Analysis of Let-7 Family miRNA in Plasma as Potential Predictive Biomarkers of Diagnosis for Papillary Thyroid Cancer
The most common histological type of thyroid cancer is papillary thyroid carcinoma (PTC). Radical resection of the thyroid gland is currently the recommended method of treatment. Almost 75% of thyroidectomies performed just for diagnostic purposes are benign. Thus, the confirmation of innovative and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/3/130 |
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| author | Ewelina Perdas Robert Stawski Krzysztof Kaczka Maria Zubrzycka |
| author_facet | Ewelina Perdas Robert Stawski Krzysztof Kaczka Maria Zubrzycka |
| author_sort | Ewelina Perdas |
| collection | DOAJ |
| description | The most common histological type of thyroid cancer is papillary thyroid carcinoma (PTC). Radical resection of the thyroid gland is currently the recommended method of treatment. Almost 75% of thyroidectomies performed just for diagnostic purposes are benign. Thus, the confirmation of innovative and more precise noninvasive biomarkers holds promise for the detection of PTC, which may decrease the number of unnecessary thyroid lobectomies. In this work, using the droplet digital PCR (ddPCR) method, we have analyzed the level of five miRNAs (let-7a, let-7c, let-7d, let-7f, and let-7i) in the plasma of patients with PTC and compared them with those of a healthy control group to investigate whether miRNAs also have value in the management of PTC. Levels of four miRNAs, namely let-7a, let-7c, let-7d, and let-7f, were significantly higher in PTC patients than healthy controls. Thus, the analysis of circulating let-7 can be a useful tool and support the currently used methods for PTC diagnosis. However, our observation requires further research on a larger patient group. |
| first_indexed | 2024-04-14T02:22:15Z |
| format | Article |
| id | doaj.art-a2682bded8804cbfa33455bb5dcaf2d5 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-04-14T02:22:15Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-a2682bded8804cbfa33455bb5dcaf2d52022-12-22T02:18:00ZengMDPI AGDiagnostics2075-44182020-02-0110313010.3390/diagnostics10030130diagnostics10030130Analysis of Let-7 Family miRNA in Plasma as Potential Predictive Biomarkers of Diagnosis for Papillary Thyroid CancerEwelina Perdas0Robert Stawski1Krzysztof Kaczka2Maria Zubrzycka3Department of Cardiovascular Physiology, Faculty of Medicine, Medical University of Lodz, 90-647 Lodz, PolandDepartment of Clinical Physiology, Faculty of Medicine, Medical University of Lodz, 90-647 Lodz, PolandDepartment of General and Oncological Surgery, Medical University of Lodz, 90-647 Lodz, PolandDepartment of Cardiovascular Physiology, Faculty of Medicine, Medical University of Lodz, 90-647 Lodz, PolandThe most common histological type of thyroid cancer is papillary thyroid carcinoma (PTC). Radical resection of the thyroid gland is currently the recommended method of treatment. Almost 75% of thyroidectomies performed just for diagnostic purposes are benign. Thus, the confirmation of innovative and more precise noninvasive biomarkers holds promise for the detection of PTC, which may decrease the number of unnecessary thyroid lobectomies. In this work, using the droplet digital PCR (ddPCR) method, we have analyzed the level of five miRNAs (let-7a, let-7c, let-7d, let-7f, and let-7i) in the plasma of patients with PTC and compared them with those of a healthy control group to investigate whether miRNAs also have value in the management of PTC. Levels of four miRNAs, namely let-7a, let-7c, let-7d, and let-7f, were significantly higher in PTC patients than healthy controls. Thus, the analysis of circulating let-7 can be a useful tool and support the currently used methods for PTC diagnosis. However, our observation requires further research on a larger patient group.https://www.mdpi.com/2075-4418/10/3/130ptcmirnalet-7ddpcrdiagnostics |
| spellingShingle | Ewelina Perdas Robert Stawski Krzysztof Kaczka Maria Zubrzycka Analysis of Let-7 Family miRNA in Plasma as Potential Predictive Biomarkers of Diagnosis for Papillary Thyroid Cancer Diagnostics ptc mirna let-7 ddpcr diagnostics |
| title | Analysis of Let-7 Family miRNA in Plasma as Potential Predictive Biomarkers of Diagnosis for Papillary Thyroid Cancer |
| title_full | Analysis of Let-7 Family miRNA in Plasma as Potential Predictive Biomarkers of Diagnosis for Papillary Thyroid Cancer |
| title_fullStr | Analysis of Let-7 Family miRNA in Plasma as Potential Predictive Biomarkers of Diagnosis for Papillary Thyroid Cancer |
| title_full_unstemmed | Analysis of Let-7 Family miRNA in Plasma as Potential Predictive Biomarkers of Diagnosis for Papillary Thyroid Cancer |
| title_short | Analysis of Let-7 Family miRNA in Plasma as Potential Predictive Biomarkers of Diagnosis for Papillary Thyroid Cancer |
| title_sort | analysis of let 7 family mirna in plasma as potential predictive biomarkers of diagnosis for papillary thyroid cancer |
| topic | ptc mirna let-7 ddpcr diagnostics |
| url | https://www.mdpi.com/2075-4418/10/3/130 |
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