOMT1 Plays a Role in Drought Stress Response via ROS- and ABA-Dependent Manners |
| title_full_unstemmed | <i>Arabidopsis</i> CCoAOMT1 Plays a Role in Drought Stress Response via ROS- and ABA-Dependent Manners |
| title_short | <i>Arabidopsis</i> CCoAOMT1 Plays a Role in Drought Stress Response via ROS- and ABA-Dependent Manners |
| title_sort | i arabidopsis i ccoaomt1 plays a role in drought stress response via ros and aba dependent manners |
| topic | <i>CCoAOMT1</i> drought abscisic acid (ABA) reactive oxygen species (ROS) |
| url | https://www.mdpi.com/2223-7747/10/5/831 |
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