P62/Ubiquitin IHC expression in gastrointestinal carcinomas
P62 and ubiquitin are small regulatory proteins demonstrated to have implications in the prognosis and survival of various malignancies including: hepatocellular, breast, ovarian, and some gastrointestinal carcinomas. Several trials studied the link of their activity to the extrinsic apoptosis pathw...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-03-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fonc.2015.00070/full |
| _version_ | 1811195772916465664 |
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| author | Amr eMohamed Amr eMohamed Johnson eDeniece Charles eKovach Momin T. Siddiqui Cynthia eCohen |
| author_facet | Amr eMohamed Amr eMohamed Johnson eDeniece Charles eKovach Momin T. Siddiqui Cynthia eCohen |
| author_sort | Amr eMohamed |
| collection | DOAJ |
| description | P62 and ubiquitin are small regulatory proteins demonstrated to have implications in the prognosis and survival of various malignancies including: hepatocellular, breast, ovarian, and some gastrointestinal carcinomas. Several trials studied the link of their activity to the extrinsic apoptosis pathway and showed that their autophagy modification has a critical stand point in tumorigenesis. These findings explain their vital role in controlling the process of cell death and survival. It has been shown recently that p62 and ubiquitin overexpression in different types of cancers, such as triple negative breast and ovarian cancers, have directly correlated with incidence of distant metastases. We aim to evaluate p62/ubiquitin expression in gastrointestinal carcinomas of gastric, colonic and pancreatic origin. In gastric carcinoma (45), positive p62 nuclear expression was noted in 53% and cytoplasmic in 57%, while positive ubiquitin was nuclear expressed in 80%, and cytoplasmic in 24%. In colon carcinoma (70), positive p62 nuclear expression was noted in 41% and cytoplasmic in 68.5%, while positive ubiquitin was nuclear in 57% and cytoplasmic in 42%. In pancreatic cancer, positive p62 nuclear expression was noted in 86% and cytoplasmic in 60%, while positive ubiquitin was nuclear in 100% and cytoplasmic in 80%. Normal gastric (6), colon (4) and pancreatic (4) tissues were negative for both P62 and ubiquitin (nuclear and cytoplasmic staining <20%). The results suggest that p62 and ubiquitin are highly expressed in nuclei and cytoplasm of gastric, colonic and pancreatic carcinomas. More studies are needed to correlate IHC expression of p62/ubiquitin with clinicopathologic parameters and overall survival in GI carcinomas. |
| first_indexed | 2024-04-12T00:49:32Z |
| format | Article |
| id | doaj.art-a25c157f66e14f6c80fb61588f75b9d0 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-12T00:49:32Z |
| publishDate | 2015-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-a25c157f66e14f6c80fb61588f75b9d02022-12-22T03:54:47ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2015-03-01510.3389/fonc.2015.00070111231P62/Ubiquitin IHC expression in gastrointestinal carcinomasAmr eMohamed0Amr eMohamed1Johnson eDeniece2Charles eKovach3Momin T. Siddiqui4Cynthia eCohen5Emory University School of MedicineMorehouse School of MedicineMorehouse School of MedicineEmory University School of MedicineEmory University School of MedicineEmory University School of MedicineP62 and ubiquitin are small regulatory proteins demonstrated to have implications in the prognosis and survival of various malignancies including: hepatocellular, breast, ovarian, and some gastrointestinal carcinomas. Several trials studied the link of their activity to the extrinsic apoptosis pathway and showed that their autophagy modification has a critical stand point in tumorigenesis. These findings explain their vital role in controlling the process of cell death and survival. It has been shown recently that p62 and ubiquitin overexpression in different types of cancers, such as triple negative breast and ovarian cancers, have directly correlated with incidence of distant metastases. We aim to evaluate p62/ubiquitin expression in gastrointestinal carcinomas of gastric, colonic and pancreatic origin. In gastric carcinoma (45), positive p62 nuclear expression was noted in 53% and cytoplasmic in 57%, while positive ubiquitin was nuclear expressed in 80%, and cytoplasmic in 24%. In colon carcinoma (70), positive p62 nuclear expression was noted in 41% and cytoplasmic in 68.5%, while positive ubiquitin was nuclear in 57% and cytoplasmic in 42%. In pancreatic cancer, positive p62 nuclear expression was noted in 86% and cytoplasmic in 60%, while positive ubiquitin was nuclear in 100% and cytoplasmic in 80%. Normal gastric (6), colon (4) and pancreatic (4) tissues were negative for both P62 and ubiquitin (nuclear and cytoplasmic staining <20%). The results suggest that p62 and ubiquitin are highly expressed in nuclei and cytoplasm of gastric, colonic and pancreatic carcinomas. More studies are needed to correlate IHC expression of p62/ubiquitin with clinicopathologic parameters and overall survival in GI carcinomas.http://journal.frontiersin.org/Journal/10.3389/fonc.2015.00070/fullUbiquitinp62GIImmunohistochemical expressionCarcinomas |
| spellingShingle | Amr eMohamed Amr eMohamed Johnson eDeniece Charles eKovach Momin T. Siddiqui Cynthia eCohen P62/Ubiquitin IHC expression in gastrointestinal carcinomas Frontiers in Oncology Ubiquitin p62 GI Immunohistochemical expression Carcinomas |
| title | P62/Ubiquitin IHC expression in gastrointestinal carcinomas |
| title_full | P62/Ubiquitin IHC expression in gastrointestinal carcinomas |
| title_fullStr | P62/Ubiquitin IHC expression in gastrointestinal carcinomas |
| title_full_unstemmed | P62/Ubiquitin IHC expression in gastrointestinal carcinomas |
| title_short | P62/Ubiquitin IHC expression in gastrointestinal carcinomas |
| title_sort | p62 ubiquitin ihc expression in gastrointestinal carcinomas |
| topic | Ubiquitin p62 GI Immunohistochemical expression Carcinomas |
| url | http://journal.frontiersin.org/Journal/10.3389/fonc.2015.00070/full |
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