Antimicrobial Efficacy of a Portable UV-C-Based Coating Activation Device against <i>Candida albicans</i> Biofilm and SARS-CoV-2 as an Additional Feature: An In Vitro Study
This in vitro study aimed to confirm the effect of the additional features of Kirei Keep Light (KKL), a commercial UV-C irradiation system that was originally created for coating the surface of removable dentures with photoreactive 2-methacryloyloxyethyl phosphorylcholine (MPC), on the antimicrobial...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Hygiene |
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| Online Access: | https://www.mdpi.com/2673-947X/4/1/6 |
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| author | Adityakrisna Yoshi Putra Wigianto Megumi Watanabe Yuki Iwawaki Takaharu Goto Tamaki Otsuki Tetsuo Ichikawa |
| author_facet | Adityakrisna Yoshi Putra Wigianto Megumi Watanabe Yuki Iwawaki Takaharu Goto Tamaki Otsuki Tetsuo Ichikawa |
| author_sort | Adityakrisna Yoshi Putra Wigianto |
| collection | DOAJ |
| description | This in vitro study aimed to confirm the effect of the additional features of Kirei Keep Light (KKL), a commercial UV-C irradiation system that was originally created for coating the surface of removable dentures with photoreactive 2-methacryloyloxyethyl phosphorylcholine (MPC), on the antimicrobial efficacy aspect against <i>Candida albicans</i> biofilm on a denture base material (PMMA) and SARS-CoV-2. Materials and Methods: The antimicrobial efficacy on <i>C. albicans</i> biofilm was evaluated through quantitative (CFU) and qualitative (SEM images) analysis of three groups: no treatment (control), KKL, and immersion in a disinfectant solution, MCAE. The quantitative evaluation on SARS-CoV-2 was performed by comparing the untreated (control) group and the KKL group. Results: In comparison with the control group (2.39 × 10<sup>6</sup> CFU/mL), KKL irradiation resulted in a 91.01% reduction in <i>C. albicans</i> biofilm (2.15 × 10<sup>5</sup> CFU/mL), whereas for the MCAE group, this reduction was 99.98% (4.64 × 10<sup>2</sup> CFU/mL). The SEM image results also corroborate the CFU results, which showed that the fewest clean surfaces were found in the control, and this gradually increased with KKL and MCAE. SARS-CoV-2 inhibition, indicated by its TCID50 value, demonstrated that KKL almost completely inhibited SARS-CoV-2 infection and replication (99.99% reduction). Conclusion: KKL possesses antimicrobial efficacy on <i>C. albicans</i> biofilm on PMMA and SARS-CoV-2. |
| first_indexed | 2024-04-24T18:12:38Z |
| format | Article |
| id | doaj.art-a25b8e62a1a04aa89bb2cc11864a81f7 |
| institution | Directory Open Access Journal |
| issn | 2673-947X |
| language | English |
| last_indexed | 2024-04-24T18:12:38Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Hygiene |
| spelling | doaj.art-a25b8e62a1a04aa89bb2cc11864a81f72024-03-27T13:44:44ZengMDPI AGHygiene2673-947X2024-02-01419310210.3390/hygiene4010006Antimicrobial Efficacy of a Portable UV-C-Based Coating Activation Device against <i>Candida albicans</i> Biofilm and SARS-CoV-2 as an Additional Feature: An In Vitro StudyAdityakrisna Yoshi Putra Wigianto0Megumi Watanabe1Yuki Iwawaki2Takaharu Goto3Tamaki Otsuki4Tetsuo Ichikawa5Department of Prosthodontics and Oral Rehabilitation, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 770-8504, JapanDepartment of Prosthodontics and Oral Rehabilitation, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 770-8504, JapanDepartment of Prosthodontics and Oral Rehabilitation, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 770-8504, JapanDepartment of Prosthodontics and Oral Rehabilitation, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 770-8504, JapanSun Medical Company, Moriyama 524-0044, JapanDepartment of Prosthodontics and Oral Rehabilitation, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 770-8504, JapanThis in vitro study aimed to confirm the effect of the additional features of Kirei Keep Light (KKL), a commercial UV-C irradiation system that was originally created for coating the surface of removable dentures with photoreactive 2-methacryloyloxyethyl phosphorylcholine (MPC), on the antimicrobial efficacy aspect against <i>Candida albicans</i> biofilm on a denture base material (PMMA) and SARS-CoV-2. Materials and Methods: The antimicrobial efficacy on <i>C. albicans</i> biofilm was evaluated through quantitative (CFU) and qualitative (SEM images) analysis of three groups: no treatment (control), KKL, and immersion in a disinfectant solution, MCAE. The quantitative evaluation on SARS-CoV-2 was performed by comparing the untreated (control) group and the KKL group. Results: In comparison with the control group (2.39 × 10<sup>6</sup> CFU/mL), KKL irradiation resulted in a 91.01% reduction in <i>C. albicans</i> biofilm (2.15 × 10<sup>5</sup> CFU/mL), whereas for the MCAE group, this reduction was 99.98% (4.64 × 10<sup>2</sup> CFU/mL). The SEM image results also corroborate the CFU results, which showed that the fewest clean surfaces were found in the control, and this gradually increased with KKL and MCAE. SARS-CoV-2 inhibition, indicated by its TCID50 value, demonstrated that KKL almost completely inhibited SARS-CoV-2 infection and replication (99.99% reduction). Conclusion: KKL possesses antimicrobial efficacy on <i>C. albicans</i> biofilm on PMMA and SARS-CoV-2.https://www.mdpi.com/2673-947X/4/1/6UV-C irradiationMPC coating<i>Candida albicans</i>SARS-CoV-2 |
| spellingShingle | Adityakrisna Yoshi Putra Wigianto Megumi Watanabe Yuki Iwawaki Takaharu Goto Tamaki Otsuki Tetsuo Ichikawa Antimicrobial Efficacy of a Portable UV-C-Based Coating Activation Device against <i>Candida albicans</i> Biofilm and SARS-CoV-2 as an Additional Feature: An In Vitro Study Hygiene UV-C irradiation MPC coating <i>Candida albicans</i> SARS-CoV-2 |
| title | Antimicrobial Efficacy of a Portable UV-C-Based Coating Activation Device against <i>Candida albicans</i> Biofilm and SARS-CoV-2 as an Additional Feature: An In Vitro Study |
| title_full | Antimicrobial Efficacy of a Portable UV-C-Based Coating Activation Device against <i>Candida albicans</i> Biofilm and SARS-CoV-2 as an Additional Feature: An In Vitro Study |
| title_fullStr | Antimicrobial Efficacy of a Portable UV-C-Based Coating Activation Device against <i>Candida albicans</i> Biofilm and SARS-CoV-2 as an Additional Feature: An In Vitro Study |
| title_full_unstemmed | Antimicrobial Efficacy of a Portable UV-C-Based Coating Activation Device against <i>Candida albicans</i> Biofilm and SARS-CoV-2 as an Additional Feature: An In Vitro Study |
| title_short | Antimicrobial Efficacy of a Portable UV-C-Based Coating Activation Device against <i>Candida albicans</i> Biofilm and SARS-CoV-2 as an Additional Feature: An In Vitro Study |
| title_sort | antimicrobial efficacy of a portable uv c based coating activation device against i candida albicans i biofilm and sars cov 2 as an additional feature an in vitro study |
| topic | UV-C irradiation MPC coating <i>Candida albicans</i> SARS-CoV-2 |
| url | https://www.mdpi.com/2673-947X/4/1/6 |
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