The LEPR (853A>G and 511A>G) Transitions may Enhance Idiopathic Recurrent Miscarriage: Evidences Based on Case-control and in silico Studies
Previous studies in human leptin receptor protein (LEPR) signaling are important in the establishment of fetal growth. Idiopathic recurrent miscarriage (IRM) may be the result of abnormal placental and fetal development. Thus single nucleotide polymorphisms (SNPs) of LEPR might be associated with IR...
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Ferdowsi University of Mashhad
2019-09-01
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| Series: | Journal of Cell and Molecular Research |
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| Online Access: | https://jcmr.um.ac.ir/article_29723_c1d6618e13c5c1a467613113d27a7ab4.pdf |
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| author | Abasalt Hosseinzadeh Colagar Masomeh Salehi-Doon |
| author_facet | Abasalt Hosseinzadeh Colagar Masomeh Salehi-Doon |
| author_sort | Abasalt Hosseinzadeh Colagar |
| collection | DOAJ |
| description | Previous studies in human leptin receptor protein (LEPR) signaling are important in the establishment of fetal growth. Idiopathic recurrent miscarriage (IRM) may be the result of abnormal placental and fetal development. Thus single nucleotide polymorphisms (SNPs) of LEPR might be associated with IRM. In our case-control study, which conducted from 2017 to 2018 at the Milad Sari Genetic Detection Center and Razi Hospital (Ghaemshahr, Iran), 140 samples, including 70 cases with history of three or more IRM as before the 22nd week of gestation, and 70 controls with at least two live births and no history of pathologic pregnancies during reproductive period were studied. Polymorphisms of maternal LEPR 853A>G and 511A>G were assessed by PCR-RFLP and SSCP, respectively. Results showed that 853A>G SNP, contained frequent genotype AG (p= 0.002; OR= 0.391; 95% CI= 0.154-0.664) and G allele (p= 0.003; OR= 0.125; 95% CI= 0.032–0.489), revealed a significant protective association with IRM. Primary screening of 511A>G showed that 63 case-samples were AG genotype. PCR directed sequence showed this SNP contained frequent genotype for AG (p= 0.001; OR= 0.57; 95% CI= 0.22-0.147) and G allele (p= 0.006; OR= 0.34; 95% CI= 0.008–0.149), revealed a significant protective association with IRM. Based on our findings, LEPR (853A>G and 511A>G) gene transitions not only might enhance IRM but also could be useful genetic markers in susceptibility and severity of recurrent miscarriage. |
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| issn | 2008-9147 2717-3364 |
| language | English |
| last_indexed | 2024-12-20T16:03:27Z |
| publishDate | 2019-09-01 |
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| series | Journal of Cell and Molecular Research |
| spelling | doaj.art-a2646aff49dc47449286c41eb67a01e52022-12-21T19:34:12ZengFerdowsi University of MashhadJournal of Cell and Molecular Research2008-91472717-33642019-09-01111233610.22067/jcmr.v11i1.8142629723The LEPR (853A>G and 511A>G) Transitions may Enhance Idiopathic Recurrent Miscarriage: Evidences Based on Case-control and in silico StudiesAbasalt Hosseinzadeh Colagar0Masomeh Salehi-Doon1University of Mazandaran, Babolsar, IranUniversity of Mazandaran, Babolsar, IranPrevious studies in human leptin receptor protein (LEPR) signaling are important in the establishment of fetal growth. Idiopathic recurrent miscarriage (IRM) may be the result of abnormal placental and fetal development. Thus single nucleotide polymorphisms (SNPs) of LEPR might be associated with IRM. In our case-control study, which conducted from 2017 to 2018 at the Milad Sari Genetic Detection Center and Razi Hospital (Ghaemshahr, Iran), 140 samples, including 70 cases with history of three or more IRM as before the 22nd week of gestation, and 70 controls with at least two live births and no history of pathologic pregnancies during reproductive period were studied. Polymorphisms of maternal LEPR 853A>G and 511A>G were assessed by PCR-RFLP and SSCP, respectively. Results showed that 853A>G SNP, contained frequent genotype AG (p= 0.002; OR= 0.391; 95% CI= 0.154-0.664) and G allele (p= 0.003; OR= 0.125; 95% CI= 0.032–0.489), revealed a significant protective association with IRM. Primary screening of 511A>G showed that 63 case-samples were AG genotype. PCR directed sequence showed this SNP contained frequent genotype for AG (p= 0.001; OR= 0.57; 95% CI= 0.22-0.147) and G allele (p= 0.006; OR= 0.34; 95% CI= 0.008–0.149), revealed a significant protective association with IRM. Based on our findings, LEPR (853A>G and 511A>G) gene transitions not only might enhance IRM but also could be useful genetic markers in susceptibility and severity of recurrent miscarriage.https://jcmr.um.ac.ir/article_29723_c1d6618e13c5c1a467613113d27a7ab4.pdflepr geneobesityrecurrent miscarriage |
| spellingShingle | Abasalt Hosseinzadeh Colagar Masomeh Salehi-Doon The LEPR (853A>G and 511A>G) Transitions may Enhance Idiopathic Recurrent Miscarriage: Evidences Based on Case-control and in silico Studies Journal of Cell and Molecular Research lepr gene obesity recurrent miscarriage |
| title | The LEPR (853A>G and 511A>G) Transitions may Enhance Idiopathic Recurrent Miscarriage: Evidences Based on Case-control and in silico Studies |
| title_full | The LEPR (853A>G and 511A>G) Transitions may Enhance Idiopathic Recurrent Miscarriage: Evidences Based on Case-control and in silico Studies |
| title_fullStr | The LEPR (853A>G and 511A>G) Transitions may Enhance Idiopathic Recurrent Miscarriage: Evidences Based on Case-control and in silico Studies |
| title_full_unstemmed | The LEPR (853A>G and 511A>G) Transitions may Enhance Idiopathic Recurrent Miscarriage: Evidences Based on Case-control and in silico Studies |
| title_short | The LEPR (853A>G and 511A>G) Transitions may Enhance Idiopathic Recurrent Miscarriage: Evidences Based on Case-control and in silico Studies |
| title_sort | lepr 853a g and 511a g transitions may enhance idiopathic recurrent miscarriage evidences based on case control and in silico studies |
| topic | lepr gene obesity recurrent miscarriage |
| url | https://jcmr.um.ac.ir/article_29723_c1d6618e13c5c1a467613113d27a7ab4.pdf |
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