The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex
Summary: There is an urgent need for a molecular understanding of how SARS-CoV-2 influences the machineries of the host cell. Herein, we focused our attention on the capacity of the SARS-CoV-2 protein NSP2 to bind the human 4EHP-GIGYF2 complex, a key factor involved in microRNA-mediated silencing of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422200918X |
| _version_ | 1818245288275476480 |
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| author | Limei Zou Clara Moch Marc Graille Clément Chapat |
| author_facet | Limei Zou Clara Moch Marc Graille Clément Chapat |
| author_sort | Limei Zou |
| collection | DOAJ |
| description | Summary: There is an urgent need for a molecular understanding of how SARS-CoV-2 influences the machineries of the host cell. Herein, we focused our attention on the capacity of the SARS-CoV-2 protein NSP2 to bind the human 4EHP-GIGYF2 complex, a key factor involved in microRNA-mediated silencing of gene expression. Using in vitro interaction assays, our data demonstrate that NSP2 physically associates with both 4EHP and a central segment in GIGYF2 in the cytoplasm. We also provide functional evidence showing that NSP2 impairs the function of GIGYF2 in mediating translation repression using reporter-based assays. Collectively, these data reveal the potential impact of NSP2 on the post-transcriptional silencing of gene expression in human cells, pointing out 4EHP-GIGYF2 targeting as a possible strategy of SARS-CoV-2 to take over the silencing machinery and to suppress host defenses. |
| first_indexed | 2024-12-12T14:30:32Z |
| format | Article |
| id | doaj.art-a250a7f1f9014afb979ba32ad2b790a4 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-12T14:30:32Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-a250a7f1f9014afb979ba32ad2b790a42022-12-22T00:21:34ZengElsevieriScience2589-00422022-07-01257104646The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complexLimei Zou0Clara Moch1Marc Graille2Clément Chapat3Laboratoire de Biologie Structurale de La Cellule (BIOC), CNRS, Ecole Polytechnique, IP Paris, 91128 Palaiseau, FranceLaboratoire de Biologie Structurale de La Cellule (BIOC), CNRS, Ecole Polytechnique, IP Paris, 91128 Palaiseau, FranceLaboratoire de Biologie Structurale de La Cellule (BIOC), CNRS, Ecole Polytechnique, IP Paris, 91128 Palaiseau, FranceLaboratoire de Biologie Structurale de La Cellule (BIOC), CNRS, Ecole Polytechnique, IP Paris, 91128 Palaiseau, France; Corresponding authorSummary: There is an urgent need for a molecular understanding of how SARS-CoV-2 influences the machineries of the host cell. Herein, we focused our attention on the capacity of the SARS-CoV-2 protein NSP2 to bind the human 4EHP-GIGYF2 complex, a key factor involved in microRNA-mediated silencing of gene expression. Using in vitro interaction assays, our data demonstrate that NSP2 physically associates with both 4EHP and a central segment in GIGYF2 in the cytoplasm. We also provide functional evidence showing that NSP2 impairs the function of GIGYF2 in mediating translation repression using reporter-based assays. Collectively, these data reveal the potential impact of NSP2 on the post-transcriptional silencing of gene expression in human cells, pointing out 4EHP-GIGYF2 targeting as a possible strategy of SARS-CoV-2 to take over the silencing machinery and to suppress host defenses.http://www.sciencedirect.com/science/article/pii/S258900422200918XVirologyMolecular biology |
| spellingShingle | Limei Zou Clara Moch Marc Graille Clément Chapat The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex iScience Virology Molecular biology |
| title | The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex |
| title_full | The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex |
| title_fullStr | The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex |
| title_full_unstemmed | The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex |
| title_short | The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex |
| title_sort | sars cov 2 protein nsp2 impairs the silencing capacity of the human 4ehp gigyf2 complex |
| topic | Virology Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S258900422200918X |
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