Planning and projecting of a green isoindole-based fluoro-probe for feasible tagging and tracking of topiramate, a non-fluorescent drug in bulk powder and prescribed commercial products
Fluorescent probes have supplanted more conventional techniques in many experiments due to their versatility, sensitivity, and measurability. Topiramate, the objective compound lacks fluorogenic or chromophoric moieties rendering its direct analysis impractical. Without these groups, the molecule la...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Talanta Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666831923000267 |
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| author | Ahmed Abdulhafez Hamad Afnan S. Batubara |
| author_facet | Ahmed Abdulhafez Hamad Afnan S. Batubara |
| author_sort | Ahmed Abdulhafez Hamad |
| collection | DOAJ |
| description | Fluorescent probes have supplanted more conventional techniques in many experiments due to their versatility, sensitivity, and measurability. Topiramate, the objective compound lacks fluorogenic or chromophoric moieties rendering its direct analysis impractical. Without these groups, the molecule lacks the ability to fluoresce or absorb light in its natural state when subjected to a source of ultraviolet radiation. Tagging with a tracking reagent or fluorescent probing can overcome this phenomenon. Under the specific conditions In this study, a new, eco-friendly, practically applicable, and specific fluorometric approach to the assay of none fluorescent drug, topiramate was proposed. The planned analytical approach is dependent on the integration of fluorescence probes through the use of the o-phthalaldehyde reagent to yield a potent fluorescent product that can be measured fluorimetrically. Topiramate's unsubstituted amine moiety can condense with o-phthalaldehyde and thiol moiety in a buffered solution. After being excited at 339 nm. the product was measured at em 440 nm. This technique provides linearity within a concentration scale of 0.1–0.9 µg mL−1. The system quantum yield was evaluated by a comparative method. Also, the probe validation was analyzed in accordance with the standards set by the International Council for Harmonization (ICH). Excipients had no appreciable impact on the proposed probe's performance when testing topiramate in both batch powder and commercially available products. The results show remarkable consistency with the documented reference method with no obvious discrepancies between the two. Finally, the planned system was evaluated from the environmental side through different ecological metrics such as National Environmental Methods Index (NEMI), Eco-Scale Assessment (ESA), Green Analytical Procedure Index (GAPI), and Analytical GREEnness measure (AGREE) and showed a high degree of greenness (ES: 93/100; AGREE 0.75/1 and full green NEMI pictograms). |
| first_indexed | 2024-03-13T07:17:18Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2666-8319 |
| language | English |
| last_indexed | 2024-03-13T07:17:18Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
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| series | Talanta Open |
| spelling | doaj.art-a256c9cb60bf46a38a10b77393aba5002023-06-05T04:13:17ZengElsevierTalanta Open2666-83192023-08-017100205Planning and projecting of a green isoindole-based fluoro-probe for feasible tagging and tracking of topiramate, a non-fluorescent drug in bulk powder and prescribed commercial productsAhmed Abdulhafez Hamad0Afnan S. Batubara1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt; Corresponding author.Department of Pharmaceutical Chemistry, College of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi ArabiaFluorescent probes have supplanted more conventional techniques in many experiments due to their versatility, sensitivity, and measurability. Topiramate, the objective compound lacks fluorogenic or chromophoric moieties rendering its direct analysis impractical. Without these groups, the molecule lacks the ability to fluoresce or absorb light in its natural state when subjected to a source of ultraviolet radiation. Tagging with a tracking reagent or fluorescent probing can overcome this phenomenon. Under the specific conditions In this study, a new, eco-friendly, practically applicable, and specific fluorometric approach to the assay of none fluorescent drug, topiramate was proposed. The planned analytical approach is dependent on the integration of fluorescence probes through the use of the o-phthalaldehyde reagent to yield a potent fluorescent product that can be measured fluorimetrically. Topiramate's unsubstituted amine moiety can condense with o-phthalaldehyde and thiol moiety in a buffered solution. After being excited at 339 nm. the product was measured at em 440 nm. This technique provides linearity within a concentration scale of 0.1–0.9 µg mL−1. The system quantum yield was evaluated by a comparative method. Also, the probe validation was analyzed in accordance with the standards set by the International Council for Harmonization (ICH). Excipients had no appreciable impact on the proposed probe's performance when testing topiramate in both batch powder and commercially available products. The results show remarkable consistency with the documented reference method with no obvious discrepancies between the two. Finally, the planned system was evaluated from the environmental side through different ecological metrics such as National Environmental Methods Index (NEMI), Eco-Scale Assessment (ESA), Green Analytical Procedure Index (GAPI), and Analytical GREEnness measure (AGREE) and showed a high degree of greenness (ES: 93/100; AGREE 0.75/1 and full green NEMI pictograms).http://www.sciencedirect.com/science/article/pii/S2666831923000267Fluorescent probeTopiramateO-phthalaldehydeCommercial productsEnvironmental impact assessment |
| spellingShingle | Ahmed Abdulhafez Hamad Afnan S. Batubara Planning and projecting of a green isoindole-based fluoro-probe for feasible tagging and tracking of topiramate, a non-fluorescent drug in bulk powder and prescribed commercial products Talanta Open Fluorescent probe Topiramate O-phthalaldehyde Commercial products Environmental impact assessment |
| title | Planning and projecting of a green isoindole-based fluoro-probe for feasible tagging and tracking of topiramate, a non-fluorescent drug in bulk powder and prescribed commercial products |
| title_full | Planning and projecting of a green isoindole-based fluoro-probe for feasible tagging and tracking of topiramate, a non-fluorescent drug in bulk powder and prescribed commercial products |
| title_fullStr | Planning and projecting of a green isoindole-based fluoro-probe for feasible tagging and tracking of topiramate, a non-fluorescent drug in bulk powder and prescribed commercial products |
| title_full_unstemmed | Planning and projecting of a green isoindole-based fluoro-probe for feasible tagging and tracking of topiramate, a non-fluorescent drug in bulk powder and prescribed commercial products |
| title_short | Planning and projecting of a green isoindole-based fluoro-probe for feasible tagging and tracking of topiramate, a non-fluorescent drug in bulk powder and prescribed commercial products |
| title_sort | planning and projecting of a green isoindole based fluoro probe for feasible tagging and tracking of topiramate a non fluorescent drug in bulk powder and prescribed commercial products |
| topic | Fluorescent probe Topiramate O-phthalaldehyde Commercial products Environmental impact assessment |
| url | http://www.sciencedirect.com/science/article/pii/S2666831923000267 |
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