Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees
The detection throughout the year of latent and ILAR viruses in fruit trees by classical serological tests appears to be unreliable. Recently, these problems have smoothed themselves out by the use of molecular methods. We have developed RT-PCR protocols which are simple and reliable for routine det...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2002-06-01
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| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200210-0019_development-of-rt-pcr-tests-for-the-routine-detection-of-latent-and-ilar-viruses-in-fruit-trees.php |
| _version_ | 1797898651517845504 |
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| author | S. Marbot J. Kummert M. Salmon M. Vendrame A. Huwaert O. Dutrecq P. Lepoivre |
| author_facet | S. Marbot J. Kummert M. Salmon M. Vendrame A. Huwaert O. Dutrecq P. Lepoivre |
| author_sort | S. Marbot |
| collection | DOAJ |
| description | The detection throughout the year of latent and ILAR viruses in fruit trees by classical serological tests appears to be unreliable. Recently, these problems have smoothed themselves out by the use of molecular methods. We have developed RT-PCR protocols which are simple and reliable for routine detection of these viruses throughout the year. |
| first_indexed | 2024-04-10T08:16:29Z |
| format | Article |
| id | doaj.art-a25f55ba21de4ec59bb16bce9d64dfb4 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:16:29Z |
| publishDate | 2002-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-a25f55ba21de4ec59bb16bce9d64dfb42023-02-23T03:43:39ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412002-06-0138SI 1 - 6th Conf EFPPS21S2310.17221/10312-PPSpps-200210-0019Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit treesS. Marbot0J. Kummert1M. Salmon2M. Vendrame3A. Huwaert4O. Dutrecq5P. Lepoivre6Plant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.bePlant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.bePlant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.bePlant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.bePlant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.bePlant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.bePlant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.beThe detection throughout the year of latent and ILAR viruses in fruit trees by classical serological tests appears to be unreliable. Recently, these problems have smoothed themselves out by the use of molecular methods. We have developed RT-PCR protocols which are simple and reliable for routine detection of these viruses throughout the year.https://pps.agriculturejournals.cz/artkey/pps-200210-0019_development-of-rt-pcr-tests-for-the-routine-detection-of-latent-and-ilar-viruses-in-fruit-trees.phpaclsvaspvasgvpdvpnrsvapmvfruit treesrt-pcrrt-pcr-elosaminor groove binder-dna probe |
| spellingShingle | S. Marbot J. Kummert M. Salmon M. Vendrame A. Huwaert O. Dutrecq P. Lepoivre Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees Plant Protection Science aclsv aspv asgv pdv pnrsv apmv fruit trees rt-pcr rt-pcr-elosa minor groove binder-dna probe |
| title | Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees |
| title_full | Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees |
| title_fullStr | Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees |
| title_full_unstemmed | Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees |
| title_short | Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees |
| title_sort | development of rt pcr tests for the routine detection of latent and ilar viruses in fruit trees |
| topic | aclsv aspv asgv pdv pnrsv apmv fruit trees rt-pcr rt-pcr-elosa minor groove binder-dna probe |
| url | https://pps.agriculturejournals.cz/artkey/pps-200210-0019_development-of-rt-pcr-tests-for-the-routine-detection-of-latent-and-ilar-viruses-in-fruit-trees.php |
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