MFAP2 promotes the progression of oral squamous cell carcinoma by activating the Wnt/β-catenin signaling pathway through autophagy
Microfibrillar-associated protein 2 (MFAP2) is a small glycoprotein that is involved in vascular development and metabolic disease. The present study aims to explore the regulatory role of MFAP2 in the development and progression of oral sq...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2023-08-01
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| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2023079 |
| _version_ | 1797635914707501056 |
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| author | Zhang Hao Shen Si Feng Chong Chen Gang Wang Xinxing |
| author_facet | Zhang Hao Shen Si Feng Chong Chen Gang Wang Xinxing |
| author_sort | Zhang Hao |
| collection | DOAJ |
| description | Microfibrillar-associated protein 2 (MFAP2) is a small glycoprotein that is involved in vascular development and metabolic disease. The present study aims to explore the regulatory role of MFAP2 in the development and progression of oral squamous cell carcinoma (OSCC), including the underlying mechanisms. MFAP2 expression and its association with the progression of OSCC are explored using bioinformatics. MFAP2 expression in OSCC tissues is detected by immunohistochemical staining. SCC15 cell migration, invasion, apoptosis, proliferation, and viability are detected by wound healing, Transwell, flow cytometry, colony formation, and cell counting kit-8 assays. An in vivo experiment is used to detect tumor formation. Western blot analysis is used to determine MFAP2’s regulatory role in autophagy and the Wnt/β-catenin signaling pathway. MFAP2 is highly expressed in SCC15 cells and OSCC tissues, which correlates positively with the poor prognosis of patients with OSCCs. Functionally, MFAP2 promotes oncogenic autophagy to increase cell invasion, migration, and proliferation but inhibits apoptosis in SCC15 cells and promotes tumor growth in vivo. Mechanistically, MFAP2 upregulates autophagy and Wnt/β-catenin signaling to stimulate OSCC development. Intriguingly, regulation of Wnt/β-catenin signaling dependent on autophagy contributes to the malignant behaviors of SCC15 cells. MFAP2 could serve as a novel biomarker for OSCC and could affect OSCC tumorigenesis and development via autophagic regulation of Wnt/β-catenin signaling. |
| first_indexed | 2024-03-11T12:26:54Z |
| format | Article |
| id | doaj.art-a2557b00aaf74123bd9b4c704cba00a3 |
| institution | Directory Open Access Journal |
| issn | 1672-9145 |
| language | English |
| last_indexed | 2024-03-11T12:26:54Z |
| publishDate | 2023-08-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj.art-a2557b00aaf74123bd9b4c704cba00a32023-11-06T08:30:46ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452023-08-01551445145510.3724/abbs.202307920d259ccMFAP2 promotes the progression of oral squamous cell carcinoma by activating the Wnt/β-catenin signaling pathway through autophagyZhang Hao0Shen Si1Feng Chong2Chen Gang3Wang Xinxing4["Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China","School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China"]["Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China","School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China"]["Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China","School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China"]["School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China"]["Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China"]Microfibrillar-associated protein 2 (MFAP2) is a small glycoprotein that is involved in vascular development and metabolic disease. The present study aims to explore the regulatory role of MFAP2 in the development and progression of oral squamous cell carcinoma (OSCC), including the underlying mechanisms. MFAP2 expression and its association with the progression of OSCC are explored using bioinformatics. MFAP2 expression in OSCC tissues is detected by immunohistochemical staining. SCC15 cell migration, invasion, apoptosis, proliferation, and viability are detected by wound healing, Transwell, flow cytometry, colony formation, and cell counting kit-8 assays. An in vivo experiment is used to detect tumor formation. Western blot analysis is used to determine MFAP2’s regulatory role in autophagy and the Wnt/β-catenin signaling pathway. MFAP2 is highly expressed in SCC15 cells and OSCC tissues, which correlates positively with the poor prognosis of patients with OSCCs. Functionally, MFAP2 promotes oncogenic autophagy to increase cell invasion, migration, and proliferation but inhibits apoptosis in SCC15 cells and promotes tumor growth in vivo. Mechanistically, MFAP2 upregulates autophagy and Wnt/β-catenin signaling to stimulate OSCC development. Intriguingly, regulation of Wnt/β-catenin signaling dependent on autophagy contributes to the malignant behaviors of SCC15 cells. MFAP2 could serve as a novel biomarker for OSCC and could affect OSCC tumorigenesis and development via autophagic regulation of Wnt/β-catenin signaling.https://www.sciengine.com/doi/10.3724/abbs.2023079oral squamous cell carcinomamicrofibrillar-associated protein 2autophagyWnt/β-catenin pathway<italic>in vivo</italic> |
| spellingShingle | Zhang Hao Shen Si Feng Chong Chen Gang Wang Xinxing MFAP2 promotes the progression of oral squamous cell carcinoma by activating the Wnt/β-catenin signaling pathway through autophagy Acta Biochimica et Biophysica Sinica oral squamous cell carcinoma microfibrillar-associated protein 2 autophagy Wnt/β-catenin pathway <italic>in vivo</italic> |
| title | MFAP2 promotes the progression of oral squamous cell carcinoma by activating the Wnt/β-catenin signaling pathway through autophagy |
| title_full | MFAP2 promotes the progression of oral squamous cell carcinoma by activating the Wnt/β-catenin signaling pathway through autophagy |
| title_fullStr | MFAP2 promotes the progression of oral squamous cell carcinoma by activating the Wnt/β-catenin signaling pathway through autophagy |
| title_full_unstemmed | MFAP2 promotes the progression of oral squamous cell carcinoma by activating the Wnt/β-catenin signaling pathway through autophagy |
| title_short | MFAP2 promotes the progression of oral squamous cell carcinoma by activating the Wnt/β-catenin signaling pathway through autophagy |
| title_sort | mfap2 promotes the progression of oral squamous cell carcinoma by activating the wnt β catenin signaling pathway through autophagy |
| topic | oral squamous cell carcinoma microfibrillar-associated protein 2 autophagy Wnt/β-catenin pathway <italic>in vivo</italic> |
| url | https://www.sciengine.com/doi/10.3724/abbs.2023079 |
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