Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more
Background Microbial communities associated with animals are known to be key elements in the development of their hosts. In marine environments, these communities are largely under the influence of the surrounding water. In aquaculture, understanding the interactions existing between the microbiotas...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2021-11-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/12241.pdf |
| _version_ | 1797421832326873088 |
|---|---|
| author | Carolane Giraud Nolwenn Callac Maxime Beauvais Jean-René Mailliez Dominique Ansquer Nazha Selmaoui-Folcher Dominique Pham Nelly Wabete Viviane Boulo |
| author_facet | Carolane Giraud Nolwenn Callac Maxime Beauvais Jean-René Mailliez Dominique Ansquer Nazha Selmaoui-Folcher Dominique Pham Nelly Wabete Viviane Boulo |
| author_sort | Carolane Giraud |
| collection | DOAJ |
| description | Background Microbial communities associated with animals are known to be key elements in the development of their hosts. In marine environments, these communities are largely under the influence of the surrounding water. In aquaculture, understanding the interactions existing between the microbiotas of farmed species and their rearing environment could help establish precise bacterial management. Method In light of these facts, we studied the active microbial communities associated with the eggs and the nauplii of the Pacific blue shrimp (Litopenaeus stylirostris) and their rearing water. All samples were collected in September 2018, November 2018 and February 2019. After RNA extractions, two distinct Illumina HiSeq sequencings were performed. Due to different sequencing depths and in order to compare samples, data were normalized using the Count Per Million method. Results We found a core microbiota made of taxa related to Aestuariibacter, Alteromonas, Vibrio, SAR11, HIMB11, AEGEAN 169 marine group and Candidatus Endobugula associated with all the samples indicating that these bacterial communities could be transferred from the water to the animals. We also highlighted specific bacterial taxa in the eggs and the nauplii affiliated to Pseudomonas, Corynebacterium, Acinetobacter, Labrenzia, Rothia, Thalassolituus, Marinobacter, Aureispira, Oleiphilus, Profundimonas and Marinobacterium genera suggesting a possible prokaryotic vertical transmission from the breeders to their offspring. This study is the first to focus on the active microbiota associated with early developmental stages of a farmed shrimp species and could serve as a basis to comprehend the microbial interactions involved throughout the whole rearing process. |
| first_indexed | 2024-03-09T07:23:06Z |
| format | Article |
| id | doaj.art-a2612d3f3c2a4043a494f0b676274f73 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:23:06Z |
| publishDate | 2021-11-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-a2612d3f3c2a4043a494f0b676274f732023-12-03T07:14:01ZengPeerJ Inc.PeerJ2167-83592021-11-019e1224110.7717/peerj.12241Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and moreCarolane Giraud0Nolwenn Callac1Maxime Beauvais2Jean-René Mailliez3Dominique Ansquer4Nazha Selmaoui-Folcher5Dominique Pham6Nelly Wabete7Viviane Boulo8Ifremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, Noumea, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, Noumea, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, Noumea, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, Noumea, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, Noumea, New CaledoniaUniversity of New Caledonia, Institut des Sciences Exactes et Appliquées (ISEA), Noumea, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, Noumea, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, Noumea, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, Noumea, New CaledoniaBackground Microbial communities associated with animals are known to be key elements in the development of their hosts. In marine environments, these communities are largely under the influence of the surrounding water. In aquaculture, understanding the interactions existing between the microbiotas of farmed species and their rearing environment could help establish precise bacterial management. Method In light of these facts, we studied the active microbial communities associated with the eggs and the nauplii of the Pacific blue shrimp (Litopenaeus stylirostris) and their rearing water. All samples were collected in September 2018, November 2018 and February 2019. After RNA extractions, two distinct Illumina HiSeq sequencings were performed. Due to different sequencing depths and in order to compare samples, data were normalized using the Count Per Million method. Results We found a core microbiota made of taxa related to Aestuariibacter, Alteromonas, Vibrio, SAR11, HIMB11, AEGEAN 169 marine group and Candidatus Endobugula associated with all the samples indicating that these bacterial communities could be transferred from the water to the animals. We also highlighted specific bacterial taxa in the eggs and the nauplii affiliated to Pseudomonas, Corynebacterium, Acinetobacter, Labrenzia, Rothia, Thalassolituus, Marinobacter, Aureispira, Oleiphilus, Profundimonas and Marinobacterium genera suggesting a possible prokaryotic vertical transmission from the breeders to their offspring. This study is the first to focus on the active microbiota associated with early developmental stages of a farmed shrimp species and could serve as a basis to comprehend the microbial interactions involved throughout the whole rearing process.https://peerj.com/articles/12241.pdfActive microbiotaLineage transmissionEggsNaupliiShrimpsRearing water |
| spellingShingle | Carolane Giraud Nolwenn Callac Maxime Beauvais Jean-René Mailliez Dominique Ansquer Nazha Selmaoui-Folcher Dominique Pham Nelly Wabete Viviane Boulo Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more PeerJ Active microbiota Lineage transmission Eggs Nauplii Shrimps Rearing water |
| title | Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more |
| title_full | Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more |
| title_fullStr | Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more |
| title_full_unstemmed | Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more |
| title_short | Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more |
| title_sort | potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp litopenaeus stylirostris possible influence of the rearing water and more |
| topic | Active microbiota Lineage transmission Eggs Nauplii Shrimps Rearing water |
| url | https://peerj.com/articles/12241.pdf |
| work_keys_str_mv | AT carolanegiraud potentiallineagetransmissionwithintheactivemicrobiotaoftheeggsandthenaupliioftheshrimplitopenaeusstylirostrispossibleinfluenceoftherearingwaterandmore AT nolwenncallac potentiallineagetransmissionwithintheactivemicrobiotaoftheeggsandthenaupliioftheshrimplitopenaeusstylirostrispossibleinfluenceoftherearingwaterandmore AT maximebeauvais potentiallineagetransmissionwithintheactivemicrobiotaoftheeggsandthenaupliioftheshrimplitopenaeusstylirostrispossibleinfluenceoftherearingwaterandmore AT jeanrenemailliez potentiallineagetransmissionwithintheactivemicrobiotaoftheeggsandthenaupliioftheshrimplitopenaeusstylirostrispossibleinfluenceoftherearingwaterandmore AT dominiqueansquer potentiallineagetransmissionwithintheactivemicrobiotaoftheeggsandthenaupliioftheshrimplitopenaeusstylirostrispossibleinfluenceoftherearingwaterandmore AT nazhaselmaouifolcher potentiallineagetransmissionwithintheactivemicrobiotaoftheeggsandthenaupliioftheshrimplitopenaeusstylirostrispossibleinfluenceoftherearingwaterandmore AT dominiquepham potentiallineagetransmissionwithintheactivemicrobiotaoftheeggsandthenaupliioftheshrimplitopenaeusstylirostrispossibleinfluenceoftherearingwaterandmore AT nellywabete potentiallineagetransmissionwithintheactivemicrobiotaoftheeggsandthenaupliioftheshrimplitopenaeusstylirostrispossibleinfluenceoftherearingwaterandmore AT vivianeboulo potentiallineagetransmissionwithintheactivemicrobiotaoftheeggsandthenaupliioftheshrimplitopenaeusstylirostrispossibleinfluenceoftherearingwaterandmore |