Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, <i>Phaseolus acutifolius</i> A. Gray, and Its Wild Relatives
Some of the major impacts of climate change are expected in regions where drought stress is already an issue. Grain legumes are generally drought susceptible. However, tepary bean and its wild relatives within <i>Phaseolus acutifolius</i> or <i>P. parvifolius</i> are from ari...
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MDPI AG
2021-04-01
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author | María A. Buitrago-Bitar Andrés J. Cortés Felipe López-Hernández Jorge M. Londoño-Caicedo Jaime E. Muñoz-Florez L. Carmenza Muñoz Matthew Wohlgemuth Blair |
author_facet | María A. Buitrago-Bitar Andrés J. Cortés Felipe López-Hernández Jorge M. Londoño-Caicedo Jaime E. Muñoz-Florez L. Carmenza Muñoz Matthew Wohlgemuth Blair |
author_sort | María A. Buitrago-Bitar |
collection | DOAJ |
description | Some of the major impacts of climate change are expected in regions where drought stress is already an issue. Grain legumes are generally drought susceptible. However, tepary bean and its wild relatives within <i>Phaseolus acutifolius</i> or <i>P. parvifolius</i> are from arid areas between Mexico and the United States. Therefore, we hypothesize that these bean accessions have diversity signals indicative of adaptation to drought at key candidate genes such as: <i>Asr2</i>, <i>Dreb2B</i>, and <i>ERECTA</i>. By sequencing alleles of these genes and comparing to estimates of drought tolerance indices from climate data for the collection site of geo-referenced, tepary bean accessions, we determined the genotype x environmental association (GEA) of each gene. Diversity analysis found that cultivated and wild <i>P. acutifolius</i> were intermingled with var. <i>tenuifolius</i> and <i>P. parvifolius</i>, signifying that allele diversity was ample in the wild and cultivated clade over a broad sense (<i>sensu lato</i>) evaluation. Genes <i>Dreb2B</i> and <i>ERECTA</i> harbored signatures of directional selection, represented by six SNPs correlated with the environmental drought indices. This suggests that wild tepary bean is a reservoir of novel alleles at genes for drought tolerance, as expected for a species that originated in arid environments. Our study corroborated that candidate gene approach was effective for marker validation across a broad genetic base of wild tepary accessions. |
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spelling | doaj.art-a24c92c76e21404280b05eb7be6c150b2023-11-21T15:09:19ZengMDPI AGGenes2073-44252021-04-0112455610.3390/genes12040556Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, <i>Phaseolus acutifolius</i> A. Gray, and Its Wild RelativesMaría A. Buitrago-Bitar0Andrés J. Cortés1Felipe López-Hernández2Jorge M. Londoño-Caicedo3Jaime E. Muñoz-Florez4L. Carmenza Muñoz5Matthew Wohlgemuth Blair6Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia, Palmira 763533, ColombiaCorporación Colombiana de Investigación Agropecuaria AGROSAVIA, C.I. La Selva, Km 7 vía Rionegro—Las Palmas, Rionegro 054048, ColombiaCorporación Colombiana de Investigación Agropecuaria AGROSAVIA, C.I. La Selva, Km 7 vía Rionegro—Las Palmas, Rionegro 054048, ColombiaFacultad de Ciencias Agropecuarias, Universidad Nacional de Colombia, Palmira 763533, ColombiaFacultad de Ciencias Agropecuarias, Universidad Nacional de Colombia, Palmira 763533, ColombiaFacultad de Ciencias Agropecuarias, Universidad Nacional de Colombia, Palmira 763533, ColombiaFacultad de Ciencias Agropecuarias, Universidad Nacional de Colombia, Palmira 763533, ColombiaSome of the major impacts of climate change are expected in regions where drought stress is already an issue. Grain legumes are generally drought susceptible. However, tepary bean and its wild relatives within <i>Phaseolus acutifolius</i> or <i>P. parvifolius</i> are from arid areas between Mexico and the United States. Therefore, we hypothesize that these bean accessions have diversity signals indicative of adaptation to drought at key candidate genes such as: <i>Asr2</i>, <i>Dreb2B</i>, and <i>ERECTA</i>. By sequencing alleles of these genes and comparing to estimates of drought tolerance indices from climate data for the collection site of geo-referenced, tepary bean accessions, we determined the genotype x environmental association (GEA) of each gene. Diversity analysis found that cultivated and wild <i>P. acutifolius</i> were intermingled with var. <i>tenuifolius</i> and <i>P. parvifolius</i>, signifying that allele diversity was ample in the wild and cultivated clade over a broad sense (<i>sensu lato</i>) evaluation. Genes <i>Dreb2B</i> and <i>ERECTA</i> harbored signatures of directional selection, represented by six SNPs correlated with the environmental drought indices. This suggests that wild tepary bean is a reservoir of novel alleles at genes for drought tolerance, as expected for a species that originated in arid environments. Our study corroborated that candidate gene approach was effective for marker validation across a broad genetic base of wild tepary accessions.https://www.mdpi.com/2073-4425/12/4/556abscisic acid-, stress-, and ripening-induced (<i>Asr</i>) genecandidate gene approachclimate adaptationdehydration responsive element binding (<i>Dreb</i>) genedrought toleranceenvironmental indices |
spellingShingle | María A. Buitrago-Bitar Andrés J. Cortés Felipe López-Hernández Jorge M. Londoño-Caicedo Jaime E. Muñoz-Florez L. Carmenza Muñoz Matthew Wohlgemuth Blair Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, <i>Phaseolus acutifolius</i> A. Gray, and Its Wild Relatives Genes abscisic acid-, stress-, and ripening-induced (<i>Asr</i>) gene candidate gene approach climate adaptation dehydration responsive element binding (<i>Dreb</i>) gene drought tolerance environmental indices |
title | Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, <i>Phaseolus acutifolius</i> A. Gray, and Its Wild Relatives |
title_full | Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, <i>Phaseolus acutifolius</i> A. Gray, and Its Wild Relatives |
title_fullStr | Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, <i>Phaseolus acutifolius</i> A. Gray, and Its Wild Relatives |
title_full_unstemmed | Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, <i>Phaseolus acutifolius</i> A. Gray, and Its Wild Relatives |
title_short | Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, <i>Phaseolus acutifolius</i> A. Gray, and Its Wild Relatives |
title_sort | allelic diversity at abiotic stress responsive genes in relationship to ecological drought indices for cultivated tepary bean i phaseolus acutifolius i a gray and its wild relatives |
topic | abscisic acid-, stress-, and ripening-induced (<i>Asr</i>) gene candidate gene approach climate adaptation dehydration responsive element binding (<i>Dreb</i>) gene drought tolerance environmental indices |
url | https://www.mdpi.com/2073-4425/12/4/556 |
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