The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice
The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pi...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622003723 |
| _version_ | 1811187850884939776 |
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| author | Irena Jovanovic Macura Ivana Djuricic Tamara Major Desanka Milanovic Marjana Brkic Sladjana Sobajic Selma Kanazir Sanja Ivkovic |
| author_facet | Irena Jovanovic Macura Ivana Djuricic Tamara Major Desanka Milanovic Marjana Brkic Sladjana Sobajic Selma Kanazir Sanja Ivkovic |
| author_sort | Irena Jovanovic Macura |
| collection | DOAJ |
| description | The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels. |
| first_indexed | 2024-04-11T14:10:31Z |
| format | Article |
| id | doaj.art-a262792a02464bfd92429eefe76bfb32 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-11T14:10:31Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-a262792a02464bfd92429eefe76bfb322022-12-22T04:19:45ZengElsevierJournal of Functional Foods1756-46462022-12-0199105302The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy miceIrena Jovanovic Macura0Ivana Djuricic1Tamara Major2Desanka Milanovic3Marjana Brkic4Sladjana Sobajic5Selma Kanazir6Sanja Ivkovic7Institute for Biological Research “Sinisa Stankovic”, National Institute of Republic of Serbia, University of Belgrade, SerbiaFaculty of Pharmacy, University of Belgrade, Belgrade, Serbia; Corresponding authors at: Vinca - Institute for Nuclear Sciences, Department of Molecular Biology and Endocrinology, National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia (S. Ivkovic). Department of Bromatology, Faculty of Pharmacy, Vojvode Stepe 450, 11221 Belgrade, Serbia (I. Djuricic).Faculty of Pharmacy, University of Belgrade, Belgrade, SerbiaInstitute for Biological Research “Sinisa Stankovic”, National Institute of Republic of Serbia, University of Belgrade, SerbiaInstitute for Biological Research “Sinisa Stankovic”, National Institute of Republic of Serbia, University of Belgrade, SerbiaFaculty of Pharmacy, University of Belgrade, Belgrade, SerbiaInstitute for Biological Research “Sinisa Stankovic”, National Institute of Republic of Serbia, University of Belgrade, SerbiaVinca - Institute for Nuclear Sciences, National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia; Corresponding authors at: Vinca - Institute for Nuclear Sciences, Department of Molecular Biology and Endocrinology, National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia (S. Ivkovic). Department of Bromatology, Faculty of Pharmacy, Vojvode Stepe 450, 11221 Belgrade, Serbia (I. Djuricic).The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.http://www.sciencedirect.com/science/article/pii/S1756464622003723Fish oilRetinaMfsd2aRPEEPADHA |
| spellingShingle | Irena Jovanovic Macura Ivana Djuricic Tamara Major Desanka Milanovic Marjana Brkic Sladjana Sobajic Selma Kanazir Sanja Ivkovic The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice Journal of Functional Foods Fish oil Retina Mfsd2a RPE EPA DHA |
| title | The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice |
| title_full | The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice |
| title_fullStr | The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice |
| title_full_unstemmed | The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice |
| title_short | The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice |
| title_sort | high dose fish oil supplementation increased mfsd2a expression without altering dha levels in the retina of healthy mice |
| topic | Fish oil Retina Mfsd2a RPE EPA DHA |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622003723 |
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