White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase
White spot syndrome virus (WSSV) is the causative agent of white spot syndrome, one of the most devastating diseases in shrimp aquaculture. The genome of WSSV includes a gene that encodes a putative family B DNA polymerase (ORF514), which is 16% identical in amino acid sequence to the Herpes virus 1...
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| Materiálatiipa: | Artihkal |
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MDPI AG
2011-01-01
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| Ráidu: | Molecules |
| Fáttát: | |
| Liŋkkat: | http://www.mdpi.com/1420-3049/16/1/532/ |
| _version_ | 1828476127955386368 |
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| author | Rogerio R. Sotelo-Mundo Luis G. Brieba Jesús Hernández Adriana Muhlia-Almazan Gloria Yepiz-Plascencia Aldo A. Arvizu-Flores Karina D. Garcia-Orozco Enrique de-la-Re-Vega |
| author_facet | Rogerio R. Sotelo-Mundo Luis G. Brieba Jesús Hernández Adriana Muhlia-Almazan Gloria Yepiz-Plascencia Aldo A. Arvizu-Flores Karina D. Garcia-Orozco Enrique de-la-Re-Vega |
| author_sort | Rogerio R. Sotelo-Mundo |
| collection | DOAJ |
| description | White spot syndrome virus (WSSV) is the causative agent of white spot syndrome, one of the most devastating diseases in shrimp aquaculture. The genome of WSSV includes a gene that encodes a putative family B DNA polymerase (ORF514), which is 16% identical in amino acid sequence to the Herpes virus 1 DNA polymerase. The aim of this work was to demonstrate the activity of the WSSV ORF514-encoded protein as a DNA polymerase and hence a putative antiviral target. A 3.5 kbp fragment encoding the conserved polymerase and exonuclease domains of ORF514 was overexpressed in bacteria. The recombinant protein showed polymerase activity but with very low level of processivity. Molecular modeling of the catalytic protein core encoded in ORF514 revealed a canonical polymerase fold. Amino acid sequence alignments of ORF514 indicate the presence of a putative PIP box, suggesting that the encoded putative DNA polymerase may use a host processivity factor for optimal activity. We postulate that WSSV ORF514 encodes a bona fide DNA polymerase that requires accessory proteins for activity and maybe target for drugs or compounds that inhibit viral DNA replication. |
| first_indexed | 2024-12-11T06:24:13Z |
| format | Article |
| id | doaj.art-a24da4939a274be78f351eefc9a530e8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T06:24:13Z |
| publishDate | 2011-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a24da4939a274be78f351eefc9a530e82022-12-22T01:17:44ZengMDPI AGMolecules1420-30492011-01-0116153254210.3390/molecules16010532White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA PolymeraseRogerio R. Sotelo-MundoLuis G. BriebaJesús HernándezAdriana Muhlia-AlmazanGloria Yepiz-PlascenciaAldo A. Arvizu-FloresKarina D. Garcia-OrozcoEnrique de-la-Re-VegaWhite spot syndrome virus (WSSV) is the causative agent of white spot syndrome, one of the most devastating diseases in shrimp aquaculture. The genome of WSSV includes a gene that encodes a putative family B DNA polymerase (ORF514), which is 16% identical in amino acid sequence to the Herpes virus 1 DNA polymerase. The aim of this work was to demonstrate the activity of the WSSV ORF514-encoded protein as a DNA polymerase and hence a putative antiviral target. A 3.5 kbp fragment encoding the conserved polymerase and exonuclease domains of ORF514 was overexpressed in bacteria. The recombinant protein showed polymerase activity but with very low level of processivity. Molecular modeling of the catalytic protein core encoded in ORF514 revealed a canonical polymerase fold. Amino acid sequence alignments of ORF514 indicate the presence of a putative PIP box, suggesting that the encoded putative DNA polymerase may use a host processivity factor for optimal activity. We postulate that WSSV ORF514 encodes a bona fide DNA polymerase that requires accessory proteins for activity and maybe target for drugs or compounds that inhibit viral DNA replication.http://www.mdpi.com/1420-3049/16/1/532/DNA polymerase family BWSSVwhite spot syndrome viruspolymerase activityCrustaceashrimpprawnLitopenaeus vannameiDecapodainvertebrate |
| spellingShingle | Rogerio R. Sotelo-Mundo Luis G. Brieba Jesús Hernández Adriana Muhlia-Almazan Gloria Yepiz-Plascencia Aldo A. Arvizu-Flores Karina D. Garcia-Orozco Enrique de-la-Re-Vega White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase Molecules DNA polymerase family B WSSV white spot syndrome virus polymerase activity Crustacea shrimp prawn Litopenaeus vannamei Decapoda invertebrate |
| title | White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase |
| title_full | White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase |
| title_fullStr | White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase |
| title_full_unstemmed | White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase |
| title_short | White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase |
| title_sort | white spot syndrome virus orf514 encodes a bona fide dna polymerase |
| topic | DNA polymerase family B WSSV white spot syndrome virus polymerase activity Crustacea shrimp prawn Litopenaeus vannamei Decapoda invertebrate |
| url | http://www.mdpi.com/1420-3049/16/1/532/ |
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