miRNA regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenol
<p>Abstract</p> <p>Background</p> <p>It is known that some environmental chemicals affect the human endocrine system. The harmful effects of endocrine disrupting chemical (EDC) nonylphenol (NP) have been studied since the 1980s. It is known that NP adversely affects phy...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-09-01
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| Series: | Reproductive Biology and Endocrinology |
| Online Access: | http://www.rbej.com/content/9/1/126 |
| _version_ | 1811244035720871936 |
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| author | Kim Seung Jun Oh Moon-Ju Kang Seung-Jun Park Se-Myo Choi Mi-Sun Park Han-Jin Oh Jung-Hwa Choi Jin-Sung Hwang Seung Yong Yoon Seokjoo |
| author_facet | Kim Seung Jun Oh Moon-Ju Kang Seung-Jun Park Se-Myo Choi Mi-Sun Park Han-Jin Oh Jung-Hwa Choi Jin-Sung Hwang Seung Yong Yoon Seokjoo |
| author_sort | Kim Seung Jun |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>It is known that some environmental chemicals affect the human endocrine system. The harmful effects of endocrine disrupting chemical (EDC) nonylphenol (NP) have been studied since the 1980s. It is known that NP adversely affects physiological functions by mimicking the natural hormone 17 beta-estradiol. In the present study, we analyzed the expression of miRNAs and their target genes in mouse Sertoli TM4 cells to better understand the regulatory roles of miRNAs on Sertoli cells after NP exposure.</p> <p>Methods</p> <p>Mouse TM4 Sertoli cells were treated with NP for 3 or 24 h, and global gene and miRNA expression were analyzed using Agilent mouse whole genome and mouse miRNA v13 arrays.</p> <p>Results</p> <p>We identified genes that were > 2-fold differentially expressed in NP-treated cells and control cells (<it>P </it>< 0.05) and analyzed their functions through Gene Ontology analysis. We also identified miRNAs that were differentially expressed in NP-treated and control cells. Of the 186 miRNAs the expression of which differed between NP-treated and control cells, 59 and 147 miRNAs exhibited 1.3-fold increased or decreased expression at 3 and 24 h, respectively. Network analysis of deregulated miRNAs suggested that <it>Ppara </it>may regulate the expression of certain miRNAs, including miR-378, miR-125a-3p miR-20a, miR-203, and miR-101a, after exposure to NP. Additionally, comprehensive analysis of predicted target genes for miRNAs showed that the expression of genes with roles in cell proliferation, the cell cycle, and cell death were regulated by miRNA in NP-treated TM4 cells. Levels of expression of the miRNAs miR-135a* and miR-199a-5p were validated by qRT-PCR. Finally, miR-135a* target gene analysis suggests that the generation of reactive oxygen species (ROS) following exposure to NP exposure may be mediated by miR-135a* through regulation of the Wnt/beta-catenin signaling pathway.</p> <p>Conclusions</p> <p>Collectively, these data help to determine NP's actions on mouse TM4 Sertoli cells and increase our understanding of the molecular mechanisms underlying the adverse effects of xenoestrogens on the reproductive system.</p> |
| first_indexed | 2024-04-12T14:18:55Z |
| format | Article |
| id | doaj.art-a255f9923e3541e6ac653549284066bd |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-04-12T14:18:55Z |
| publishDate | 2011-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-a255f9923e3541e6ac653549284066bd2022-12-22T03:29:39ZengBMCReproductive Biology and Endocrinology1477-78272011-09-019112610.1186/1477-7827-9-126miRNA regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenolKim Seung JunOh Moon-JuKang Seung-JunPark Se-MyoChoi Mi-SunPark Han-JinOh Jung-HwaChoi Jin-SungHwang Seung YongYoon Seokjoo<p>Abstract</p> <p>Background</p> <p>It is known that some environmental chemicals affect the human endocrine system. The harmful effects of endocrine disrupting chemical (EDC) nonylphenol (NP) have been studied since the 1980s. It is known that NP adversely affects physiological functions by mimicking the natural hormone 17 beta-estradiol. In the present study, we analyzed the expression of miRNAs and their target genes in mouse Sertoli TM4 cells to better understand the regulatory roles of miRNAs on Sertoli cells after NP exposure.</p> <p>Methods</p> <p>Mouse TM4 Sertoli cells were treated with NP for 3 or 24 h, and global gene and miRNA expression were analyzed using Agilent mouse whole genome and mouse miRNA v13 arrays.</p> <p>Results</p> <p>We identified genes that were > 2-fold differentially expressed in NP-treated cells and control cells (<it>P </it>< 0.05) and analyzed their functions through Gene Ontology analysis. We also identified miRNAs that were differentially expressed in NP-treated and control cells. Of the 186 miRNAs the expression of which differed between NP-treated and control cells, 59 and 147 miRNAs exhibited 1.3-fold increased or decreased expression at 3 and 24 h, respectively. Network analysis of deregulated miRNAs suggested that <it>Ppara </it>may regulate the expression of certain miRNAs, including miR-378, miR-125a-3p miR-20a, miR-203, and miR-101a, after exposure to NP. Additionally, comprehensive analysis of predicted target genes for miRNAs showed that the expression of genes with roles in cell proliferation, the cell cycle, and cell death were regulated by miRNA in NP-treated TM4 cells. Levels of expression of the miRNAs miR-135a* and miR-199a-5p were validated by qRT-PCR. Finally, miR-135a* target gene analysis suggests that the generation of reactive oxygen species (ROS) following exposure to NP exposure may be mediated by miR-135a* through regulation of the Wnt/beta-catenin signaling pathway.</p> <p>Conclusions</p> <p>Collectively, these data help to determine NP's actions on mouse TM4 Sertoli cells and increase our understanding of the molecular mechanisms underlying the adverse effects of xenoestrogens on the reproductive system.</p>http://www.rbej.com/content/9/1/126 |
| spellingShingle | Kim Seung Jun Oh Moon-Ju Kang Seung-Jun Park Se-Myo Choi Mi-Sun Park Han-Jin Oh Jung-Hwa Choi Jin-Sung Hwang Seung Yong Yoon Seokjoo miRNA regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenol Reproductive Biology and Endocrinology |
| title | miRNA regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenol |
| title_full | miRNA regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenol |
| title_fullStr | miRNA regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenol |
| title_full_unstemmed | miRNA regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenol |
| title_short | miRNA regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenol |
| title_sort | mirna regulation of cytotoxic effects in mouse sertoli cells exposed to nonylphenol |
| url | http://www.rbej.com/content/9/1/126 |
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